################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AviII AvrII BbrPI BcgI BciVI BclI BlnI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BsrBI BsrDI BsrGI BssHII BstBI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MluI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SphI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1326 GGTACC G^GTACC AccII 100 CGCG CG^CG AccIII 539 TCCGGA T^CCGGA AclI 4346 AACGTT AA^CGTT AcyI 572 GGCGCC G[GA]^CG[CT]C AflII 957 CTTAAG C^TTAAG Alw44I 2040 GTGCAC G^TGCAC ApaLI 2040 GTGCAC G^TGCAC Asp718I 1326 GGTACC G^GTACC AspI 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 92 CCCGGG C^[CT]CG[GA]G BbeI 572 GGCGCC GGCGC^C BbvCI 1703 GCTGAGG CCTCAGC(-5/-2) BfrI 957 CTTAAG C^TTAAG BglII 3936 AGATCT A^GATCT BsaAI 4087 TACGTA [CT]AC^GT[GA] BsaHI 572 GGCGCC G[GA]^CG[CT]C BseAI 539 TCCGGA T^CCGGA BsoBI 92 CCCGGG C^[CT]CG[GA]G BspEI 539 TCCGGA T^CCGGA BsrFI 2375 GCCGGC [GA]^CCGG[CT] BssSI 433 CTCGTG CACGAG(-5/-1) Bst1107I 4276 GTATAC GTA^TAC BstEII 1836 GGTGACC G^GT[ACGT]ACC BstPI 1836 GGTGACC G^GT[ACGT]ACC BstUI 100 CGCG CG^CG BstZ17I 4276 GTATAC GTA^TAC Cfr10I 2375 GCCGGC [GA]^CCGG[CT] DraIII 1000 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1836 GGTGACC G^GT[ACGT]ACC EcoT22I 3449 ATGCAT ATGCA^T HaeII 572 GGCGCC [GA]GCGC^[CT] Hin1I 572 GGCGCC G[GA]^CG[CT]C KasI 572 GGCGCC G^GCGCC KpnI 1326 GGTACC GGTAC^C MroI 539 TCCGGA T^CCGGA MvnI 100 CGCG CG^CG NaeI 2375 GCCGGC GCC^GGC NarI 572 GGCGCC GG^CGCC NgoMIV 2375 GCCGGC G^CCGGC NsiI 3449 ATGCAT ATGCA^T PflFI 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 4346 AACGTT AA^CGTT SacI 1934 GAGCTC GAGCT^C SapI 3014 GAAGAGC GCTCTTC(1/4) SexAI 369 ACCTGGT A^CC[AT]GGT SfoI 572 GGCGCC GGC^GCC SmaI 92 CCCGGG CCC^GGG SnaBI 4087 TACGTA TAC^GTA SpeI 2631 ACTAGT A^CTAGT Tth111I 559 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 92 CCCGGG C^CCGGG XmaI 92 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 3297 GTAGAC GT^[AC][GT]AC 4276 GTATAC AflIII 2093 ACATGT A^C[GA][CT]GT 4335 ACATGT AlwNI 1917 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 3962 CAGCCACTG AocI 618 CCTGAGG CC^T[ACGT]AGG 1956 CCTTAGG Asp700I 800 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3934 GAAGATCTTC BalI 1153 TGGCCA TGG^CCA 3775 TGGCCA BamHI 31 GGATCC G^GATCC 4022 GGATCC BglI 1043 GCCCCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3232 GCCAACTGGGC BsaBI 3931 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4467 GATGTCTATC BsaI 842 GGTCTC GGTCTC(1/5) 4233 GGTCTC BsaWI 539 TCCGGA [AT]^CCGG[AT] 2934 ACCGGA BsmBI 387 CGTCTC CGTCTC(1/5) 4121 GAGACG BspLU11I 2093 ACATGT A^CATGT 4335 ACATGT Bsu36I 618 CCTGAGG CC^T[ACGT]AGG 1956 CCTTAGG CfoI 101 GCGC GCG^C 573 GCGC EaeI 1153 TGGCCA [CT]^GGCC[GA] 3775 TGGCCA Eco81I 618 CCTGAGG CC^T[ACGT]AGG 1956 CCTTAGG EcoRV 318 GATATC GAT^ATC 1482 GATATC HhaI 101 GCGC GCG^C 573 GCGC HinP1I 101 GCGC G^CGC 573 GCGC MamI 3931 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4467 GATGTCTATC MfeI 2028 CAATTG C^AATTG 2751 CAATTG MluNI 1153 TGGCCA TGG^CCA 3775 TGGCCA MscI 1153 TGGCCA TGG^CCA 3775 TGGCCA MunI 2028 CAATTG C^AATTG 2751 CAATTG PpuMI 764 AGGTCCT [GA]G^G[AT]CC[CT] 3004 AGGACCT PshAI 138 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2700 GACCATGGTC SspI 1730 AATATT AAT^ATT 2339 AATATT StuI 522 AGGCCT AGG^CCT 3202 AGGCCT XcmI 1141 CCAAAGAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2978 CCATGGGGTTGATGG XmnI 800 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3934 GAAGATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII