################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AccIII AclI AhdI AseI AsnI AspEI AviII BcgI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BssSI BstBI ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI HpaI MfeI MluI MroI MunI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 1242 GAATTC [GA]^AATT[CT] AflIII 2024 ACGTGT A^C[GA][CT]GT AgeI 2418 ACCGGT A^CCGGT Alw44I 1343 GTGCAC G^TGCAC AocI 4202 CCTCAGG CC^T[ACGT]AGG Aor51HI 2750 AGCGCT AGC^GCT ApaLI 1343 GTGCAC G^TGCAC ApoI 1242 GAATTC [GA]^AATT[CT] AscI 2864 GGCGCGCC GG^CGCGCC BglI 3898 GCCAGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 45 AGATCT A^GATCT BlpI 437 GCTCAGC GC^T[ACGT]AGC Bpu1102I 437 GCTCAGC GC^T[ACGT]AGC BsaBI 930 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3418 GAGACG CGTCTC(1/5) Bsp1407I 887 TGTACA T^GTACA BsrDI 1868 GCAATG GCAATG(2/0) BsrGI 887 TGTACA T^GTACA Bst1107I 981 GTATAC GTA^TAC BstAPI 2043 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 981 GTATAC GTA^TAC Bsu36I 4202 CCTCAGG CC^T[ACGT]AGG CelII 437 GCTCAGC GC^T[ACGT]AGC DraI 4954 TTTAAA TTT^AAA DraIII 281 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2750 AGCGCT AGC^GCT Eco81I 4202 CCTCAGG CC^T[ACGT]AGG EcoRI 1242 GAATTC G^AATTC HindIII 4828 AAGCTT A^AGCTT MamI 930 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1760 CCATGG C^CATGG NdeI 2068 CATATG CA^TATG NheI 4332 GCTAGC G^CTAGC NspI 2045 ACATGC [GA]CATG^[CT] PinAI 2418 ACCGGT A^CCGGT PpuMI 4783 GGGTCCC [GA]G^G[AT]CC[CT] SacI 3254 GAGCTC GAGCT^C SspBI 887 TGTACA T^GTACA XbaI 4184 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 476 GGTACC G^GTACC 820 GGTACC AccI 981 GTATAC GT^[AC][GT]AC 3714 GTCTAC AflII 178 CTTAAG C^TTAAG 4344 CTTAAG Asp700I 1893 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4980 GAATGTTTTC Asp718I 476 GGTACC G^GTACC 820 GGTACC AspI 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACGTGGTC AvrII 18 CCTAGG C^CTAGG 3927 CCTAGG BbeI 3158 GGCGCC GGCGC^C 3425 GGCGCC BbrPI 2023 CACGTG CAC^GTG 4847 CACGTG BbvCI 3838 CCTCAGC CCTCAGC(-5/-2) 2541 GCTGAGG BciVI 3891 GTATCC GTATCC(6/5) 4028 GTATCC BclI 1398 TGATCA T^GATCA 4735 TGATCA BfrI 178 CTTAAG C^TTAAG 4344 CTTAAG BlnI 18 CCTAGG C^CTAGG 3927 CCTAGG BsaWI 2282 TCCGGT [AT]^CCGG[AT] 2418 ACCGGT BseRI 1202 GAGGAG GAGGAG(10/8) 1320 GAGGAG BsmI 1893 GAATGC GAATGC(1/-1) 1897 GCATTC EcoT22I 950 ATGCAT ATGCA^T 1895 ATGCAT FbaI 1398 TGATCA T^GATCA 4735 TGATCA HincII 1063 GTTGAC GT[CT]^[GA]AC 2341 GTCAAC HindII 1063 GTTGAC GT[CT]^[GA]AC 2341 GTCAAC KasI 3158 GGCGCC G^GCGCC 3425 GGCGCC KpnI 476 GGTACC GGTAC^C 820 GGTACC KspI 1903 CCGCGG CCGC^GG 3081 CCGCGG NaeI 514 GCCGGC GCC^GGC 3218 GCCGGC NarI 3158 GGCGCC GG^CGCC 3425 GGCGCC NgoMIV 514 GCCGGC G^CCGGC 3218 GCCGGC NsiI 950 ATGCAT ATGCA^T 1895 ATGCAT PaeR7I 2198 CTCGAG C^TCGAG 3355 CTCGAG PflFI 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACGTGGTC PmaCI 2023 CACGTG CAC^GTG 4847 CACGTG PmlI 2023 CACGTG CAC^GTG 4847 CACGTG PshAI 2362 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3633 GACCACCGTC SacII 1903 CCGCGG CCGC^GG 3081 CCGCGG SapI 2536 GAAGAGC GCTCTTC(1/4) 3952 GAAGAGC SfoI 3158 GGCGCC GGC^GCC 3425 GGCGCC StuI 1265 AGGCCT AGG^CCT 2466 AGGCCT TaqI 2199 TCGA T^CGA 3356 TCGA Tsp509I 1243 AATT ^AATT 4425 AATT Tth111I 573 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACGTGGTC TthHB8I 2199 TCGA T^CGA 3356 TCGA XhoI 2198 CTCGAG C^TCGAG 3355 CTCGAG XmnI 1893 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4980 GAATGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XcmI XhoII XmaCI XmaI