##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaI ApaLI AscI BbeI BbvCI
BcgI BciVI BlpI Bpu1102I BsaBI
BsaWI BseAI BseRI BsiWI BsmI
BspEI BsrBI BsrFI BssHII BssSI
BstEII BstPI CelII Cfr10I CpoI
EagI EclXI Eco52I EcoNI EcoO65I
EcoT22I FseI HpaI KasI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PstI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 2906 GGTACC G^GTACC
AlwNI 1963 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2201 ATTAAT AT^TAAT
AsnI 2201 ATTAAT AT^TAAT
Asp718I 2906 GGTACC G^GTACC
AspI 3732 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1750 TGCGCA TGC^GCA
BalI 239 TGGCCA TGG^CCA
BamHI 1243 GGATCC G^GATCC
BbsI 3058 GTCTTC GAAGAC(2/6)
BclI 338 TGATCA T^GATCA
BcnI 2413 CCCGG CC^[GC]GG
BglI 1304 GCCACCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3086 AGATCT A^GATCT
BpuAI 3058 GTCTTC GAAGAC(2/6)
BsaI 248 GAGACC GGTCTC(1/5)
BsiEI 1509 CGATCG CG[GA][CT]^CG
BsmBI 3714 GAGACG CGTCTC(1/5)
BspDI 1511 ATCGAT AT^CGAT
BspHI 2764 TCATGA T^CATGA
BspLU11I 498 ACATGT A^CATGT
Bst1107I 4050 GTATAC GTA^TAC
BstAPI 213 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4105 TTCGAA TT^CGAA
BstXI 726 CCAGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4050 GTATAC GTA^TAC
ClaI 1511 ATCGAT AT^CGAT
DraII 1879 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 4154 GACAGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 537 CCATGG C^C[GA][CT]GG
EaeI 239 TGGCCA [CT]^GGCC[GA]
EcoO109I 1879 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 3888 GAATTC G^AATTC
EcoRV 3631 GATATC GAT^ATC
FbaI 338 TGATCA T^GATCA
FspI 1750 TGCGCA TGC^GCA
HapII 2414 CCGG C^CGG
HincII 4360 GTCAAC GT[CT]^[GA]AC
HindII 4360 GTCAAC GT[CT]^[GA]AC
HpaII 2414 CCGG C^CGG
KpnI 2906 GGTACC GGTAC^C
MfeI 452 CAATTG C^AATTG
MluNI 239 TGGCCA TGG^CCA
MscI 239 TGGCCA TGG^CCA
MspI 2414 CCGG C^CGG
MunI 452 CAATTG C^AATTG
NciI 2413 CCCGG CC^[GC]GG
NcoI 537 CCATGG C^CATGG
NdeI 374 CATATG CA^TATG
NspV 4105 TTCGAA TT^CGAA
PflFI 3732 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1879 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2201 ATTAAT AT^TAAT
PvuI 1509 CGATCG CGAT^CG
RcaI 2764 TCATGA T^CATGA
ScaI 1115 AGTACT AGT^ACT
SexAI 158 ACCTGGT A^CC[AT]GGT
SfuI 4105 TTCGAA TT^CGAA
SphI 1842 GCATGC GCATG^C
SwaI 3206 ATTTAAAT ATTT^AAAT
Tth111I 3732 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3114 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 3080 GGCGTC G[GA]^CG[CT]C
4133 GGCGTC
AhdI 457 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1440 GACAGAGTGTC
AocI 487 CCTCAGG CC^T[ACGT]AGG
3527 CCTTAGG
Aor51HI 1850 AGCGCT AGC^GCT
1956 AGCGCT
Asp700I 1096 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4144 GAACTGCTTC
AspEI 457 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1440 GACAGAGTGTC
AvrII 1885 CCTAGG C^CTAGG
2113 CCTAGG
BanI 1832 GGCACC G^G[CT][GA]CC
2906 GGTACC
BbrPI 1667 CACGTG CAC^GTG
2151 CACGTG
BlnI 1885 CCTAGG C^CTAGG
2113 CCTAGG
BsaHI 3080 GGCGTC G[GA]^CG[CT]C
4133 GGCGTC
BsgI 2127 GTGCAG GTGCAG(16/14)
2781 GTGCAG
BsrDI 2082 CATTGC GCAATG(2/0)
208 GCAATG
Bsu36I 487 CCTCAGG CC^T[ACGT]AGG
3527 CCTTAGG
DraI 3207 TTTAAA TTT^AAA
3305 TTTAAA
Eam1105I 457 GACTGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1440 GACAGAGTGTC
Eco47III 1850 AGCGCT AGC^GCT
1956 AGCGCT
Eco81I 487 CCTCAGG CC^T[ACGT]AGG
3527 CCTTAGG
FauI 222 GCGGG CCCGC(4/6)
519 GCGGG
Hin1I 3080 GGCGTC G[GA]^CG[CT]C
4133 GGCGTC
MspA1I 820 CAGCTG C[AC]G^C[GT]G
836 CAGCTG
PleI 1672 GACTC GAGTC(4/5)
1895 GAGTC
PmaCI 1667 CACGTG CAC^GTG
2151 CACGTG
PmlI 1667 CACGTG CAC^GTG
2151 CACGTG
PvuII 820 CAGCTG CAG^CTG
836 CAGCTG
SacI 3688 GAGCTC GAGCT^C
3793 GAGCTC
SspI 2687 AATATT AAT^ATT
3285 AATATT
StuI 1566 AGGCCT AGG^CCT
2554 AGGCCT
XmnI 1096 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4144 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AflIII AluI AlwI ApoI
AspHI AvaI AvaII BanII BbvI
BfaI BfrI BmyI BpmI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraIII EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HgaI HhaI HinP1I HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PflMI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII