################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AgeI AhdI AlwNI Aor51HI AscI AspEI AspI AvrII BbsI BcgI BciVI BclI BlnI BpuAI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstAPI BstEII BstPI BstXI ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FbaI FseI HpaI MluI NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PvuI RsrII SalI SbfI SexAI SfiI SgrAI SphI Sse8387I SspBI StuI Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 3646 GTATAC GT^[AC][GT]AC AccII 18 CGCG CG^CG AccIII 1092 TCCGGA T^CCGGA AclI 1136 AACGTT AA^CGTT AcyI 2972 GGCGCC G[GA]^CG[CT]C Alw44I 4172 GTGCAC G^TGCAC AocI 2738 CCTCAGG CC^T[ACGT]AGG ApaI 485 GGGCCC GGGCC^C ApaLI 4172 GTGCAC G^TGCAC AseI 5311 ATTAAT AT^TAAT AsnI 5311 ATTAAT AT^TAAT AvaI 199 CCCGGG C^[CT]CG[GA]G AviII 3570 TGCGCA TGC^GCA BamHI 3082 GGATCC G^GATCC BbeI 2972 GGCGCC GGCGC^C BbrPI 4645 CACGTG CAC^GTG BbvCI 2360 GCTGAGG CCTCAGC(-5/-2) BglI 61 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3876 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3876 GCTTAGC GC^T[ACGT]AGC BsaHI 2972 GGCGCC G[GA]^CG[CT]C BsaWI 1092 TCCGGA [AT]^CCGG[AT] BseAI 1092 TCCGGA T^CCGGA BsgI 1026 GTGCAG GTGCAG(16/14) BsmBI 861 GAGACG CGTCTC(1/5) BsoBI 199 CCCGGG C^[CT]CG[GA]G BspEI 1092 TCCGGA T^CCGGA BsrBI 2897 GAGCGG CCGCTC(-3/-3) Bst1107I 3646 GTATAC GTA^TAC BstBI 1140 TTCGAA TT^CGAA BstUI 18 CGCG CG^CG BstZ17I 3646 GTATAC GTA^TAC Bsu36I 2738 CCTCAGG CC^T[ACGT]AGG CelII 3876 GCTTAGC GC^T[ACGT]AGC DrdI 350 GACCTGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2738 CCTCAGG CC^T[ACGT]AGG EcoNI 3440 CCTACAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 3570 TGCGCA TGC^GCA HaeII 2972 GGCGCC [GA]GCGC^[CT] Hin1I 2972 GGCGCC G[GA]^CG[CT]C KasI 2972 GGCGCC G^GCGCC KspI 17 CCGCGG CCGC^GG MroI 1092 TCCGGA T^CCGGA MvnI 18 CGCG CG^CG NarI 2972 GGCGCC GG^CGCC NdeI 4342 CATATG CA^TATG NspV 1140 TTCGAA TT^CGAA PmaCI 4645 CACGTG CAC^GTG PmeI 3295 GTTTAAAC GTTT^AAAC PmlI 4645 CACGTG CAC^GTG PshAI 4679 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5311 ATTAAT AT^TAAT Psp1406I 1136 AACGTT AA^CGTT PstI 342 CTGCAG CTGCA^G SacII 17 CCGCGG CCGC^GG SfoI 2972 GGCGCC GGC^GCC SfuI 1140 TTCGAA TT^CGAA SmaI 199 CCCGGG CCC^GGG SnaBI 3643 TACGTA TAC^GTA SpeI 2800 ACTAGT A^CTAGT SwaI 4880 ATTTAAAT ATTT^AAAT XmaCI 199 CCCGGG C^CCGGG XmaI 199 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 3469 GGTACC G^GTACC 4163 GGTACC AflII 1841 CTTAAG C^TTAAG 5014 CTTAAG Asp718I 3469 GGTACC G^GTACC 4163 GGTACC BalI 297 TGGCCA TGG^CCA 3597 TGGCCA BfrI 1841 CTTAAG C^TTAAG 5014 CTTAAG BglII 2821 AGATCT A^GATCT 4292 AGATCT BsaBI 1704 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3061 GATTGTTATC BsmFI 3866 GGGAC GGGAC(10/14) 3578 GTCCC BspHI 2326 TCATGA T^CATGA 4081 TCATGA CfoI 2973 GCGC GCG^C 3571 GCGC EcoT22I 2414 ATGCAT ATGCA^T 4201 ATGCAT HgaI 3055 GACGC GACGC(5/10) 76 GCGTC HhaI 2973 GCGC GCG^C 3571 GCGC HinP1I 2973 GCGC G^CGC 3571 GCGC HincII 3275 GTTGAC GT[CT]^[GA]AC 4299 GTTGAC HindII 3275 GTTGAC GT[CT]^[GA]AC 4299 GTTGAC KpnI 3469 GGTACC GGTAC^C 4163 GGTACC MamI 1704 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3061 GATTGTTATC MfeI 247 CAATTG C^AATTG 1560 CAATTG MluNI 297 TGGCCA TGG^CCA 3597 TGGCCA MscI 297 TGGCCA TGG^CCA 3597 TGGCCA MunI 247 CAATTG C^AATTG 1560 CAATTG NaeI 25 GCCGGC GCC^GGC 3719 GCCGGC NcoI 606 CCATGG C^CATGG 3465 CCATGG NgoMIV 25 GCCGGC G^CCGGC 3719 GCCGGC NsiI 2414 ATGCAT ATGCA^T 4201 ATGCAT NspI 1228 ACATGC [GA]CATG^[CT] 4199 ACATGC RcaI 2326 TCATGA T^CATGA 4081 TCATGA SacI 3041 GAGCTC GAGCT^C 4021 GAGCTC ScaI 1455 AGTACT AGT^ACT 5053 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI