##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AgeI        AhdI        AlwNI       Aor51HI     
AscI        AspEI       AspI        AvrII       BbsI        
BcgI        BciVI       BclI        BlnI        BpuAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspLU11I    
BsrGI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstXI       ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       FbaI        FseI        HpaI        
MluI        NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PvuI        
RsrII       SalI        SbfI        SexAI       SfiI        
SgrAI       SphI        Sse8387I    SspBI       StuI        
Tth111I     Van91I      XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI         3646  GTATAC               GT^[AC][GT]AC
AccII          18  CGCG                 CG^CG
AccIII       1092  TCCGGA               T^CCGGA
AclI         1136  AACGTT               AA^CGTT
AcyI         2972  GGCGCC               G[GA]^CG[CT]C
Alw44I       4172  GTGCAC               G^TGCAC
AocI         2738  CCTCAGG              CC^T[ACGT]AGG
ApaI          485  GGGCCC               GGGCC^C
ApaLI        4172  GTGCAC               G^TGCAC
AseI         5311  ATTAAT               AT^TAAT
AsnI         5311  ATTAAT               AT^TAAT
AvaI          199  CCCGGG               C^[CT]CG[GA]G
AviII        3570  TGCGCA               TGC^GCA
BamHI        3082  GGATCC               G^GATCC
BbeI         2972  GGCGCC               GGCGC^C
BbrPI        4645  CACGTG               CAC^GTG
BbvCI        2360  GCTGAGG              CCTCAGC(-5/-2)
BglI           61  GCCGCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3876  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     3876  GCTTAGC              GC^T[ACGT]AGC
BsaHI        2972  GGCGCC               G[GA]^CG[CT]C
BsaWI        1092  TCCGGA               [AT]^CCGG[AT]
BseAI        1092  TCCGGA               T^CCGGA
BsgI         1026  GTGCAG               GTGCAG(16/14)
BsmBI         861  GAGACG               CGTCTC(1/5)
BsoBI         199  CCCGGG               C^[CT]CG[GA]G
BspEI        1092  TCCGGA               T^CCGGA
BsrBI        2897  GAGCGG               CCGCTC(-3/-3)
Bst1107I     3646  GTATAC               GTA^TAC
BstBI        1140  TTCGAA               TT^CGAA
BstUI          18  CGCG                 CG^CG
BstZ17I      3646  GTATAC               GTA^TAC
Bsu36I       2738  CCTCAGG              CC^T[ACGT]AGG
CelII        3876  GCTTAGC              GC^T[ACGT]AGC
DrdI          350  GACCTGGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2738  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3440  CCTACAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         3570  TGCGCA               TGC^GCA
HaeII        2972  GGCGCC               [GA]GCGC^[CT]
Hin1I        2972  GGCGCC               G[GA]^CG[CT]C
KasI         2972  GGCGCC               G^GCGCC
KspI           17  CCGCGG               CCGC^GG
MroI         1092  TCCGGA               T^CCGGA
MvnI           18  CGCG                 CG^CG
NarI         2972  GGCGCC               GG^CGCC
NdeI         4342  CATATG               CA^TATG
NspV         1140  TTCGAA               TT^CGAA
PmaCI        4645  CACGTG               CAC^GTG
PmeI         3295  GTTTAAAC             GTTT^AAAC
PmlI         4645  CACGTG               CAC^GTG
PshAI        4679  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5311  ATTAAT               AT^TAAT
Psp1406I     1136  AACGTT               AA^CGTT
PstI          342  CTGCAG               CTGCA^G
SacII          17  CCGCGG               CCGC^GG
SfoI         2972  GGCGCC               GGC^GCC
SfuI         1140  TTCGAA               TT^CGAA
SmaI          199  CCCGGG               CCC^GGG
SnaBI        3643  TACGTA               TAC^GTA
SpeI         2800  ACTAGT               A^CTAGT
SwaI         4880  ATTTAAAT             ATTT^AAAT
XmaCI         199  CCCGGG               C^CCGGG
XmaI          199  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I       3469  GGTACC               G^GTACC
             4163  GGTACC              
AflII        1841  CTTAAG               C^TTAAG
             5014  CTTAAG              
Asp718I      3469  GGTACC               G^GTACC
             4163  GGTACC              
BalI          297  TGGCCA               TGG^CCA
             3597  TGGCCA              
BfrI         1841  CTTAAG               C^TTAAG
             5014  CTTAAG              
BglII        2821  AGATCT               A^GATCT
             4292  AGATCT              
BsaBI        1704  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3061  GATTGTTATC          
BsmFI        3866  GGGAC                GGGAC(10/14)
             3578  GTCCC               
BspHI        2326  TCATGA               T^CATGA
             4081  TCATGA              
CfoI         2973  GCGC                 GCG^C
             3571  GCGC                
EcoT22I      2414  ATGCAT               ATGCA^T
             4201  ATGCAT              
HgaI         3055  GACGC                GACGC(5/10)
               76  GCGTC               
HhaI         2973  GCGC                 GCG^C
             3571  GCGC                
HinP1I       2973  GCGC                 G^CGC
             3571  GCGC                
HincII       3275  GTTGAC               GT[CT]^[GA]AC
             4299  GTTGAC              
HindII       3275  GTTGAC               GT[CT]^[GA]AC
             4299  GTTGAC              
KpnI         3469  GGTACC               GGTAC^C
             4163  GGTACC              
MamI         1704  GATATAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3061  GATTGTTATC          
MfeI          247  CAATTG               C^AATTG
             1560  CAATTG              
MluNI         297  TGGCCA               TGG^CCA
             3597  TGGCCA              
MscI          297  TGGCCA               TGG^CCA
             3597  TGGCCA              
MunI          247  CAATTG               C^AATTG
             1560  CAATTG              
NaeI           25  GCCGGC               GCC^GGC
             3719  GCCGGC              
NcoI          606  CCATGG               C^CATGG
             3465  CCATGG              
NgoMIV         25  GCCGGC               G^CCGGC
             3719  GCCGGC              
NsiI         2414  ATGCAT               ATGCA^T
             4201  ATGCAT              
NspI         1228  ACATGC               [GA]CATG^[CT]
             4199  ACATGC              
RcaI         2326  TCATGA               T^CATGA
             4081  TCATGA              
SacI         3041  GAGCTC               GAGCT^C
             4021  GAGCTC              
ScaI         1455  AGTACT               AGT^ACT
             5053  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaAI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmI        Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoRV       EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
XmnI