################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I BamHI BbeI BbrPI BcgI BciVI BfrI BsaAI BsaBI BsiWI BspDI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV EcoT22I FseI HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII ScaI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccIII 229 TCCGGA T^CCGGA AcsI 1134 GAATTC [GA]^AATT[CT] AocI 584 CCTCAGG CC^T[ACGT]AGG Aor51HI 2938 AGCGCT AGC^GCT ApoI 1134 GAATTC [GA]^AATT[CT] AvrII 4730 CCTAGG C^CTAGG BlnI 4730 CCTAGG C^CTAGG BseAI 229 TCCGGA T^CCGGA BsiEI 3374 CGATCG CG[GA][CT]^CG BsmI 3458 GAATGC GAATGC(1/-1) Bsp1407I 73 TGTACA T^GTACA BspEI 229 TCCGGA T^CCGGA BsrGI 73 TGTACA T^GTACA BssHII 3098 GCGCGC G^CGCGC Bsu36I 584 CCTCAGG CC^T[ACGT]AGG DraI 5044 TTTAAA TTT^AAA DraIII 183 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2938 AGCGCT AGC^GCT Eco81I 584 CCTCAGG CC^T[ACGT]AGG EcoRI 1134 GAATTC G^AATTC MroI 229 TCCGGA T^CCGGA NaeI 165 GCCGGC GCC^GGC NgoMIV 165 GCCGGC G^CCGGC NheI 428 GCTAGC G^CTAGC PvuI 3374 CGATCG CGAT^CG SalI 3966 GTCGAC G^TCGAC SexAI 23 ACCTGGT A^CC[AT]GGT SphI 3076 GCATGC GCATG^C SspBI 73 TGTACA T^GTACA XbaI 4592 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 3966 GTCGAC GT^[AC][GT]AC 4923 GTCTAC AcyI 213 GGCGTC G[GA]^CG[CT]C 3728 GGCGTC AspI 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4096 GACCATGTC AviII 592 TGCGCA TGC^GCA 4947 TGCGCA BglII 871 AGATCT A^GATCT 1462 AGATCT BsaHI 213 GGCGTC G[GA]^CG[CT]C 3728 GGCGTC BsaWI 229 TCCGGA [AT]^CCGG[AT] 4612 TCCGGT BsgI 4953 CTGCAC GTGCAG(16/14) 4491 GTGCAG BsmBI 2432 CGTCTC CGTCTC(1/5) 741 GAGACG BsrFI 165 GCCGGC [GA]^CCGG[CT] 2089 ACCGGC BssSI 1200 CACGAG CACGAG(-5/-1) 2688 CACGAG Cfr10I 165 GCCGGC [GA]^CCGG[CT] 2089 ACCGGC DrdI 743 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3957 GACGAGGAGGTC FspI 592 TGCGCA TGC^GCA 4947 TGCGCA Hin1I 213 GGCGTC G[GA]^CG[CT]C 3728 GGCGTC HincII 2406 GTTGAC GT[CT]^[GA]AC 3966 GTCGAC HindII 2406 GTTGAC GT[CT]^[GA]AC 3966 GTCGAC HindIII 2019 AAGCTT A^AGCTT 3051 AAGCTT PflFI 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4096 GACCATGTC SbfI 926 CCTGCAGG CCTGCA^GG 2810 CCTGCAGG SmaI 2572 CCCGGG CCC^GGG 3106 CCCGGG Sse8387I 926 CCTGCAGG CCTGCA^GG 2810 CCTGCAGG Tsp509I 1135 AATT ^AATT 3926 AATT Tth111I 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4096 GACCATGTC XmaCI 2572 CCCGGG C^CCGGG 3106 CCCGGG XmaI 2572 CCCGGG C^CCGGG 3106 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AfaI AhdI AluI AlwI AlwNI ApaI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmnI