##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AgeI AscI Asp718I
AspI AviII AvrII BbeI BbvCI
BclI BlnI BsaBI BsiWI BspDI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoT22I FbaI FseI FspI
HpaI KasI KpnI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I RsrII SalI
ScaI SexAI SfiI SfoI SfuI
SnaBI SpeI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1673 GACGTC GACGT^C
AccIII 1951 TCCGGA T^CCGGA
AflII 843 CTTAAG C^TTAAG
AhdI 2541 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 130 AGCGCT AGC^GCT
ApaI 3680 GGGCCC GGGCC^C
Asp700I 1598 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2541 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1553 GGATCC G^GATCC
BcgI 62 CGATCGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4033 GGATAC GTATCC(6/5)
BfrI 843 CTTAAG C^TTAAG
BglI 2958 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2361 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2361 GCTCAGC GC^T[ACGT]AGC
BsaWI 1951 TCCGGA [AT]^CCGG[AT]
BseAI 1951 TCCGGA T^CCGGA
BsiEI 62 CGATCG CG[GA][CT]^CG
BspEI 1951 TCCGGA T^CCGGA
BspLU11I 4379 ACATGT A^CATGT
BstAPI 3998 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4023 CCATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2361 GCTCAGC GC^T[ACGT]AGC
DraI 4679 TTTAAA TTT^AAA
Eam1105I 2541 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 130 AGCGCT AGC^GCT
EcoRI 1632 GAATTC G^AATTC
EcoRV 1575 GATATC GAT^ATC
HindIII 232 AAGCTT A^AGCTT
KspI 114 CCGCGG CCGC^GG
MroI 1951 TCCGGA T^CCGGA
NcoI 1609 CCATGG C^CATGG
PvuI 62 CGATCG CGAT^CG
SacI 3499 GAGCTC GAGCT^C
SacII 114 CCGCGG CCGC^GG
SapI 1431 GAAGAGC GCTCTTC(1/4)
SgrAI 323 CACCGGCG C[GA]^CCGG[CT]G
SmaI 449 CCCGGG CCC^GGG
SphI 1047 GCATGC GCATG^C
StuI 2264 AGGCCT AGG^CCT
XcmI 3819 CCATCCTGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 449 CCCGGG C^CCGGG
XmaI 449 CCCGGG C^CCGGG
XmnI 1598 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3567 GTAGAC GT^[AC][GT]AC
4625 GTAGAC
AcyI 212 GACGCC G[GA]^CG[CT]C
1673 GACGTC
Alw44I 3701 GTGCAC G^TGCAC
4165 GTGCAC
AlwNI 2292 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
4080 CAGACACTG
ApaLI 3701 GTGCAC G^TGCAC
4165 GTGCAC
AseI 683 ATTAAT AT^TAAT
4457 ATTAAT
AsnI 683 ATTAAT AT^TAAT
4457 ATTAAT
BbrPI 1113 CACGTG CAC^GTG
3041 CACGTG
BsaAI 1113 CACGTG [CT]AC^GT[GA]
3041 CACGTG
BsaHI 212 GACGCC G[GA]^CG[CT]C
1673 GACGTC
BsaI 2335 GAGACC GGTCTC(1/5)
2776 GAGACC
BsmBI 1675 CGTCTC CGTCTC(1/5)
1671 GAGACG
Bsp1407I 3970 TGTACA T^GTACA
4568 TGTACA
BspHI 2039 TCATGA T^CATGA
4474 TCATGA
BsrBI 244 CCGCTC CCGCTC(-3/-3)
179 GAGCGG
BsrGI 3970 TGTACA T^GTACA
4568 TGTACA
BssHII 368 GCGCGC G^CGCGC
370 GCGCGC
BstEII 254 GGTAACC G^GT[ACGT]ACC
2030 GGTGACC
BstPI 254 GGTAACC G^GT[ACGT]ACC
2030 GGTGACC
EcoNI 1809 CCTCGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2476 CCTAAGGGAGG
EcoO65I 254 GGTAACC G^GT[ACGT]ACC
2030 GGTGACC
HaeII 130 AGCGCT [GA]GCGC^[CT]
411 AGCGCC
HgaI 212 GACGC GACGC(5/10)
2673 GCGTC
Hin1I 212 GACGCC G[GA]^CG[CT]C
1673 GACGTC
HincII 608 GTTGAC GT[CT]^[GA]AC
3471 GTTGAC
HindII 608 GTTGAC GT[CT]^[GA]AC
3471 GTTGAC
PflMI 1109 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3093 CCACCTTTTGG
PmaCI 1113 CACGTG CAC^GTG
3041 CACGTG
PmlI 1113 CACGTG CAC^GTG
3041 CACGTG
PpuMI 2439 AGGTCCC [GA]G^G[AT]CC[CT]
2491 AGGACCT
PshBI 683 ATTAAT AT^TAAT
4457 ATTAAT
RcaI 2039 TCATGA T^CATGA
4474 TCATGA
SbfI 1850 CCTGCAGG CCTGCA^GG
2509 CCTGCAGG
Sse8387I 1850 CCTGCAGG CCTGCA^GG
2509 CCTGCAGG
SspBI 3970 TGTACA T^GTACA
4568 TGTACA
Van91I 1109 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3093 CCACCTTTTGG
XbaI 3992 TCTAGA T^CTAGA
4324 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII