##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AgeI AhdI AlwNI Aor51HI
AscI AspEI AspI AvrII BbsI
BcgI BciVI BclI BlnI BpuAI
BsiEI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstXI ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI HpaI
MluI NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PvuI
RsrII SalI SbfI SexAI SfiI
SgrAI SphI Sse8387I SspBI StuI
Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 3646 GTATAC GT^[AC][GT]AC
AccII 18 CGCG CG^CG
AccIII 1092 TCCGGA T^CCGGA
AclI 1136 AACGTT AA^CGTT
AcyI 2972 GGCGCC G[GA]^CG[CT]C
Alw44I 4172 GTGCAC G^TGCAC
AocI 2738 CCTCAGG CC^T[ACGT]AGG
ApaI 485 GGGCCC GGGCC^C
ApaLI 4172 GTGCAC G^TGCAC
AseI 5311 ATTAAT AT^TAAT
AsnI 5311 ATTAAT AT^TAAT
AvaI 199 CCCGGG C^[CT]CG[GA]G
AviII 3570 TGCGCA TGC^GCA
BamHI 3082 GGATCC G^GATCC
BbeI 2972 GGCGCC GGCGC^C
BbrPI 4645 CACGTG CAC^GTG
BbvCI 2360 GCTGAGG CCTCAGC(-5/-2)
BglI 61 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3876 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3876 GCTTAGC GC^T[ACGT]AGC
BsaHI 2972 GGCGCC G[GA]^CG[CT]C
BsaWI 1092 TCCGGA [AT]^CCGG[AT]
BseAI 1092 TCCGGA T^CCGGA
BsgI 1026 GTGCAG GTGCAG(16/14)
BsmBI 861 GAGACG CGTCTC(1/5)
BsoBI 199 CCCGGG C^[CT]CG[GA]G
BspEI 1092 TCCGGA T^CCGGA
BsrBI 2897 GAGCGG CCGCTC(-3/-3)
Bst1107I 3646 GTATAC GTA^TAC
BstBI 1140 TTCGAA TT^CGAA
BstUI 18 CGCG CG^CG
BstZ17I 3646 GTATAC GTA^TAC
Bsu36I 2738 CCTCAGG CC^T[ACGT]AGG
CelII 3876 GCTTAGC GC^T[ACGT]AGC
DrdI 350 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2738 CCTCAGG CC^T[ACGT]AGG
EcoNI 3440 CCTACAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3570 TGCGCA TGC^GCA
HaeII 2972 GGCGCC [GA]GCGC^[CT]
Hin1I 2972 GGCGCC G[GA]^CG[CT]C
KasI 2972 GGCGCC G^GCGCC
KspI 17 CCGCGG CCGC^GG
MroI 1092 TCCGGA T^CCGGA
MvnI 18 CGCG CG^CG
NarI 2972 GGCGCC GG^CGCC
NdeI 4342 CATATG CA^TATG
NspV 1140 TTCGAA TT^CGAA
PmaCI 4645 CACGTG CAC^GTG
PmeI 3295 GTTTAAAC GTTT^AAAC
PmlI 4645 CACGTG CAC^GTG
PshAI 4679 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5311 ATTAAT AT^TAAT
Psp1406I 1136 AACGTT AA^CGTT
PstI 342 CTGCAG CTGCA^G
SacII 17 CCGCGG CCGC^GG
SfoI 2972 GGCGCC GGC^GCC
SfuI 1140 TTCGAA TT^CGAA
SmaI 199 CCCGGG CCC^GGG
SnaBI 3643 TACGTA TAC^GTA
SpeI 2800 ACTAGT A^CTAGT
SwaI 4880 ATTTAAAT ATTT^AAAT
XmaCI 199 CCCGGG C^CCGGG
XmaI 199 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 3469 GGTACC G^GTACC
4163 GGTACC
AflII 1841 CTTAAG C^TTAAG
5014 CTTAAG
Asp718I 3469 GGTACC G^GTACC
4163 GGTACC
BalI 297 TGGCCA TGG^CCA
3597 TGGCCA
BfrI 1841 CTTAAG C^TTAAG
5014 CTTAAG
BglII 2821 AGATCT A^GATCT
4292 AGATCT
BsaBI 1704 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3061 GATTGTTATC
BsmFI 3866 GGGAC GGGAC(10/14)
3578 GTCCC
BspHI 2326 TCATGA T^CATGA
4081 TCATGA
CfoI 2973 GCGC GCG^C
3571 GCGC
EcoT22I 2414 ATGCAT ATGCA^T
4201 ATGCAT
HgaI 3055 GACGC GACGC(5/10)
76 GCGTC
HhaI 2973 GCGC GCG^C
3571 GCGC
HinP1I 2973 GCGC G^CGC
3571 GCGC
HincII 3275 GTTGAC GT[CT]^[GA]AC
4299 GTTGAC
HindII 3275 GTTGAC GT[CT]^[GA]AC
4299 GTTGAC
KpnI 3469 GGTACC GGTAC^C
4163 GGTACC
MamI 1704 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3061 GATTGTTATC
MfeI 247 CAATTG C^AATTG
1560 CAATTG
MluNI 297 TGGCCA TGG^CCA
3597 TGGCCA
MscI 297 TGGCCA TGG^CCA
3597 TGGCCA
MunI 247 CAATTG C^AATTG
1560 CAATTG
NaeI 25 GCCGGC GCC^GGC
3719 GCCGGC
NcoI 606 CCATGG C^CATGG
3465 CCATGG
NgoMIV 25 GCCGGC G^CCGGC
3719 GCCGGC
NsiI 2414 ATGCAT ATGCA^T
4201 ATGCAT
NspI 1228 ACATGC [GA]CATG^[CT]
4199 ACATGC
RcaI 2326 TCATGA T^CATGA
4081 TCATGA
SacI 3041 GAGCTC GAGCT^C
4021 GAGCTC
ScaI 1455 AGTACT AGT^ACT
5053 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI