##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI BcgI BfrI
BglI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I EcoRV HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2621 GACGTC GACGT^C
AccI 3553 GTCTAC GT^[AC][GT]AC
Asp700I 4284 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1097 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3939 CCTAGG C^CTAGG
BamHI 3279 GGATCC G^GATCC
BbeI 1768 GGCGCC GGCGC^C
BbrPI 2555 CACGTG CAC^GTG
BciVI 3582 GTATCC GTATCC(6/5)
BglII 1107 AGATCT A^GATCT
BlnI 3939 CCTAGG C^CTAGG
BsaBI 2875 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1082 TGTACA T^GTACA
BsrGI 1082 TGTACA T^GTACA
BssSI 1195 CACGAG CACGAG(-5/-1)
BstEII 986 GGTTACC G^GT[ACGT]ACC
BstPI 986 GGTTACC G^GT[ACGT]ACC
DraI 3461 TTTAAA TTT^AAA
DraIII 1075 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 986 GGTTACC G^GT[ACGT]ACC
EcoRI 233 GAATTC G^AATTC
EcoT22I 2659 ATGCAT ATGCA^T
FseI 3206 GGCCGGCC GGCCGG^CC
HincII 2954 GTCAAC GT[CT]^[GA]AC
HindII 2954 GTCAAC GT[CT]^[GA]AC
KasI 1768 GGCGCC G^GCGCC
MamI 2875 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1768 GGCGCC GG^CGCC
NheI 3409 GCTAGC G^CTAGC
NsiI 2659 ATGCAT ATGCA^T
PflFI 1097 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2555 CACGTG CAC^GTG
PmlI 2555 CACGTG CAC^GTG
SapI 3358 GCTCTTC GCTCTTC(1/4)
SfoI 1768 GGCGCC GGC^GCC
SmaI 118 CCCGGG CCC^GGG
SphI 2755 GCATGC GCATG^C
SspBI 1082 TGTACA T^GTACA
Tth111I 1097 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 118 CCCGGG C^CCGGG
XmaI 118 CCCGGG C^CCGGG
XmnI 4284 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 233 GAATTC [GA]^AATT[CT]
4083 AAATTC
Aor51HI 2521 AGCGCT AGC^GCT
4351 AGCGCT
ApaI 1708 GGGCCC GGGCC^C
3997 GGGCCC
ApoI 233 GAATTC [GA]^AATT[CT]
4083 AAATTC
AvaI 118 CCCGGG C^[CT]CG[GA]G
1470 CCCGAG
BbsI 709 GAAGAC GAAGAC(2/6)
1597 GAAGAC
BbvCI 1316 GCTGAGG CCTCAGC(-5/-2)
3793 GCTGAGG
BclI 969 TGATCA T^GATCA
3559 TGATCA
BpuAI 709 GAAGAC GAAGAC(2/6)
1597 GAAGAC
BsaAI 1820 TACGTG [CT]AC^GT[GA]
2555 CACGTG
BsaWI 802 ACCGGA [AT]^CCGG[AT]
1368 ACCGGA
BsiEI 82 CGGCCG CG[GA][CT]^CG
4060 CGGCCG
BsmI 653 GAATGC GAATGC(1/-1)
2492 GAATGC
BsoBI 118 CCCGGG C^[CT]CG[GA]G
1470 CCCGAG
BspHI 207 TCATGA T^CATGA
966 TCATGA
BspLU11I 1085 ACATGT A^CATGT
2456 ACATGT
BsrBI 96 CCGCTC CCGCTC(-3/-3)
2231 GAGCGG
EagI 82 CGGCCG C^GGCCG
4060 CGGCCG
EclXI 82 CGGCCG C^GGCCG
4060 CGGCCG
Eco47III 2521 AGCGCT AGC^GCT
4351 AGCGCT
Eco52I 82 CGGCCG C^GGCCG
4060 CGGCCG
EcoNI 873 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3190 CCTGCTGCAGG
FbaI 969 TGATCA T^GATCA
3559 TGATCA
MslI 439 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1114 CATGGACGTG
PflMI 315 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3894 CCACGGGTTGG
PshAI 541 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3596 GACAGCAGTC
RcaI 207 TCATGA T^CATGA
966 TCATGA
SacI 853 GAGCTC GAGCT^C
1223 GAGCTC
SbfI 2398 CCTGCAGG CCTGCA^GG
3671 CCTGCAGG
ScaI 480 AGTACT AGT^ACT
525 AGTACT
Sse8387I 2398 CCTGCAGG CCTGCA^GG
3671 CCTGCAGG
StuI 2022 AGGCCT AGG^CCT
2082 AGGCCT
Van91I 315 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3894 CCACGGGTTGG
XbaI 3225 TCTAGA T^CTAGA
3447 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaII AviII BalI BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII