##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AhdI AocI
ApaI AscI Asp718I AspEI AspI
BamHI BbeI BcgI BglI BseAI
BsiWI BsmBI BsmI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT22I
FseI HpaI KasI KpnI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PpuMI PshAI Psp1406I RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2201 GACGTC GACGT^C
AgeI 3297 ACCGGT A^CCGGT
AlwNI 2348 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2081 AGCGCT AGC^GCT
AseI 3506 ATTAAT AT^TAAT
AsnI 3506 ATTAAT AT^TAAT
AvaI 2149 CTCGAG C^[CT]CG[GA]G
AviII 4210 TGCGCA TGC^GCA
BalI 1556 TGGCCA TGG^CCA
BanI 3027 GGTGCC G^G[CT][GA]CC
BanII 4425 GAGCTC G[GA]GC[CT]^C
BbvCI 3183 GCTGAGG CCTCAGC(-5/-2)
BciVI 2448 GTATCC GTATCC(6/5)
BlpI 3200 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3200 GCTGAGC GC^T[ACGT]AGC
BsaWI 3297 ACCGGT [AT]^CCGG[AT]
BsiEI 1345 CGATCG CG[GA][CT]^CG
BsmFI 1178 GGGAC GGGAC(10/14)
BsoBI 2149 CTCGAG C^[CT]CG[GA]G
BspDI 1343 ATCGAT AT^CGAT
BspLU11I 1956 ACATGT A^CATGT
BsrFI 3297 ACCGGT [GA]^CCGG[CT]
BstAPI 3120 GCATACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3039 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3200 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3297 ACCGGT [GA]^CCGG[CT]
ClaI 1343 ATCGAT AT^CGAT
Eco47III 2081 AGCGCT AGC^GCT
EcoRV 93 GATATC GAT^ATC
FspI 4210 TGCGCA TGC^GCA
HaeII 2081 AGCGCT [GA]GCGC^[CT]
KspI 23 CCGCGG CCGC^GG
MluNI 1556 TGGCCA TGG^CCA
MscI 1556 TGGCCA TGG^CCA
NcoI 2212 CCATGG C^CATGG
PaeR7I 2149 CTCGAG C^TCGAG
PflMI 1455 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3297 ACCGGT A^CCGGT
PshBI 3506 ATTAAT AT^TAAT
PvuI 1345 CGATCG CGAT^CG
SacI 4425 GAGCTC GAGCT^C
SacII 23 CCGCGG CCGC^GG
SphI 2531 GCATGC GCATG^C
Van91I 1455 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2149 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 111 GTAGAC GT^[AC][GT]AC
4005 GTAGAC
AccII 24 CGCG CG^CG
4238 CGCG
AcyI 72 GGCGTC G[GA]^CG[CT]C
2201 GACGTC
Alw44I 802 GTGCAC G^TGCAC
2111 GTGCAC
ApaLI 802 GTGCAC G^TGCAC
2111 GTGCAC
AvaII 1133 GGACC G^G[AT]CC
1179 GGACC
AvrII 1218 CCTAGG C^CTAGG
3585 CCTAGG
BbrPI 2159 CACGTG CAC^GTG
3950 CACGTG
BbsI 4328 GAAGAC GAAGAC(2/6)
653 GTCTTC
BclI 257 TGATCA T^GATCA
932 TGATCA
BlnI 1218 CCTAGG C^CTAGG
3585 CCTAGG
BpmI 886 CTCCAG CTGGAG(16/14)
3037 CTCCAG
BpuAI 4328 GAAGAC GAAGAC(2/6)
653 GTCTTC
BsaBI 1083 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3963 GATCTGCATC
BsaHI 72 GGCGTC G[GA]^CG[CT]C
2201 GACGTC
BsaI 1608 GGTCTC GGTCTC(1/5)
3132 GGTCTC
BsgI 3095 CTGCAC GTGCAG(16/14)
3437 CTGCAC
Bsp1407I 3398 TGTACA T^GTACA
4405 TGTACA
BspMI 3063 ACCTGC ACCTGC(4/8)
3989 GCAGGT
BsrGI 3398 TGTACA T^GTACA
4405 TGTACA
BstUI 24 CGCG CG^CG
4238 CGCG
DraIII 2322 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2578 CACTGTGTG
EcoRI 502 GAATTC G^AATTC
2715 GAATTC
FauI 25 GCGGG CCCGC(4/6)
3985 GCGGG
FbaI 257 TGATCA T^GATCA
932 TGATCA
HgaI 4331 GACGC GACGC(5/10)
73 GCGTC
Hin1I 72 GGCGTC G[GA]^CG[CT]C
2201 GACGTC
HindIII 1153 AAGCTT A^AGCTT
3684 AAGCTT
MamI 1083 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3963 GATCTGCATC
MvnI 24 CGCG CG^CG
4238 CGCG
NlaIV 1178 GGGACC GG[ACGT]^[ACGT]CC
3027 GGTGCC
PmaCI 2159 CACGTG CAC^GTG
3950 CACGTG
PmlI 2159 CACGTG CAC^GTG
3950 CACGTG
PvuII 1482 CAGCTG CAG^CTG
1613 CAGCTG
SspBI 3398 TGTACA T^GTACA
4405 TGTACA
XbaI 1091 TCTAGA T^CTAGA
2643 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I AspHI
BbvI BcnI BfaI BfrI BglII
BmyI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NspI PleI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI