################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AhdI Alw44I ApaLI ApoI AscI AseI AsnI AspEI AviII BbsI BcgI BciVI BclI BfrI BpuAI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BstBI BstUI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SfiI SfuI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 949 GGTACC G^GTACC AflIII 2047 ACACGT A^C[GA][CT]GT Aor51HI 2439 AGCGCT AGC^GCT Asp718I 949 GGTACC G^GTACC AspI 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2048 CACGTG CAC^GTG BbvCI 1818 CCTCAGC CCTCAGC(-5/-2) BsmBI 1865 GAGACG CGTCTC(1/5) Bsp1407I 3720 TGTACA T^GTACA BsrGI 3720 TGTACA T^GTACA Bst1107I 1346 GTATAC GTA^TAC BstZ17I 1346 GTATAC GTA^TAC DraI 4167 TTTAAA TTT^AAA Eco47III 2439 AGCGCT AGC^GCT EcoRV 905 GATATC GAT^ATC HincII 1029 GTCGAC GT[CT]^[GA]AC HindII 1029 GTCGAC GT[CT]^[GA]AC KpnI 949 GGTACC GGTAC^C MseI 4168 TTAA T^TAA NaeI 87 GCCGGC GCC^GGC NgoMIV 87 GCCGGC G^CCGGC PflFI 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2048 CACGTG CAC^GTG PmlI 2048 CACGTG CAC^GTG PshAI 1022 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1949 GAGCTC GAGCT^C SalI 1029 GTCGAC G^TCGAC ScaI 329 AGTACT AGT^ACT SgrAI 3734 CACCGGTG C[GA]^CCGG[CT]G SmlI 4222 CTTGAG C^T[CT][GA]AG SspBI 3720 TGTACA T^GTACA Tru9I 4168 TTAA T^TAA Tth111I 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1029 GTCGAC GT^[AC][GT]AC 1346 GTATAC AgeI 514 ACCGGT A^CCGGT 3735 ACCGGT AocI 1467 CCTTAGG CC^T[ACGT]AGG 2345 CCTCAGG Asp700I 1492 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2761 GAACAGCTTC BamHI 654 GGATCC G^GATCC 2673 GGATCC BglII 750 AGATCT A^GATCT 1138 AGATCT BsaAI 2048 CACGTG [CT]AC^GT[GA] 2228 TACGTG BsrBI 629 GAGCGG CCGCTC(-3/-3) 3082 GAGCGG BsrDI 1603 CATTGC GCAATG(2/0) 2387 CATTGC BssSI 1096 CTCGTG CACGAG(-5/-1) 3502 CTCGTG BstEII 232 GGTCACC G^GT[ACGT]ACC 1597 GGTCACC BstPI 232 GGTCACC G^GT[ACGT]ACC 1597 GGTCACC Bsu36I 1467 CCTTAGG CC^T[ACGT]AGG 2345 CCTCAGG DrdI 1032 GACAAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2461 GACGGTTCTGTC Eco81I 1467 CCTTAGG CC^T[ACGT]AGG 2345 CCTCAGG EcoO65I 232 GGTCACC G^GT[ACGT]ACC 1597 GGTCACC PinAI 514 ACCGGT A^CCGGT 3735 ACCGGT PpuMI 1530 AGGACCC [GA]G^G[AT]CC[CT] 2897 GGGTCCT SexAI 1124 ACCTGGT A^CC[AT]GGT 3960 ACCAGGT SmaI 1939 CCCGGG CCC^GGG 2702 CCCGGG SphI 1678 GCATGC GCATG^C 2646 GCATGC StuI 258 AGGCCT AGG^CCT 3415 AGGCCT Tsp509I 875 AATT ^AATT 2177 AATT XcmI 152 CCATCCTGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2715 CCAACATCACCATGG XmaCI 1939 CCCGGG C^CCGGG 2702 CCCGGG XmaI 1939 CCCGGG C^CCGGG 2702 CCCGGG XmnI 1492 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2761 GAACAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII