##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaI ApaLI AscI Asp700I Asp718I
AspI AviII BanI BbeI BcgI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI FspI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 307 GACGTC GACGT^C
AccII 817 CGCG CG^CG
AcyI 307 GACGTC G[GA]^CG[CT]C
AflII 2856 CTTAAG C^TTAAG
AhdI 608 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 166 AGCGCT AGC^GCT
AseI 3790 ATTAAT AT^TAAT
AsnI 3790 ATTAAT AT^TAAT
AspEI 608 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1752 CCCGGG C^[CT]CG[GA]G
AvrII 3811 CCTAGG C^CTAGG
BamHI 2599 GGATCC G^GATCC
BbrPI 291 CACGTG CAC^GTG
BbvCI 3848 GCTGAGG CCTCAGC(-5/-2)
BciVI 661 GGATAC GTATCC(6/5)
BclI 1446 TGATCA T^GATCA
BfrI 2856 CTTAAG C^TTAAG
BglI 1614 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3191 AGATCT A^GATCT
BlnI 3811 CCTAGG C^CTAGG
BsaAI 291 CACGTG [CT]AC^GT[GA]
BsaHI 307 GACGTC G[GA]^CG[CT]C
BsmBI 305 GAGACG CGTCTC(1/5)
BsoBI 1752 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2269 TGTACA T^GTACA
BspHI 7 TCATGA T^CATGA
BspLU11I 2491 ACATGT A^CATGT
BsrBI 1747 CCGCTC CCGCTC(-3/-3)
BsrDI 3117 CATTGC GCAATG(2/0)
BsrGI 2269 TGTACA T^GTACA
BssSI 1767 CTCGTG CACGAG(-5/-1)
BstUI 817 CGCG CG^CG
Eam1105I 608 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 166 AGCGCT AGC^GCT
EcoT22I 2315 ATGCAT ATGCA^T
FauI 2960 GCGGG CCCGC(4/6)
FbaI 1446 TGATCA T^GATCA
HaeII 166 AGCGCT [GA]GCGC^[CT]
HapII 1753 CCGG C^CGG
Hin1I 307 GACGTC G[GA]^CG[CT]C
HindIII 3299 AAGCTT A^AGCTT
HpaI 2648 GTTAAC GTT^AAC
HpaII 1753 CCGG C^CGG
MspI 1753 CCGG C^CGG
MvnI 817 CGCG CG^CG
NcoI 3511 CCATGG C^CATGG
NdeI 4182 CATATG CA^TATG
NheI 2754 GCTAGC G^CTAGC
NsiI 2315 ATGCAT ATGCA^T
PmaCI 291 CACGTG CAC^GTG
PmlI 291 CACGTG CAC^GTG
PshAI 1325 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3790 ATTAAT AT^TAAT
RcaI 7 TCATGA T^CATGA
SmaI 1752 CCCGGG CCC^GGG
SpeI 53 ACTAGT A^CTAGT
SspBI 2269 TGTACA T^GTACA
XmaCI 1752 CCCGGG C^CCGGG
XmaI 1752 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1417 AAATTC [GA]^AATT[CT]
2745 AAATTT
AflIII 290 ACACGT A^C[GA][CT]GT
2491 ACATGT
AocI 1283 CCTCAGG CC^T[ACGT]AGG
3351 CCTTAGG
ApoI 1417 AAATTC [GA]^AATT[CT]
2745 AAATTT
BalI 1575 TGGCCA TGG^CCA
2004 TGGCCA
BanII 571 GAGCTC G[GA]GC[CT]^C
3634 GAGCTC
BbsI 412 GTCTTC GAAGAC(2/6)
3486 GTCTTC
BcnI 1752 CCCGG CC^[GC]GG
1753 CCGGG
BlpI 1690 GCTCAGC GC^T[ACGT]AGC
2178 GCTAAGC
Bpu1102I 1690 GCTCAGC GC^T[ACGT]AGC
2178 GCTAAGC
BpuAI 412 GTCTTC GAAGAC(2/6)
3486 GTCTTC
BsmI 3904 GAATGC GAATGC(1/-1)
4030 GAATGC
Bsu36I 1283 CCTCAGG CC^T[ACGT]AGG
3351 CCTTAGG
CelII 1690 GCTCAGC GC^T[ACGT]AGC
2178 GCTAAGC
DraI 953 TTTAAA TTT^AAA
1297 TTTAAA
DraIII 2046 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2580 CACTGTGTG
Eco81I 1283 CCTCAGG CC^T[ACGT]AGG
3351 CCTTAGG
EcoNI 154 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2888 CCTCCATGAGG
HgaI 410 GCGTC GACGC(5/10)
614 GCGTC
MluNI 1575 TGGCCA TGG^CCA
2004 TGGCCA
MscI 1575 TGGCCA TGG^CCA
2004 TGGCCA
NciI 1752 CCCGG CC^[GC]GG
1753 CCGGG
PpuMI 2385 GGGACCT [GA]G^G[AT]CC[CT]
3029 GGGTCCT
SacI 571 GAGCTC GAGCT^C
3634 GAGCTC
ScaI 56 AGTACT AGT^ACT
2852 AGTACT
SspI 746 AATATT AAT^ATT
1030 AATATT
TaqI 2349 TCGA T^CGA
3806 TCGA
TthHB8I 2349 TCGA T^CGA
3806 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII