##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI AspI
AviII AvrII BbeI BbrPI BclI
BglI BlnI BsaAI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrFI BssHII BstBI Cfr10I ClaI
CpoI DrdI Eco47III EcoT22I FbaI
FseI FspI HaeII KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2611 GGTACC G^GTACC
AclI 209 AACGTT AA^CGTT
AflII 3709 CTTAAG C^TTAAG
AhdI 2085 GACGACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 840 GTGCAC G^TGCAC
ApaLI 840 GTGCAC G^TGCAC
Asp700I 853 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2611 GGTACC G^GTACC
AspEI 2085 GACGACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 2428 CCTCAGC CCTCAGC(-5/-2)
BcgI 485 CGATGAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1298 GTATCC GTATCC(6/5)
BfrI 3709 CTTAAG C^TTAAG
BglII 1252 AGATCT A^GATCT
BlpI 2592 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2592 GCTGAGC GC^T[ACGT]AGC
BsgI 303 CTGCAC GTGCAG(16/14)
BsiEI 2542 CGGCCG CG[GA][CT]^CG
BspHI 3536 TCATGA T^CATGA
BsrBI 1979 GAGCGG CCGCTC(-3/-3)
BssSI 162 CTCGTG CACGAG(-5/-1)
Bst1107I 2099 GTATAC GTA^TAC
BstAPI 76 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3765 GGTGACC G^GT[ACGT]ACC
BstPI 3765 GGTGACC G^GT[ACGT]ACC
BstXI 2905 CCACTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2099 GTATAC GTA^TAC
CelII 2592 GCTGAGC GC^T[ACGT]AGC
CfoI 3040 GCGC GCG^C
DraI 37 TTTAAA TTT^AAA
DraIII 3958 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2542 CGGCCG C^GGCCG
Eam1105I 2085 GACGACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2542 CGGCCG C^GGCCG
Eco52I 2542 CGGCCG C^GGCCG
EcoO65I 3765 GGTGACC G^GT[ACGT]ACC
EcoRI 1735 GAATTC G^AATTC
HhaI 3040 GCGC GCG^C
HinP1I 3040 GCGC G^CGC
HincII 2795 GTTAAC GT[CT]^[GA]AC
HindII 2795 GTTAAC GT[CT]^[GA]AC
HpaI 2795 GTTAAC GTT^AAC
KpnI 2611 GGTACC GGTAC^C
PaeR7I 3795 CTCGAG C^TCGAG
PflMI 660 CCAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 209 AACGTT AA^CGTT
RcaI 3536 TCATGA T^CATGA
ScaI 196 AGTACT AGT^ACT
SexAI 906 ACCTGGT A^CC[AT]GGT
SpeI 3052 ACTAGT A^CTAGT
Van91I 660 CCAACGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3795 CTCGAG C^TCGAG
XmnI 853 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 2496 CGCG CG^CG
3041 CGCG
AcyI 167 GGCGTC G[GA]^CG[CT]C
2135 GACGCC
AocI 1132 CCTCAGG CC^T[ACGT]AGG
1326 CCTCAGG
AvaI 613 CTCGGG C^[CT]CG[GA]G
3795 CTCGAG
BalI 949 TGGCCA TGG^CCA
3684 TGGCCA
BanI 2611 GGTACC G^G[CT][GA]CC
2689 GGTGCC
BcnI 288 CCCGG CC^[GC]GG
3259 CCCGG
BsaHI 167 GGCGTC G[GA]^CG[CT]C
2135 GACGCC
BsaWI 979 ACCGGA [AT]^CCGG[AT]
2310 TCCGGT
BsoBI 613 CTCGGG C^[CT]CG[GA]G
3795 CTCGAG
Bsp1407I 395 TGTACA T^GTACA
899 TGTACA
BsrDI 1114 GCAATG GCAATG(2/0)
3580 GCAATG
BsrGI 395 TGTACA T^GTACA
899 TGTACA
BstUI 2496 CGCG CG^CG
3041 CGCG
Bsu36I 1132 CCTCAGG CC^T[ACGT]AGG
1326 CCTCAGG
Eco81I 1132 CCTCAGG CC^T[ACGT]AGG
1326 CCTCAGG
EcoNI 1259 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3161 CCTGGGGGAGG
EcoRV 1127 GATATC GAT^ATC
2329 GATATC
Hin1I 167 GGCGTC G[GA]^CG[CT]C
2135 GACGCC
HindIII 135 AAGCTT A^AGCTT
1016 AAGCTT
MluNI 949 TGGCCA TGG^CCA
3684 TGGCCA
MscI 949 TGGCCA TGG^CCA
3684 TGGCCA
MvnI 2496 CGCG CG^CG
3041 CGCG
NciI 288 CCCGG CC^[GC]GG
3259 CCCGG
NcoI 708 CCATGG C^CATGG
1033 CCATGG
PshAI 222 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3231 GACTCTTGTC
SacI 1272 GAGCTC GAGCT^C
4054 GAGCTC
SphI 1891 GCATGC GCATG^C
2239 GCATGC
SspBI 395 TGTACA T^GTACA
899 TGTACA
StuI 388 AGGCCT AGG^CCT
2581 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BamHI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII