##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AhdI AseI
AsnI AspEI AviII BcgI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BssSI BstBI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI HpaI MfeI
MluI MroI MunI NotI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 1242 GAATTC [GA]^AATT[CT]
AflIII 2024 ACGTGT A^C[GA][CT]GT
AgeI 2418 ACCGGT A^CCGGT
Alw44I 1343 GTGCAC G^TGCAC
AocI 4202 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2750 AGCGCT AGC^GCT
ApaLI 1343 GTGCAC G^TGCAC
ApoI 1242 GAATTC [GA]^AATT[CT]
AscI 2864 GGCGCGCC GG^CGCGCC
BglI 3898 GCCAGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 45 AGATCT A^GATCT
BlpI 437 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 437 GCTCAGC GC^T[ACGT]AGC
BsaBI 930 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3418 GAGACG CGTCTC(1/5)
Bsp1407I 887 TGTACA T^GTACA
BsrDI 1868 GCAATG GCAATG(2/0)
BsrGI 887 TGTACA T^GTACA
Bst1107I 981 GTATAC GTA^TAC
BstAPI 2043 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 981 GTATAC GTA^TAC
Bsu36I 4202 CCTCAGG CC^T[ACGT]AGG
CelII 437 GCTCAGC GC^T[ACGT]AGC
DraI 4954 TTTAAA TTT^AAA
DraIII 281 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2750 AGCGCT AGC^GCT
Eco81I 4202 CCTCAGG CC^T[ACGT]AGG
EcoRI 1242 GAATTC G^AATTC
HindIII 4828 AAGCTT A^AGCTT
MamI 930 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1760 CCATGG C^CATGG
NdeI 2068 CATATG CA^TATG
NheI 4332 GCTAGC G^CTAGC
NspI 2045 ACATGC [GA]CATG^[CT]
PinAI 2418 ACCGGT A^CCGGT
PpuMI 4783 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 3254 GAGCTC GAGCT^C
SspBI 887 TGTACA T^GTACA
XbaI 4184 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 476 GGTACC G^GTACC
820 GGTACC
AccI 981 GTATAC GT^[AC][GT]AC
3714 GTCTAC
AflII 178 CTTAAG C^TTAAG
4344 CTTAAG
Asp700I 1893 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4980 GAATGTTTTC
Asp718I 476 GGTACC G^GTACC
820 GGTACC
AspI 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGTGGTC
AvrII 18 CCTAGG C^CTAGG
3927 CCTAGG
BbeI 3158 GGCGCC GGCGC^C
3425 GGCGCC
BbrPI 2023 CACGTG CAC^GTG
4847 CACGTG
BbvCI 3838 CCTCAGC CCTCAGC(-5/-2)
2541 GCTGAGG
BciVI 3891 GTATCC GTATCC(6/5)
4028 GTATCC
BclI 1398 TGATCA T^GATCA
4735 TGATCA
BfrI 178 CTTAAG C^TTAAG
4344 CTTAAG
BlnI 18 CCTAGG C^CTAGG
3927 CCTAGG
BsaWI 2282 TCCGGT [AT]^CCGG[AT]
2418 ACCGGT
BseRI 1202 GAGGAG GAGGAG(10/8)
1320 GAGGAG
BsmI 1893 GAATGC GAATGC(1/-1)
1897 GCATTC
EcoT22I 950 ATGCAT ATGCA^T
1895 ATGCAT
FbaI 1398 TGATCA T^GATCA
4735 TGATCA
HincII 1063 GTTGAC GT[CT]^[GA]AC
2341 GTCAAC
HindII 1063 GTTGAC GT[CT]^[GA]AC
2341 GTCAAC
KasI 3158 GGCGCC G^GCGCC
3425 GGCGCC
KpnI 476 GGTACC GGTAC^C
820 GGTACC
KspI 1903 CCGCGG CCGC^GG
3081 CCGCGG
NaeI 514 GCCGGC GCC^GGC
3218 GCCGGC
NarI 3158 GGCGCC GG^CGCC
3425 GGCGCC
NgoMIV 514 GCCGGC G^CCGGC
3218 GCCGGC
NsiI 950 ATGCAT ATGCA^T
1895 ATGCAT
PaeR7I 2198 CTCGAG C^TCGAG
3355 CTCGAG
PflFI 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGTGGTC
PmaCI 2023 CACGTG CAC^GTG
4847 CACGTG
PmlI 2023 CACGTG CAC^GTG
4847 CACGTG
PshAI 2362 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3633 GACCACCGTC
SacII 1903 CCGCGG CCGC^GG
3081 CCGCGG
SapI 2536 GAAGAGC GCTCTTC(1/4)
3952 GAAGAGC
SfoI 3158 GGCGCC GGC^GCC
3425 GGCGCC
StuI 1265 AGGCCT AGG^CCT
2466 AGGCCT
TaqI 2199 TCGA T^CGA
3356 TCGA
Tsp509I 1243 AATT ^AATT
4425 AATT
Tth111I 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGTGGTC
TthHB8I 2199 TCGA T^CGA
3356 TCGA
XhoI 2198 CTCGAG C^TCGAG
3355 CTCGAG
XmnI 1893 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4980 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I TspRI
Van91I XcmI XhoII XmaCI XmaI