##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI BbrPI BfrI BsaAI BsaBI
BsaI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I MamI MluI NdeI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
RsrII SalI SapI SexAI SfiI
SfuI SnaBI SphI SspBI StuI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1389 GACGTC GACGT^C
Acc65I 911 GGTACC G^GTACC
AccI 4016 GTCTAC GT^[AC][GT]AC
AclI 1545 AACGTT AA^CGTT
AflIII 234 ACGTGT A^C[GA][CT]GT
AocI 3448 CCTTAGG CC^T[ACGT]AGG
ApaI 465 GGGCCC GGGCC^C
Asp718I 911 GGTACC G^GTACC
BbvCI 1512 CCTCAGC CCTCAGC(-5/-2)
BciVI 2407 GTATCC GTATCC(6/5)
BclI 1552 TGATCA T^GATCA
BssHII 387 GCGCGC G^CGCGC
BssSI 768 CACGAG CACGAG(-5/-1)
Bsu36I 3448 CCTTAGG CC^T[ACGT]AGG
DraI 1938 TTTAAA TTT^AAA
DraIII 4762 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3448 CCTTAGG CC^T[ACGT]AGG
EcoRI 1965 GAATTC G^AATTC
FbaI 1552 TGATCA T^GATCA
FseI 561 GGCCGGCC GGCCGG^CC
HpaI 1887 GTTAAC GTT^AAC
KpnI 911 GGTACC GGTAC^C
MfeI 3048 CAATTG C^AATTG
MunI 3048 CAATTG C^AATTG
NheI 3168 GCTAGC G^CTAGC
NruI 505 TCGCGA TCG^CGA
PaeR7I 1685 CTCGAG C^TCGAG
Psp1406I 1545 AACGTT AA^CGTT
SbfI 2802 CCTGCAGG CCTGCA^GG
Sse8387I 2802 CCTGCAGG CCTGCA^GG
SspI 2672 AATATT AAT^ATT
XhoI 1685 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 738 TCCGGA T^CCGGA
3782 TCCGGA
AviII 2588 TGCGCA TGC^GCA
4919 TGCGCA
AvrII 3912 CCTAGG C^CTAGG
4523 CCTAGG
BamHI 372 GGATCC G^GATCC
2761 GGATCC
BlnI 3912 CCTAGG C^CTAGG
4523 CCTAGG
BlpI 595 GCTGAGC GC^T[ACGT]AGC
1194 GCTCAGC
Bpu1102I 595 GCTGAGC GC^T[ACGT]AGC
1194 GCTCAGC
BsaWI 738 TCCGGA [AT]^CCGG[AT]
3782 TCCGGA
BseAI 738 TCCGGA T^CCGGA
3782 TCCGGA
BsmI 1562 GAATGC GAATGC(1/-1)
3217 GAATGC
BspEI 738 TCCGGA T^CCGGA
3782 TCCGGA
BstAPI 10 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3528 GCAGCCCCTGC
CelII 595 GCTGAGC GC^T[ACGT]AGC
1194 GCTCAGC
FspI 2588 TGCGCA TGC^GCA
4919 TGCGCA
HincII 1887 GTTAAC GT[CT]^[GA]AC
2887 GTCAAC
HindII 1887 GTTAAC GT[CT]^[GA]AC
2887 GTCAAC
HindIII 4080 AAGCTT A^AGCTT
4430 AAGCTT
MroI 738 TCCGGA T^CCGGA
3782 TCCGGA
MslI 4140 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4632 CATTGAAATG
NotI 615 GCGGCCGC GC^GGCCGC
954 GCGGCCGC
NspI 1643 ACATGC [GA]CATG^[CT]
4922 GCATGT
PflMI 1764 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2601 CCAGGCCATGG
PshAI 4416 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4818 GACCGATGTC
ScaI 1812 AGTACT AGT^ACT
4448 AGTACT
SgrAI 210 CGCCGGCG C[GA]^CCGG[CT]G
610 CGCCGGCG
SmaI 204 CCCGGG CCC^GGG
462 CCCGGG
SmlI 1685 CTCGAG C^T[CT][GA]AG
2332 CTCAAG
SpeI 2079 ACTAGT A^CTAGT
4103 ACTAGT
Van91I 1764 CCATCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2601 CCAGGCCATGG
XmaCI 204 CCCGGG C^CCGGG
462 CCCGGG
XmaI 204 CCCGGG C^CCGGG
462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI