##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccII AccIII AgeI AhdI Aor51HI
AscI AspEI AvaI AviII AvrII
BalI BbeI BbrPI BglI BlnI
BsaBI BseAI BsiEI BsiWI BsoBI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FauI FseI FspI HaeII HgaI
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 634 GACGTC GACGT^C
AclI 2314 AACGTT AA^CGTT
AcyI 634 GACGTC G[GA]^CG[CT]C
AocI 328 CCTCAGG CC^T[ACGT]AGG
ApaI 1563 GGGCCC GGGCC^C
AseI 2971 ATTAAT AT^TAAT
AsnI 2971 ATTAAT AT^TAAT
AspI 1203 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 27 GGATCC G^GATCC
BbvCI 3339 GCTGAGG CCTCAGC(-5/-2)
BciVI 3044 GGATAC GTATCC(6/5)
BlpI 2432 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2432 GCTTAGC GC^T[ACGT]AGC
BsaAI 67 TACGTG [CT]AC^GT[GA]
BsaHI 634 GACGTC G[GA]^CG[CT]C
BsaWI 1580 TCCGGT [AT]^CCGG[AT]
BsmBI 401 GAGACG CGTCTC(1/5)
BsmI 1337 GCATTC GAATGC(1/-1)
BspDI 1613 ATCGAT AT^CGAT
BstAPI 586 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1495 GGTGACC G^GT[ACGT]ACC
BstPI 1495 GGTGACC G^GT[ACGT]ACC
BstXI 2246 CCACGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 328 CCTCAGG CC^T[ACGT]AGG
CelII 2432 GCTTAGC GC^T[ACGT]AGC
CfoI 3478 GCGC GCG^C
ClaI 1613 ATCGAT AT^CGAT
DraIII 3751 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 813 GACTCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1724 CGGCCA [CT]^GGCC[GA]
EarI 1406 CTCTTC CTCTTC(1/4)
Eco81I 328 CCTCAGG CC^T[ACGT]AGG
EcoNI 2844 CCTGGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1495 GGTGACC G^GT[ACGT]ACC
EcoRV 523 GATATC GAT^ATC
EcoT22I 2554 ATGCAT ATGCA^T
HhaI 3478 GCGC GCG^C
Hin1I 634 GACGTC G[GA]^CG[CT]C
HinP1I 3478 GCGC G^CGC
HincII 2213 GTTGAC GT[CT]^[GA]AC
HindII 2213 GTTGAC GT[CT]^[GA]AC
Ksp632I 1406 CTCTTC CTCTTC(1/4)
NaeI 1722 GCCGGC GCC^GGC
NgoMIV 1722 GCCGGC G^CCGGC
NheI 2854 GCTAGC G^CTAGC
NsiI 2554 ATGCAT ATGCA^T
PflFI 1203 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3750 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 630 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2971 ATTAAT AT^TAAT
Psp1406I 2314 AACGTT AA^CGTT
ScaI 1850 AGTACT AGT^ACT
SpeI 231 ACTAGT A^CTAGT
SspI 1475 AATATT AAT^ATT
Tth111I 1203 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3750 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3648 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 2950 GGTACC G^GTACC
3075 GGTACC
AccI 287 GTAGAC GT^[AC][GT]AC
3268 GTAGAC
AflII 1171 CTTAAG C^TTAAG
1177 CTTAAG
AflIII 2773 ACATGT A^C[GA][CT]GT
3257 ACATGT
Alw44I 593 GTGCAC G^TGCAC
2777 GTGCAC
ApaLI 593 GTGCAC G^TGCAC
2777 GTGCAC
Asp700I 1532 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3419 GAAGAATTTC
Asp718I 2950 GGTACC G^GTACC
3075 GGTACC
BcgI 1782 CGAGCAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
959 GCATAGACCTCG
BclI 1449 TGATCA T^GATCA
3149 TGATCA
BcnI 1757 CCCGG CC^[GC]GG
1763 CCCGG
BfrI 1171 CTTAAG C^TTAAG
1177 CTTAAG
BglII 580 AGATCT A^GATCT
3411 AGATCT
BsaI 2578 GAGACC GGTCTC(1/5)
216 GGTCTC
BspLU11I 2773 ACATGT A^CATGT
3257 ACATGT
BspMI 898 ACCTGC ACCTGC(4/8)
781 GCAGGT
BsrDI 3225 GCAATG GCAATG(2/0)
3847 GCAATG
EcoRI 1052 GAATTC G^AATTC
1543 GAATTC
FbaI 1449 TGATCA T^GATCA
3149 TGATCA
KpnI 2950 GGTACC GGTAC^C
3075 GGTACC
NciI 1757 CCCGG CC^[GC]GG
1763 CCCGG
SexAI 1667 ACCTGGT A^CC[AT]GGT
2843 ACCTGGT
XmnI 1532 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3419 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII