##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI AviII
BbeI BciVI BclI BlpI Bpu1102I
BsaWI BseAI BsiWI BspEI BspHI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII CpoI Eco47III EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MluI MroI NarI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PvuI RcaI RsrII SacI SacII
SalI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 1443 GTAGAC GT^[AC][GT]AC
AccII 2362 CGCG CG^CG
AclI 3389 AACGTT AA^CGTT
AhdI 3023 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3439 CCTTAGG CC^T[ACGT]AGG
ApaI 834 GGGCCC GGGCC^C
AseI 2135 ATTAAT AT^TAAT
AsnI 2135 ATTAAT AT^TAAT
AspEI 3023 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 4502 GAGCAC G[AT]GC[AT]^C
AvrII 1906 CCTAGG C^CTAGG
BamHI 414 GGATCC G^GATCC
BbrPI 902 CACGTG CAC^GTG
BbvCI 4142 GCTGAGG CCTCAGC(-5/-2)
BglI 1130 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1906 CCTAGG C^CTAGG
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsiHKAI 4502 GAGCAC G[AT]GC[AT]^C
BsmBI 2380 CGTCTC CGTCTC(1/5)
Bsp1407I 2141 TGTACA T^GTACA
BspDI 2274 ATCGAT AT^CGAT
BspLU11I 3543 ACATGT A^CATGT
BsrBI 2709 GAGCGG CCGCTC(-3/-3)
BsrDI 3617 GCAATG GCAATG(2/0)
BsrGI 2141 TGTACA T^GTACA
BstEII 3627 GGTGACC G^GT[ACGT]ACC
BstPI 3627 GGTGACC G^GT[ACGT]ACC
BstUI 2362 CGCG CG^CG
Bsu36I 3439 CCTTAGG CC^T[ACGT]AGG
ClaI 2274 ATCGAT AT^CGAT
DraI 1390 TTTAAA TTT^AAA
DraIII 401 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 971 GACCTGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 17 CGGCCG C^GGCCG
Eam1105I 3023 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
Eco81I 3439 CCTTAGG CC^T[ACGT]AGG
EcoNI 859 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3627 GGTGACC G^GT[ACGT]ACC
EcoRI 2187 GAATTC G^AATTC
HincII 818 GTTGAC GT[CT]^[GA]AC
HindII 818 GTTGAC GT[CT]^[GA]AC
MvnI 2362 CGCG CG^CG
NaeI 15 GCCGGC GCC^GGC
NdeI 2399 CATATG CA^TATG
NgoMIV 15 GCCGGC G^CCGGC
PaeR7I 4371 CTCGAG C^TCGAG
PmaCI 902 CACGTG CAC^GTG
PmlI 902 CACGTG CAC^GTG
PpuMI 1170 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2135 ATTAAT AT^TAAT
Psp1406I 3389 AACGTT AA^CGTT
PstI 257 CTGCAG CTGCA^G
PvuII 4328 CAGCTG CAG^CTG
SapI 1040 GAAGAGC GCTCTTC(1/4)
SbfI 256 CCTGCAGG CCTGCA^GG
SfiI 1129 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 256 CCTGCAGG CCTGCA^GG
SspBI 2141 TGTACA T^GTACA
XhoI 4371 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 607 GACGTC GACGT^C
4356 GACGTC
AcyI 607 GACGTC G[GA]^CG[CT]C
4356 GACGTC
BcgI 435 GCAGCCTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4363 GCACGGGTCTCG
BglII 2291 AGATCT A^GATCT
2459 AGATCT
BsaBI 1690 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4253 GATCATAATC
BsaHI 607 GACGTC G[GA]^CG[CT]C
4356 GACGTC
BstXI 564 CCACCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1175 CCAGAGACCTGG
EcoRV 1957 GATATC GAT^ATC
2271 GATATC
HaeII 4120 GGCGCT [GA]GCGC^[CT]
4333 GGCGCT
HgaI 963 GCGTC GACGC(5/10)
2379 GCGTC
Hin1I 607 GACGTC G[GA]^CG[CT]C
4356 GACGTC
MamI 1690 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4253 GATCATAATC
MfeI 2411 CAATTG C^AATTG
4961 CAATTG
MspA1I 2317 CAGCGG C[AC]G^C[GT]G
4328 CAGCTG
MunI 2411 CAATTG C^AATTG
4961 CAATTG
NcoI 54 CCATGG C^CATGG
4497 CCATGG
ScaI 912 AGTACT AGT^ACT
4941 AGTACT
XcmI 418 CCATTACCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3491 CCACACCCAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI Asp700I
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI
XmnI