################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII Acc65I AccI AgeI Aor51HI AseI AsnI Asp718I AviII BciVI BsaBI BsaI BsiWI BsmBI BsmI BspDI BsrBI BsrDI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII KpnI MamI MfeI MunI NcoI NdeI NheI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RsrII SacI SalI SbfI SfiI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 1169 TCCGGA T^CCGGA AclI 2819 AACGTT AA^CGTT AflII 4027 CTTAAG C^TTAAG AhdI 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3805 GTGCAC G^TGCAC ApaLI 3805 GTGCAC G^TGCAC AscI 413 GGCGCGCC GG^CGCGCC AspEI 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3136 CCTAGG C^CTAGG BalI 1560 TGGCCA TGG^CCA BamHI 1954 GGATCC G^GATCC BbeI 518 GGCGCC GGCGC^C BbrPI 3389 CACGTG CAC^GTG BbvCI 329 GCTGAGG CCTCAGC(-5/-2) BcgI 428 GCATGCTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4027 CTTAAG C^TTAAG BglI 372 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3136 CCTAGG C^CTAGG BsaAI 3389 CACGTG [CT]AC^GT[GA] BsaWI 1169 TCCGGA [AT]^CCGG[AT] BseAI 1169 TCCGGA T^CCGGA Bsp1407I 1130 TGTACA T^GTACA BspEI 1169 TCCGGA T^CCGGA BspLU11I 2027 ACATGT A^CATGT BsrGI 1130 TGTACA T^GTACA BstAPI 2190 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2969 TTCGAA TT^CGAA DraI 2845 TTTAAA TTT^AAA Eam1105I 1891 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 150 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 3193 GTTAAC GT[CT]^[GA]AC HindII 3193 GTTAAC GT[CT]^[GA]AC HpaI 3193 GTTAAC GTT^AAC KasI 518 GGCGCC G^GCGCC MluI 1802 ACGCGT A^CGCGT MluNI 1560 TGGCCA TGG^CCA MroI 1169 TCCGGA T^CCGGA MscI 1560 TGGCCA TGG^CCA NarI 518 GGCGCC GG^CGCC NotI 257 GCGGCCGC GC^GGCCGC NspV 2969 TTCGAA TT^CGAA PflFI 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1102 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3389 CACGTG CAC^GTG PmlI 3389 CACGTG CAC^GTG Psp1406I 2819 AACGTT AA^CGTT ScaI 3779 AGTACT AGT^ACT SexAI 2291 ACCTGGT A^CC[AT]GGT SfoI 518 GGCGCC GGC^GCC SfuI 2969 TTCGAA TT^CGAA SgrAI 200 CGCCGGCG C[GA]^CCGG[CT]G SpeI 3793 ACTAGT A^CTAGT SphI 428 GCATGC GCATG^C SspBI 1130 TGTACA T^GTACA SspI 3826 AATATT AAT^ATT StuI 1601 AGGCCT AGG^CCT Tth111I 1612 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1102 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwNI 2191 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 2716 CAGCAGCTG ApaI 248 GGGCCC GGGCC^C 507 GGGCCC BlpI 423 GCTCAGC GC^T[ACGT]AGC 1350 GCTCAGC Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC 1350 GCTCAGC BspHI 665 TCATGA T^CATGA 842 TCATGA BssSI 1846 CACGAG CACGAG(-5/-1) 442 CTCGTG CelII 423 GCTCAGC GC^T[ACGT]AGC 1350 GCTCAGC DrdI 2185 GACATGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2700 GACATTTGGGTC PpuMI 1897 GGGTCCC [GA]G^G[AT]CC[CT] 2192 AGGTCCT RcaI 665 TCATGA T^CATGA 842 TCATGA SapI 2243 GCTCTTC GCTCTTC(1/4) 2271 GCTCTTC SmaI 272 CCCGGG CCC^GGG 733 CCCGGG XmaCI 272 CCCGGG C^CCGGG 733 CCCGGG XmaI 272 CCCGGG C^CCGGG 733 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI