##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AgeI ApoI AscI AseI AsnI
Asp700I Asp718I AspI BamHI BbrPI
BcgI BclI BglII BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MflI
MluI MroI MslI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 2008 AACGTT AA^CGTT
AcyI 2185 GGCGCC G[GA]^CG[CT]C
AflII 573 CTTAAG C^TTAAG
AflIII 2618 ACGTGT A^C[GA][CT]GT
AhdI 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1728 AGCGCT AGC^GCT
AspEI 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 497 CCTAGG C^CTAGG
BbeI 2185 GGCGCC GGCGC^C
BfrI 573 CTTAAG C^TTAAG
BglI 762 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 497 CCTAGG C^CTAGG
BsaHI 2185 GGCGCC G[GA]^CG[CT]C
BsgI 3036 CTGCAC GTGCAG(16/14)
BsmBI 3788 GAGACG CGTCTC(1/5)
Eam1105I 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1728 AGCGCT AGC^GCT
EcoT22I 2268 ATGCAT ATGCA^T
HgaI 1518 GCGTC GACGC(5/10)
Hin1I 2185 GGCGCC G[GA]^CG[CT]C
KasI 2185 GGCGCC G^GCGCC
MfeI 1878 CAATTG C^AATTG
MunI 1878 CAATTG C^AATTG
NarI 2185 GGCGCC GG^CGCC
NheI 2691 GCTAGC G^CTAGC
NsiI 2268 ATGCAT ATGCA^T
Psp1406I 2008 AACGTT AA^CGTT
SbfI 2453 CCTGCAGG CCTGCA^GG
ScaI 3032 AGTACT AGT^ACT
SfoI 2185 GGCGCC GGC^GCC
SmaI 41 CCCGGG CCC^GGG
Sse8387I 2453 CCTGCAGG CCTGCA^GG
Tsp509I 1879 AATT ^AATT
XcmI 3621 CCAGAGGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1812 CGCG CG^CG
2670 CGCG
Alw44I 2464 GTGCAC G^TGCAC
2622 GTGCAC
AlwI 3810 GATCC GGATC(4/5)
1664 GGATC
AocI 483 CCTAAGG CC^T[ACGT]AGG
876 CCTAAGG
ApaLI 2464 GTGCAC G^TGCAC
2622 GTGCAC
AvaI 41 CCCGGG C^[CT]CG[GA]G
3469 CTCGGG
BalI 1582 TGGCCA TGG^CCA
3368 TGGCCA
BciVI 89 GGATAC GTATCC(6/5)
1221 GGATAC
BsaI 193 GAGACC GGTCTC(1/5)
381 GAGACC
BsoBI 41 CCCGGG C^[CT]CG[GA]G
3469 CTCGGG
BsrFI 1990 GCCGGC [GA]^CCGG[CT]
2566 GCCGGC
BstUI 1812 CGCG CG^CG
2670 CGCG
Bsu36I 483 CCTAAGG CC^T[ACGT]AGG
876 CCTAAGG
Cfr10I 1990 GCCGGC [GA]^CCGG[CT]
2566 GCCGGC
DrdI 1304 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3270 GACAGTAGGGTC
Eco81I 483 CCTAAGG CC^T[ACGT]AGG
876 CCTAAGG
MaeII 2009 ACGT A^CGT
2618 ACGT
MluNI 1582 TGGCCA TGG^CCA
3368 TGGCCA
MscI 1582 TGGCCA TGG^CCA
3368 TGGCCA
MvnI 1812 CGCG CG^CG
2670 CGCG
NaeI 1990 GCCGGC GCC^GGC
2566 GCCGGC
NcoI 1585 CCATGG C^CATGG
2098 CCATGG
NgoMIV 1990 GCCGGC G^CCGGC
2566 GCCGGC
NspI 1514 GCATGC [GA]CATG^[CT]
2270 GCATGC
PflMI 276 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1027 CCATGAGTTGG
SmlI 573 CTTAAG C^T[CT][GA]AG
945 CTTGAG
SphI 1514 GCATGC GCATG^C
2270 GCATGC
Van91I 276 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1027 CCATGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaII AviII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I