##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI AspEI AviII
AvrII BbrPI BcgI BciVI BclI
BlnI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrGI BssHII BstBI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KspI MluI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SphI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1326 GGTACC G^GTACC
AccII 100 CGCG CG^CG
AccIII 539 TCCGGA T^CCGGA
AclI 4346 AACGTT AA^CGTT
AcyI 572 GGCGCC G[GA]^CG[CT]C
AflII 957 CTTAAG C^TTAAG
Alw44I 2040 GTGCAC G^TGCAC
ApaLI 2040 GTGCAC G^TGCAC
Asp718I 1326 GGTACC G^GTACC
AspI 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 92 CCCGGG C^[CT]CG[GA]G
BbeI 572 GGCGCC GGCGC^C
BbvCI 1703 GCTGAGG CCTCAGC(-5/-2)
BfrI 957 CTTAAG C^TTAAG
BglII 3936 AGATCT A^GATCT
BsaAI 4087 TACGTA [CT]AC^GT[GA]
BsaHI 572 GGCGCC G[GA]^CG[CT]C
BseAI 539 TCCGGA T^CCGGA
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspEI 539 TCCGGA T^CCGGA
BsrFI 2375 GCCGGC [GA]^CCGG[CT]
BssSI 433 CTCGTG CACGAG(-5/-1)
Bst1107I 4276 GTATAC GTA^TAC
BstEII 1836 GGTGACC G^GT[ACGT]ACC
BstPI 1836 GGTGACC G^GT[ACGT]ACC
BstUI 100 CGCG CG^CG
BstZ17I 4276 GTATAC GTA^TAC
Cfr10I 2375 GCCGGC [GA]^CCGG[CT]
DraIII 1000 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1836 GGTGACC G^GT[ACGT]ACC
EcoT22I 3449 ATGCAT ATGCA^T
HaeII 572 GGCGCC [GA]GCGC^[CT]
Hin1I 572 GGCGCC G[GA]^CG[CT]C
KasI 572 GGCGCC G^GCGCC
KpnI 1326 GGTACC GGTAC^C
MroI 539 TCCGGA T^CCGGA
MvnI 100 CGCG CG^CG
NaeI 2375 GCCGGC GCC^GGC
NarI 572 GGCGCC GG^CGCC
NgoMIV 2375 GCCGGC G^CCGGC
NsiI 3449 ATGCAT ATGCA^T
PflFI 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4346 AACGTT AA^CGTT
SacI 1934 GAGCTC GAGCT^C
SapI 3014 GAAGAGC GCTCTTC(1/4)
SexAI 369 ACCTGGT A^CC[AT]GGT
SfoI 572 GGCGCC GGC^GCC
SmaI 92 CCCGGG CCC^GGG
SnaBI 4087 TACGTA TAC^GTA
SpeI 2631 ACTAGT A^CTAGT
Tth111I 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3297 GTAGAC GT^[AC][GT]AC
4276 GTATAC
AflIII 2093 ACATGT A^C[GA][CT]GT
4335 ACATGT
AlwNI 1917 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
3962 CAGCCACTG
AocI 618 CCTGAGG CC^T[ACGT]AGG
1956 CCTTAGG
Asp700I 800 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3934 GAAGATCTTC
BalI 1153 TGGCCA TGG^CCA
3775 TGGCCA
BamHI 31 GGATCC G^GATCC
4022 GGATCC
BglI 1043 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3232 GCCAACTGGGC
BsaBI 3931 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4467 GATGTCTATC
BsaI 842 GGTCTC GGTCTC(1/5)
4233 GGTCTC
BsaWI 539 TCCGGA [AT]^CCGG[AT]
2934 ACCGGA
BsmBI 387 CGTCTC CGTCTC(1/5)
4121 GAGACG
BspLU11I 2093 ACATGT A^CATGT
4335 ACATGT
Bsu36I 618 CCTGAGG CC^T[ACGT]AGG
1956 CCTTAGG
CfoI 101 GCGC GCG^C
573 GCGC
EaeI 1153 TGGCCA [CT]^GGCC[GA]
3775 TGGCCA
Eco81I 618 CCTGAGG CC^T[ACGT]AGG
1956 CCTTAGG
EcoRV 318 GATATC GAT^ATC
1482 GATATC
HhaI 101 GCGC GCG^C
573 GCGC
HinP1I 101 GCGC G^CGC
573 GCGC
MamI 3931 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4467 GATGTCTATC
MfeI 2028 CAATTG C^AATTG
2751 CAATTG
MluNI 1153 TGGCCA TGG^CCA
3775 TGGCCA
MscI 1153 TGGCCA TGG^CCA
3775 TGGCCA
MunI 2028 CAATTG C^AATTG
2751 CAATTG
PpuMI 764 AGGTCCT [GA]G^G[AT]CC[CT]
3004 AGGACCT
PshAI 138 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2700 GACCATGGTC
SspI 1730 AATATT AAT^ATT
2339 AATATT
StuI 522 AGGCCT AGG^CCT
3202 AGGCCT
XcmI 1141 CCAAAGAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2978 CCATGGGGTTGATGG
XmnI 800 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3934 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII