##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbrPI       BcgI        BciVI       BclI        
BlnI        BsgI        BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspHI       BsrBI       BsrDI       
BsrGI       BssHII      BstBI       ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KspI        MluI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfuI        SgrAI       SphI        Sse8387I    SspBI       
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1326  GGTACC               G^GTACC
AccII         100  CGCG                 CG^CG
AccIII        539  TCCGGA               T^CCGGA
AclI         4346  AACGTT               AA^CGTT
AcyI          572  GGCGCC               G[GA]^CG[CT]C
AflII         957  CTTAAG               C^TTAAG
Alw44I       2040  GTGCAC               G^TGCAC
ApaLI        2040  GTGCAC               G^TGCAC
Asp718I      1326  GGTACC               G^GTACC
AspI          559  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           92  CCCGGG               C^[CT]CG[GA]G
BbeI          572  GGCGCC               GGCGC^C
BbvCI        1703  GCTGAGG              CCTCAGC(-5/-2)
BfrI          957  CTTAAG               C^TTAAG
BglII        3936  AGATCT               A^GATCT
BsaAI        4087  TACGTA               [CT]AC^GT[GA]
BsaHI         572  GGCGCC               G[GA]^CG[CT]C
BseAI         539  TCCGGA               T^CCGGA
BsoBI          92  CCCGGG               C^[CT]CG[GA]G
BspEI         539  TCCGGA               T^CCGGA
BsrFI        2375  GCCGGC               [GA]^CCGG[CT]
BssSI         433  CTCGTG               CACGAG(-5/-1)
Bst1107I     4276  GTATAC               GTA^TAC
BstEII       1836  GGTGACC              G^GT[ACGT]ACC
BstPI        1836  GGTGACC              G^GT[ACGT]ACC
BstUI         100  CGCG                 CG^CG
BstZ17I      4276  GTATAC               GTA^TAC
Cfr10I       2375  GCCGGC               [GA]^CCGG[CT]
DraIII       1000  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1836  GGTGACC              G^GT[ACGT]ACC
EcoT22I      3449  ATGCAT               ATGCA^T
HaeII         572  GGCGCC               [GA]GCGC^[CT]
Hin1I         572  GGCGCC               G[GA]^CG[CT]C
KasI          572  GGCGCC               G^GCGCC
KpnI         1326  GGTACC               GGTAC^C
MroI          539  TCCGGA               T^CCGGA
MvnI          100  CGCG                 CG^CG
NaeI         2375  GCCGGC               GCC^GGC
NarI          572  GGCGCC               GG^CGCC
NgoMIV       2375  GCCGGC               G^CCGGC
NsiI         3449  ATGCAT               ATGCA^T
PflFI         559  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     4346  AACGTT               AA^CGTT
SacI         1934  GAGCTC               GAGCT^C
SapI         3014  GAAGAGC              GCTCTTC(1/4)
SexAI         369  ACCTGGT              A^CC[AT]GGT
SfoI          572  GGCGCC               GGC^GCC
SmaI           92  CCCGGG               CCC^GGG
SnaBI        4087  TACGTA               TAC^GTA
SpeI         2631  ACTAGT               A^CTAGT
Tth111I       559  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          92  CCCGGG               C^CCGGG
XmaI           92  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         3297  GTAGAC               GT^[AC][GT]AC
             4276  GTATAC              
AflIII       2093  ACATGT               A^C[GA][CT]GT
             4335  ACATGT              
AlwNI        1917  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3962  CAGCCACTG           
AocI          618  CCTGAGG              CC^T[ACGT]AGG
             1956  CCTTAGG             
Asp700I       800  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3934  GAAGATCTTC          
BalI         1153  TGGCCA               TGG^CCA
             3775  TGGCCA              
BamHI          31  GGATCC               G^GATCC
             4022  GGATCC              
BglI         1043  GCCCCAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3232  GCCAACTGGGC         
BsaBI        3931  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4467  GATGTCTATC          
BsaI          842  GGTCTC               GGTCTC(1/5)
             4233  GGTCTC              
BsaWI         539  TCCGGA               [AT]^CCGG[AT]
             2934  ACCGGA              
BsmBI         387  CGTCTC               CGTCTC(1/5)
             4121  GAGACG              
BspLU11I     2093  ACATGT               A^CATGT
             4335  ACATGT              
Bsu36I        618  CCTGAGG              CC^T[ACGT]AGG
             1956  CCTTAGG             
CfoI          101  GCGC                 GCG^C
              573  GCGC                
EaeI         1153  TGGCCA               [CT]^GGCC[GA]
             3775  TGGCCA              
Eco81I        618  CCTGAGG              CC^T[ACGT]AGG
             1956  CCTTAGG             
EcoRV         318  GATATC               GAT^ATC
             1482  GATATC              
HhaI          101  GCGC                 GCG^C
              573  GCGC                
HinP1I        101  GCGC                 G^CGC
              573  GCGC                
MamI         3931  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4467  GATGTCTATC          
MfeI         2028  CAATTG               C^AATTG
             2751  CAATTG              
MluNI        1153  TGGCCA               TGG^CCA
             3775  TGGCCA              
MscI         1153  TGGCCA               TGG^CCA
             3775  TGGCCA              
MunI         2028  CAATTG               C^AATTG
             2751  CAATTG              
PpuMI         764  AGGTCCT              [GA]G^G[AT]CC[CT]
             3004  AGGACCT             
PshAI         138  GACATGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2700  GACCATGGTC          
SspI         1730  AATATT               AAT^ATT
             2339  AATATT              
StuI          522  AGGCCT               AGG^CCT
             3202  AGGCCT              
XcmI         1141  CCAAAGAACAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2978  CCATGGGGTTGATGG     
XmnI          800  GAATCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3934  GAAGATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CelII       Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII