##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I BamHI BbeI BbrPI
BcgI BciVI BfrI BsaAI BsaBI
BsiWI BspDI BspLU11I Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoRV EcoT22I FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RsrII SacII ScaI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 229 TCCGGA T^CCGGA
AcsI 1134 GAATTC [GA]^AATT[CT]
AocI 584 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2938 AGCGCT AGC^GCT
ApoI 1134 GAATTC [GA]^AATT[CT]
AvrII 4730 CCTAGG C^CTAGG
BlnI 4730 CCTAGG C^CTAGG
BseAI 229 TCCGGA T^CCGGA
BsiEI 3374 CGATCG CG[GA][CT]^CG
BsmI 3458 GAATGC GAATGC(1/-1)
Bsp1407I 73 TGTACA T^GTACA
BspEI 229 TCCGGA T^CCGGA
BsrGI 73 TGTACA T^GTACA
BssHII 3098 GCGCGC G^CGCGC
Bsu36I 584 CCTCAGG CC^T[ACGT]AGG
DraI 5044 TTTAAA TTT^AAA
DraIII 183 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2938 AGCGCT AGC^GCT
Eco81I 584 CCTCAGG CC^T[ACGT]AGG
EcoRI 1134 GAATTC G^AATTC
MroI 229 TCCGGA T^CCGGA
NaeI 165 GCCGGC GCC^GGC
NgoMIV 165 GCCGGC G^CCGGC
NheI 428 GCTAGC G^CTAGC
PvuI 3374 CGATCG CGAT^CG
SalI 3966 GTCGAC G^TCGAC
SexAI 23 ACCTGGT A^CC[AT]GGT
SphI 3076 GCATGC GCATG^C
SspBI 73 TGTACA T^GTACA
XbaI 4592 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3966 GTCGAC GT^[AC][GT]AC
4923 GTCTAC
AcyI 213 GGCGTC G[GA]^CG[CT]C
3728 GGCGTC
AspI 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4096 GACCATGTC
AviII 592 TGCGCA TGC^GCA
4947 TGCGCA
BglII 871 AGATCT A^GATCT
1462 AGATCT
BsaHI 213 GGCGTC G[GA]^CG[CT]C
3728 GGCGTC
BsaWI 229 TCCGGA [AT]^CCGG[AT]
4612 TCCGGT
BsgI 4953 CTGCAC GTGCAG(16/14)
4491 GTGCAG
BsmBI 2432 CGTCTC CGTCTC(1/5)
741 GAGACG
BsrFI 165 GCCGGC [GA]^CCGG[CT]
2089 ACCGGC
BssSI 1200 CACGAG CACGAG(-5/-1)
2688 CACGAG
Cfr10I 165 GCCGGC [GA]^CCGG[CT]
2089 ACCGGC
DrdI 743 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3957 GACGAGGAGGTC
FspI 592 TGCGCA TGC^GCA
4947 TGCGCA
Hin1I 213 GGCGTC G[GA]^CG[CT]C
3728 GGCGTC
HincII 2406 GTTGAC GT[CT]^[GA]AC
3966 GTCGAC
HindII 2406 GTTGAC GT[CT]^[GA]AC
3966 GTCGAC
HindIII 2019 AAGCTT A^AGCTT
3051 AAGCTT
PflFI 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4096 GACCATGTC
SbfI 926 CCTGCAGG CCTGCA^GG
2810 CCTGCAGG
SmaI 2572 CCCGGG CCC^GGG
3106 CCCGGG
Sse8387I 926 CCTGCAGG CCTGCA^GG
2810 CCTGCAGG
Tsp509I 1135 AATT ^AATT
3926 AATT
Tth111I 1944 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4096 GACCATGTC
XmaCI 2572 CCCGGG C^CCGGG
3106 CCCGGG
XmaI 2572 CCCGGG C^CCGGG
3106 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI AlwNI ApaI Asp700I AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmnI