##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I BciVI BclI BfrI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrDI BssHII BstBI
BstEII BstPI ClaI CpoI DrdI
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI KpnI KspI MfeI MluI
MroI MunI NheI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1964 GACGTC GACGT^C
AccI 2515 GTATAC GT^[AC][GT]AC
AcsI 1496 GAATTT [GA]^AATT[CT]
Aor51HI 1402 AGCGCT AGC^GCT
ApoI 1496 GAATTT [GA]^AATT[CT]
BbeI 3452 GGCGCC GGCGC^C
BbrPI 1761 CACGTG CAC^GTG
BcgI 3605 GCAAAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1761 CACGTG [CT]AC^GT[GA]
BsaBI 1772 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1579 TCCGGT [AT]^CCGG[AT]
BsiEI 1338 CGGCCG CG[GA][CT]^CG
Bsp1407I 11 TGTACA T^GTACA
BsrGI 11 TGTACA T^GTACA
Bst1107I 2515 GTATAC GTA^TAC
BstAPI 3748 GCAACCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2515 GTATAC GTA^TAC
DraI 1873 TTTAAA TTT^AAA
DraIII 4199 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1338 CGGCCG C^GGCCG
EclXI 1338 CGGCCG C^GGCCG
Eco47III 1402 AGCGCT AGC^GCT
Eco52I 1338 CGGCCG C^GGCCG
HindIII 4462 AAGCTT A^AGCTT
HpaI 3035 GTTAAC GTT^AAC
KasI 3452 GGCGCC G^GCGCC
MamI 1772 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2345 GCCGGC GCC^GGC
NarI 3452 GGCGCC GG^CGCC
NdeI 1641 CATATG CA^TATG
NgoMIV 2345 GCCGGC G^CCGGC
NotI 1337 GCGGCCGC GC^GGCCGC
PaeR7I 2378 CTCGAG C^TCGAG
PmaCI 1761 CACGTG CAC^GTG
PmlI 1761 CACGTG CAC^GTG
PshAI 4610 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2994 GAGCTC GAGCT^C
ScaI 2254 AGTACT AGT^ACT
SfiI 3891 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3452 GGCGCC GGC^GCC
SspBI 11 TGTACA T^GTACA
XhoI 2378 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 3231 CGCG CG^CG
3359 CGCG
AhdI 821 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4207 GACCAAATGTC
AspEI 821 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4207 GACCAAATGTC
AspI 4159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4432 GACCCAGTC
AviII 440 TGCGCA TGC^GCA
2959 TGCGCA
BamHI 1263 GGATCC G^GATCC
1510 GGATCC
BbvCI 1398 CCTCAGC CCTCAGC(-5/-2)
960 GCTGAGG
BglII 74 AGATCT A^GATCT
4028 AGATCT
BsmI 1975 GAATGC GAATGC(1/-1)
2065 GCATTC
BsrFI 2345 GCCGGC [GA]^CCGG[CT]
2657 GCCGGT
BstUI 3231 CGCG CG^CG
3359 CGCG
Cfr10I 2345 GCCGGC [GA]^CCGG[CT]
2657 GCCGGT
Eam1105I 821 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4207 GACCAAATGTC
FspI 440 TGCGCA TGC^GCA
2959 TGCGCA
HgaI 1107 GCGTC GACGC(5/10)
3535 GCGTC
HincII 2445 GTTGAC GT[CT]^[GA]AC
3035 GTTAAC
HindII 2445 GTTGAC GT[CT]^[GA]AC
3035 GTTAAC
MaeII 1762 ACGT A^CGT
1965 ACGT
MvnI 3231 CGCG CG^CG
3359 CGCG
PflFI 4159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4432 GACCCAGTC
PflMI 1538 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2870 CCATGACCTGG
SmaI 2723 CCCGGG CCC^GGG
3763 CCCGGG
SphI 2962 GCATGC GCATG^C
4689 GCATGC
StuI 1785 AGGCCT AGG^CCT
4633 AGGCCT
TfiI 2262 GAATC G^A[AT]TC
3975 GAATC
Tth111I 4159 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4432 GACCCAGTC
Van91I 1538 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2870 CCATGACCTGG
XmaCI 2723 CCCGGG C^CCGGG
3763 CCCGGG
XmaI 2723 CCCGGG C^CCGGG
3763 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI Asp700I AspHI
AvaI AvaII AvrII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI