################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AccIII AcsI AgeI ApoI AscI AseI AsnI Asp700I Asp718I AspI BamHI BbrPI BcgI BclI BglII BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MflI MluI MroI MslI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AclI 2008 AACGTT AA^CGTT AcyI 2185 GGCGCC G[GA]^CG[CT]C AflII 573 CTTAAG C^TTAAG AflIII 2618 ACGTGT A^C[GA][CT]GT AhdI 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1728 AGCGCT AGC^GCT AspEI 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 497 CCTAGG C^CTAGG BbeI 2185 GGCGCC GGCGC^C BfrI 573 CTTAAG C^TTAAG BglI 762 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 497 CCTAGG C^CTAGG BsaHI 2185 GGCGCC G[GA]^CG[CT]C BsgI 3036 CTGCAC GTGCAG(16/14) BsmBI 3788 GAGACG CGTCTC(1/5) Eam1105I 591 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1728 AGCGCT AGC^GCT EcoT22I 2268 ATGCAT ATGCA^T HgaI 1518 GCGTC GACGC(5/10) Hin1I 2185 GGCGCC G[GA]^CG[CT]C KasI 2185 GGCGCC G^GCGCC MfeI 1878 CAATTG C^AATTG MunI 1878 CAATTG C^AATTG NarI 2185 GGCGCC GG^CGCC NheI 2691 GCTAGC G^CTAGC NsiI 2268 ATGCAT ATGCA^T Psp1406I 2008 AACGTT AA^CGTT SbfI 2453 CCTGCAGG CCTGCA^GG ScaI 3032 AGTACT AGT^ACT SfoI 2185 GGCGCC GGC^GCC SmaI 41 CCCGGG CCC^GGG Sse8387I 2453 CCTGCAGG CCTGCA^GG Tsp509I 1879 AATT ^AATT XcmI 3621 CCAGAGGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 41 CCCGGG C^CCGGG XmaI 41 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 1812 CGCG CG^CG 2670 CGCG Alw44I 2464 GTGCAC G^TGCAC 2622 GTGCAC AlwI 3810 GATCC GGATC(4/5) 1664 GGATC AocI 483 CCTAAGG CC^T[ACGT]AGG 876 CCTAAGG ApaLI 2464 GTGCAC G^TGCAC 2622 GTGCAC AvaI 41 CCCGGG C^[CT]CG[GA]G 3469 CTCGGG BalI 1582 TGGCCA TGG^CCA 3368 TGGCCA BciVI 89 GGATAC GTATCC(6/5) 1221 GGATAC BsaI 193 GAGACC GGTCTC(1/5) 381 GAGACC BsoBI 41 CCCGGG C^[CT]CG[GA]G 3469 CTCGGG BsrFI 1990 GCCGGC [GA]^CCGG[CT] 2566 GCCGGC BstUI 1812 CGCG CG^CG 2670 CGCG Bsu36I 483 CCTAAGG CC^T[ACGT]AGG 876 CCTAAGG Cfr10I 1990 GCCGGC [GA]^CCGG[CT] 2566 GCCGGC DrdI 1304 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3270 GACAGTAGGGTC Eco81I 483 CCTAAGG CC^T[ACGT]AGG 876 CCTAAGG MaeII 2009 ACGT A^CGT 2618 ACGT MluNI 1582 TGGCCA TGG^CCA 3368 TGGCCA MscI 1582 TGGCCA TGG^CCA 3368 TGGCCA MvnI 1812 CGCG CG^CG 2670 CGCG NaeI 1990 GCCGGC GCC^GGC 2566 GCCGGC NcoI 1585 CCATGG C^CATGG 2098 CCATGG NgoMIV 1990 GCCGGC G^CCGGC 2566 GCCGGC NspI 1514 GCATGC [GA]CATG^[CT] 2270 GCATGC PflMI 276 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1027 CCATGAGTTGG SmlI 573 CTTAAG C^T[CT][GA]AG 945 CTTGAG SphI 1514 GCATGC GCATG^C 2270 GCATGC Van91I 276 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1027 CCATGAGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AspHI AvaII AviII BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I