##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     BamHI       BbeI        BbrPI       
BcgI        BciVI       BfrI        BsaAI       BsaBI       
BsiWI       BspDI       BspLU11I    Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        EagI        EclXI       
Eco52I      EcoRV       EcoT22I     FseI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MunI        NarI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    RsrII       SacII       ScaI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccIII        229  TCCGGA               T^CCGGA
AcsI         1134  GAATTC               [GA]^AATT[CT]
AocI          584  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2938  AGCGCT               AGC^GCT
ApoI         1134  GAATTC               [GA]^AATT[CT]
AvrII        4730  CCTAGG               C^CTAGG
BlnI         4730  CCTAGG               C^CTAGG
BseAI         229  TCCGGA               T^CCGGA
BsiEI        3374  CGATCG               CG[GA][CT]^CG
BsmI         3458  GAATGC               GAATGC(1/-1)
Bsp1407I       73  TGTACA               T^GTACA
BspEI         229  TCCGGA               T^CCGGA
BsrGI          73  TGTACA               T^GTACA
BssHII       3098  GCGCGC               G^CGCGC
Bsu36I        584  CCTCAGG              CC^T[ACGT]AGG
DraI         5044  TTTAAA               TTT^AAA
DraIII        183  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2938  AGCGCT               AGC^GCT
Eco81I        584  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1134  GAATTC               G^AATTC
MroI          229  TCCGGA               T^CCGGA
NaeI          165  GCCGGC               GCC^GGC
NgoMIV        165  GCCGGC               G^CCGGC
NheI          428  GCTAGC               G^CTAGC
PvuI         3374  CGATCG               CGAT^CG
SalI         3966  GTCGAC               G^TCGAC
SexAI          23  ACCTGGT              A^CC[AT]GGT
SphI         3076  GCATGC               GCATG^C
SspBI          73  TGTACA               T^GTACA
XbaI         4592  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         3966  GTCGAC               GT^[AC][GT]AC
             4923  GTCTAC              
AcyI          213  GGCGTC               G[GA]^CG[CT]C
             3728  GGCGTC              
AspI         1944  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4096  GACCATGTC           
AviII         592  TGCGCA               TGC^GCA
             4947  TGCGCA              
BglII         871  AGATCT               A^GATCT
             1462  AGATCT              
BsaHI         213  GGCGTC               G[GA]^CG[CT]C
             3728  GGCGTC              
BsaWI         229  TCCGGA               [AT]^CCGG[AT]
             4612  TCCGGT              
BsgI         4953  CTGCAC               GTGCAG(16/14)
             4491  GTGCAG              
BsmBI        2432  CGTCTC               CGTCTC(1/5)
              741  GAGACG              
BsrFI         165  GCCGGC               [GA]^CCGG[CT]
             2089  ACCGGC              
BssSI        1200  CACGAG               CACGAG(-5/-1)
             2688  CACGAG              
Cfr10I        165  GCCGGC               [GA]^CCGG[CT]
             2089  ACCGGC              
DrdI          743  GACGATGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3957  GACGAGGAGGTC        
FspI          592  TGCGCA               TGC^GCA
             4947  TGCGCA              
Hin1I         213  GGCGTC               G[GA]^CG[CT]C
             3728  GGCGTC              
HincII       2406  GTTGAC               GT[CT]^[GA]AC
             3966  GTCGAC              
HindII       2406  GTTGAC               GT[CT]^[GA]AC
             3966  GTCGAC              
HindIII      2019  AAGCTT               A^AGCTT
             3051  AAGCTT              
PflFI        1944  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4096  GACCATGTC           
SbfI          926  CCTGCAGG             CCTGCA^GG
             2810  CCTGCAGG            
SmaI         2572  CCCGGG               CCC^GGG
             3106  CCCGGG              
Sse8387I      926  CCTGCAGG             CCTGCA^GG
             2810  CCTGCAGG            
Tsp509I      1135  AATT                 ^AATT
             3926  AATT                
Tth111I      1944  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4096  GACCATGTC           
XmaCI        2572  CCCGGG               C^CCGGG
             3106  CCCGGG              
XmaI         2572  CCCGGG               C^CCGGG
             3106  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AfaI        AhdI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspEI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BclI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BspMI       
BsrBI       BsrDI       BsrI        BssKI       BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmnI