##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BcgI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KspI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmeI
PshBI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 3740 CGCG CG^CG
AclI 4320 AACGTT AA^CGTT
Alw44I 590 GTGCAC G^TGCAC
ApaLI 590 GTGCAC G^TGCAC
Asp700I 1486 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2586 TGGCCA TGG^CCA
BbrPI 1018 CACGTG CAC^GTG
BbvCI 1799 CCTCAGC CCTCAGC(-5/-2)
BclI 126 TGATCA T^GATCA
BglI 1219 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2543 AGATCT A^GATCT
BlpI 1108 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1108 GCTCAGC GC^T[ACGT]AGC
BsaBI 3970 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2842 GAGACG CGTCTC(1/5)
BspHI 3652 TCATGA T^CATGA
BsrBI 3054 GAGCGG CCGCTC(-3/-3)
BstEII 829 GGTTACC G^GT[ACGT]ACC
BstPI 829 GGTTACC G^GT[ACGT]ACC
BstUI 3740 CGCG CG^CG
CelII 1108 GCTCAGC GC^T[ACGT]AGC
EaeI 2586 TGGCCA [CT]^GGCC[GA]
EcoNI 3638 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 829 GGTTACC G^GT[ACGT]ACC
EcoRI 2451 GAATTC G^AATTC
FauI 3056 GCGGG CCCGC(4/6)
FbaI 126 TGATCA T^GATCA
MamI 3970 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3739 ACGCGT A^CGCGT
MluNI 2586 TGGCCA TGG^CCA
MscI 2586 TGGCCA TGG^CCA
MvnI 3740 CGCG CG^CG
PacI 3878 TTAATTAA TTAAT^TAA
PmaCI 1018 CACGTG CAC^GTG
PmlI 1018 CACGTG CAC^GTG
PshAI 571 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4320 AACGTT AA^CGTT
RcaI 3652 TCATGA T^CATGA
SacI 3379 GAGCTC GAGCT^C
SpeI 3761 ACTAGT A^CTAGT
SspI 876 AATATT AAT^ATT
XbaI 3444 TCTAGA T^CTAGA
XmnI 1486 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 2498 GGTACC G^GTACC
4051 GGTACC
AflII 1760 CTTAAG C^TTAAG
3863 CTTAAG
ApaI 1479 GGGCCC GGGCC^C
1959 GGGCCC
Asp718I 2498 GGTACC G^GTACC
4051 GGTACC
BanI 2498 GGTACC G^G[CT][GA]CC
4051 GGTACC
BciVI 1591 GGATAC GTATCC(6/5)
2969 GTATCC
BfrI 1760 CTTAAG C^TTAAG
3863 CTTAAG
BspMI 884 ACCTGC ACCTGC(4/8)
2109 GCAGGT
BsrDI 4197 CATTGC GCAATG(2/0)
2554 GCAATG
BstYI 383 GGATCT [GA]^GATC[CT]
2543 AGATCT
EcoT22I 246 ATGCAT ATGCA^T
250 ATGCAT
KpnI 2498 GGTACC GGTAC^C
4051 GGTACC
MfeI 3794 CAATTG C^AATTG
4630 CAATTG
MflI 383 GGATCT [GA]^GATC[CT]
2543 AGATCT
MunI 3794 CAATTG C^AATTG
4630 CAATTG
NdeI 3690 CATATG CA^TATG
4618 CATATG
NsiI 246 ATGCAT ATGCA^T
250 ATGCAT
PflMI 1286 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1949 CCAGACAGTGG
PvuII 668 CAGCTG CAG^CTG
4672 CAGCTG
SfaNI 39 GCATC GCATC(5/9)
2489 GCATC
SphI 248 GCATGC GCATG^C
2752 GCATGC
TaqI 1346 TCGA T^CGA
2455 TCGA
TthHB8I 1346 TCGA T^CGA
2455 TCGA
Van91I 1286 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1949 CCAGACAGTGG
XhoII 383 GGATCT [GA]^GATC[CT]
2543 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI