##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AviII
BbsI BcgI BciVI BclI BfrI
BpuAI BsaBI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BssHII BstBI BstUI ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI MvnI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
SfiI SfuI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 949 GGTACC G^GTACC
AflIII 2047 ACACGT A^C[GA][CT]GT
Aor51HI 2439 AGCGCT AGC^GCT
Asp718I 949 GGTACC G^GTACC
AspI 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2048 CACGTG CAC^GTG
BbvCI 1818 CCTCAGC CCTCAGC(-5/-2)
BsmBI 1865 GAGACG CGTCTC(1/5)
Bsp1407I 3720 TGTACA T^GTACA
BsrGI 3720 TGTACA T^GTACA
Bst1107I 1346 GTATAC GTA^TAC
BstZ17I 1346 GTATAC GTA^TAC
DraI 4167 TTTAAA TTT^AAA
Eco47III 2439 AGCGCT AGC^GCT
EcoRV 905 GATATC GAT^ATC
HincII 1029 GTCGAC GT[CT]^[GA]AC
HindII 1029 GTCGAC GT[CT]^[GA]AC
KpnI 949 GGTACC GGTAC^C
MseI 4168 TTAA T^TAA
NaeI 87 GCCGGC GCC^GGC
NgoMIV 87 GCCGGC G^CCGGC
PflFI 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2048 CACGTG CAC^GTG
PmlI 2048 CACGTG CAC^GTG
PshAI 1022 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1949 GAGCTC GAGCT^C
SalI 1029 GTCGAC G^TCGAC
ScaI 329 AGTACT AGT^ACT
SgrAI 3734 CACCGGTG C[GA]^CCGG[CT]G
SmlI 4222 CTTGAG C^T[CT][GA]AG
SspBI 3720 TGTACA T^GTACA
Tru9I 4168 TTAA T^TAA
Tth111I 2 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1029 GTCGAC GT^[AC][GT]AC
1346 GTATAC
AgeI 514 ACCGGT A^CCGGT
3735 ACCGGT
AocI 1467 CCTTAGG CC^T[ACGT]AGG
2345 CCTCAGG
Asp700I 1492 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2761 GAACAGCTTC
BamHI 654 GGATCC G^GATCC
2673 GGATCC
BglII 750 AGATCT A^GATCT
1138 AGATCT
BsaAI 2048 CACGTG [CT]AC^GT[GA]
2228 TACGTG
BsrBI 629 GAGCGG CCGCTC(-3/-3)
3082 GAGCGG
BsrDI 1603 CATTGC GCAATG(2/0)
2387 CATTGC
BssSI 1096 CTCGTG CACGAG(-5/-1)
3502 CTCGTG
BstEII 232 GGTCACC G^GT[ACGT]ACC
1597 GGTCACC
BstPI 232 GGTCACC G^GT[ACGT]ACC
1597 GGTCACC
Bsu36I 1467 CCTTAGG CC^T[ACGT]AGG
2345 CCTCAGG
DrdI 1032 GACAAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACGGTTCTGTC
Eco81I 1467 CCTTAGG CC^T[ACGT]AGG
2345 CCTCAGG
EcoO65I 232 GGTCACC G^GT[ACGT]ACC
1597 GGTCACC
PinAI 514 ACCGGT A^CCGGT
3735 ACCGGT
PpuMI 1530 AGGACCC [GA]G^G[AT]CC[CT]
2897 GGGTCCT
SexAI 1124 ACCTGGT A^CC[AT]GGT
3960 ACCAGGT
SmaI 1939 CCCGGG CCC^GGG
2702 CCCGGG
SphI 1678 GCATGC GCATG^C
2646 GCATGC
StuI 258 AGGCCT AGG^CCT
3415 AGGCCT
Tsp509I 875 AATT ^AATT
2177 AATT
XcmI 152 CCATCCTGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2715 CCAACATCACCATGG
XmaCI 1939 CCCGGG C^CCGGG
2702 CCCGGG
XmaI 1939 CCCGGG C^CCGGG
2702 CCCGGG
XmnI 1492 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2761 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
AvrII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII