##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AflII AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
BbrPI BclI BfrI BsaAI BseAI
BsiWI BspDI BspEI BstBI ClaI
CpoI DraI Eam1105I EcoRV FbaI
FseI HpaI MfeI MluI MroI
MunI NdeI NruI NspV PacI
PmaCI PmeI PmlI PshBI PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AclI 1102 AACGTT AA^CGTT
AcsI 4173 GAATTC [GA]^AATT[CT]
AgeI 1723 ACCGGT A^CCGGT
AocI 2727 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2540 AGCGCT AGC^GCT
ApoI 4173 GAATTC [GA]^AATT[CT]
Asp700I 2298 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2461 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2651 TGCGCA TGC^GCA
BamHI 1333 GGATCC G^GATCC
BlpI 88 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 88 GCTGAGC GC^T[ACGT]AGC
BsiEI 755 CGGCCG CG[GA][CT]^CG
BsmBI 736 GAGACG CGTCTC(1/5)
BssSI 3735 CTCGTG CACGAG(-5/-1)
Bst1107I 3555 GTATAC GTA^TAC
BstAPI 3378 GCACTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3555 GTATAC GTA^TAC
Bsu36I 2727 CCTCAGG CC^T[ACGT]AGG
CelII 88 GCTGAGC GC^T[ACGT]AGC
DraIII 2626 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 755 CGGCCG C^GGCCG
EclXI 755 CGGCCG C^GGCCG
Eco47III 2540 AGCGCT AGC^GCT
Eco52I 755 CGGCCG C^GGCCG
Eco81I 2727 CCTCAGG CC^T[ACGT]AGG
EcoRI 4173 GAATTC G^AATTC
EcoT22I 3073 ATGCAT ATGCA^T
FspI 2651 TGCGCA TGC^GCA
HincII 841 GTCAAC GT[CT]^[GA]AC
HindII 841 GTCAAC GT[CT]^[GA]AC
HindIII 4556 AAGCTT A^AGCTT
KspI 1986 CCGCGG CCGC^GG
MseI 4540 TTAA T^TAA
NcoI 263 CCATGG C^CATGG
NheI 4051 GCTAGC G^CTAGC
NotI 754 GCGGCCGC GC^GGCCGC
NsiI 3073 ATGCAT ATGCA^T
PaeR7I 3196 CTCGAG C^TCGAG
PflFI 2461 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1723 ACCGGT A^CCGGT
PshAI 5029 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1102 AACGTT AA^CGTT
SacI 4008 GAGCTC GAGCT^C
SacII 1986 CCGCGG CCGC^GG
SapI 3578 GCTCTTC GCTCTTC(1/4)
ScaI 1085 AGTACT AGT^ACT
SexAI 1502 ACCTGGT A^CC[AT]GGT
SmaI 4549 CCCGGG CCC^GGG
SmlI 3196 CTCGAG C^T[CT][GA]AG
Tru9I 4540 TTAA T^TAA
Tth111I 2461 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 665 CCATGTACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3196 CTCGAG C^TCGAG
XmaCI 4549 CCCGGG C^CCGGG
XmaI 4549 CCCGGG C^CCGGG
XmnI 2298 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1790 ACATGT A^C[GA][CT]GT
3400 ACATGT
ApaI 1691 GGGCCC GGGCC^C
2071 GGGCCC
AspHI 1164 GAGCAC G[AT]GC[AT]^C
4008 GAGCTC
AvrII 3459 CCTAGG C^CTAGG
4003 CCTAGG
BbvCI 4310 CCTCAGC CCTCAGC(-5/-2)
4316 CCTCAGC
BcgI 380 GCACCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4845 GCAGGTGGCTCG
BciVI 1727 GTATCC GTATCC(6/5)
3811 GTATCC
BglII 689 AGATCT A^GATCT
3566 AGATCT
BlnI 3459 CCTAGG C^CTAGG
4003 CCTAGG
BsaBI 1911 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2649 GATGCGCATC
BsaI 1720 GAGACC GGTCTC(1/5)
2273 GGTCTC
BsaWI 1723 ACCGGT [AT]^CCGG[AT]
2270 TCCGGT
BsiHKAI 1164 GAGCAC G[AT]GC[AT]^C
4008 GAGCTC
BspLU11I 1790 ACATGT A^CATGT
3400 ACATGT
BsrBI 1341 CCGCTC CCGCTC(-3/-3)
2857 GAGCGG
BssHII 58 GCGCGC G^CGCGC
60 GCGCGC
BstEII 3418 GGTCACC G^GT[ACGT]ACC
3948 GGTCACC
BstPI 3418 GGTCACC G^GT[ACGT]ACC
3948 GGTCACC
DrdI 3 GACAGCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
934 GACATCCCGGTC
EcoNI 306 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1608 CCTACTGCAGG
EcoO65I 3418 GGTCACC G^GT[ACGT]ACC
3948 GGTCACC
MamI 1911 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2649 GATGCGCATC
NaeI 1235 GCCGGC GCC^GGC
1957 GCCGGC
NgoMIV 1235 GCCGGC G^CCGGC
1957 GCCGGC
StuI 1487 AGGCCT AGG^CCT
2492 AGGCCT
Tsp509I 4174 AATT ^AATT
4797 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI Asp718I
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SphI SspBI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XbaI XhoII