##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccI AgeI Aor51HI
AseI AsnI Asp718I AviII BciVI
BsaBI BsaI BsiWI BsmBI BsmI
BspDI BsrBI BsrDI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HindIII KpnI MamI
MfeI MunI NcoI NdeI NheI
NruI NsiI PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RsrII
SacI SalI SbfI SfiI SnaBI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 1169 TCCGGA T^CCGGA
AclI 2819 AACGTT AA^CGTT
AflII 4027 CTTAAG C^TTAAG
AhdI 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3805 GTGCAC G^TGCAC
ApaLI 3805 GTGCAC G^TGCAC
AscI 413 GGCGCGCC GG^CGCGCC
AspEI 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3136 CCTAGG C^CTAGG
BalI 1560 TGGCCA TGG^CCA
BamHI 1954 GGATCC G^GATCC
BbeI 518 GGCGCC GGCGC^C
BbrPI 3389 CACGTG CAC^GTG
BbvCI 329 GCTGAGG CCTCAGC(-5/-2)
BcgI 428 GCATGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4027 CTTAAG C^TTAAG
BglI 372 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3136 CCTAGG C^CTAGG
BsaAI 3389 CACGTG [CT]AC^GT[GA]
BsaWI 1169 TCCGGA [AT]^CCGG[AT]
BseAI 1169 TCCGGA T^CCGGA
Bsp1407I 1130 TGTACA T^GTACA
BspEI 1169 TCCGGA T^CCGGA
BspLU11I 2027 ACATGT A^CATGT
BsrGI 1130 TGTACA T^GTACA
BstAPI 2190 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2969 TTCGAA TT^CGAA
DraI 2845 TTTAAA TTT^AAA
Eam1105I 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 150 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 3193 GTTAAC GT[CT]^[GA]AC
HindII 3193 GTTAAC GT[CT]^[GA]AC
HpaI 3193 GTTAAC GTT^AAC
KasI 518 GGCGCC G^GCGCC
MluI 1802 ACGCGT A^CGCGT
MluNI 1560 TGGCCA TGG^CCA
MroI 1169 TCCGGA T^CCGGA
MscI 1560 TGGCCA TGG^CCA
NarI 518 GGCGCC GG^CGCC
NotI 257 GCGGCCGC GC^GGCCGC
NspV 2969 TTCGAA TT^CGAA
PflFI 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1102 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3389 CACGTG CAC^GTG
PmlI 3389 CACGTG CAC^GTG
Psp1406I 2819 AACGTT AA^CGTT
ScaI 3779 AGTACT AGT^ACT
SexAI 2291 ACCTGGT A^CC[AT]GGT
SfoI 518 GGCGCC GGC^GCC
SfuI 2969 TTCGAA TT^CGAA
SgrAI 200 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 3793 ACTAGT A^CTAGT
SphI 428 GCATGC GCATG^C
SspBI 1130 TGTACA T^GTACA
SspI 3826 AATATT AAT^ATT
StuI 1601 AGGCCT AGG^CCT
Tth111I 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1102 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 2191 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2716 CAGCAGCTG
ApaI 248 GGGCCC GGGCC^C
507 GGGCCC
BlpI 423 GCTCAGC GC^T[ACGT]AGC
1350 GCTCAGC
Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC
1350 GCTCAGC
BspHI 665 TCATGA T^CATGA
842 TCATGA
BssSI 1846 CACGAG CACGAG(-5/-1)
442 CTCGTG
CelII 423 GCTCAGC GC^T[ACGT]AGC
1350 GCTCAGC
DrdI 2185 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2700 GACATTTGGGTC
PpuMI 1897 GGGTCCC [GA]G^G[AT]CC[CT]
2192 AGGTCCT
RcaI 665 TCATGA T^CATGA
842 TCATGA
SapI 2243 GCTCTTC GCTCTTC(1/4)
2271 GCTCTTC
SmaI 272 CCCGGG CCC^GGG
733 CCCGGG
XmaCI 272 CCCGGG C^CCGGG
733 CCCGGG
XmaI 272 CCCGGG C^CCGGG
733 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI