##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AocI ApaI AscI AseI AsnI
Asp718I AviII AvrII BbeI BfrI
BlnI BsiEI BsiWI BsrBI BssHII
Bst1107I BstBI BstZ17I Bsu36I DraI
DraIII EagI EclXI Eco52I Eco81I
EcoNI EcoT22I FseI FspI HpaI
KasI KpnI KspI MfeI MluI
MunI NarI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 2894 GGCGTC G[GA]^CG[CT]C
AflIII 4491 ACATGT A^C[GA][CT]GT
AhdI 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3783 AGCGCT AGC^GCT
AspEI 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3568 GGATCC G^GATCC
BbrPI 115 CACGTG CAC^GTG
BcgI 2568 GCAATTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 195 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 115 CACGTG [CT]AC^GT[GA]
BsaHI 2894 GGCGTC G[GA]^CG[CT]C
BspLU11I 4491 ACATGT A^CATGT
BsrDI 2497 CATTGC GCAATG(2/0)
CpoI 3375 CGGACCG CG^G[AT]CCG
DraII 2617 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3783 AGCGCT AGC^GCT
EcoO109I 2617 AGGACCC [GA]G^G[ACGT]CC[CT]
Hin1I 2894 GGCGTC G[GA]^CG[CT]C
HincII 3104 GTCAAC GT[CT]^[GA]AC
HindII 3104 GTCAAC GT[CT]^[GA]AC
NaeI 293 GCCGGC GCC^GGC
NgoMIV 293 GCCGGC G^CCGGC
NspI 4491 ACATGT [GA]CATG^[CT]
PflFI 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 115 CACGTG CAC^GTG
PmlI 115 CACGTG CAC^GTG
PpuMI 2617 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 3578 CAGCTG CAG^CTG
RsrII 3375 CGGACCG CG^G[AT]CCG
SapI 4326 GAAGAGC GCTCTTC(1/4)
SmaI 797 CCCGGG CCC^GGG
SmlI 3247 CTCAAG C^T[CT][GA]AG
SpeI 407 ACTAGT A^CTAGT
StuI 4618 AGGCCT AGG^CCT
Tth111I 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 797 CCCGGG C^CCGGG
XmaI 797 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 647 CGCG CG^CG
2291 CGCG
AclI 437 AACGTT AA^CGTT
2815 AACGTT
Asp700I 1405 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4083 GAATCCCTTC
BclI 2506 TGATCA T^GATCA
4244 TGATCA
BlpI 2087 GCTGAGC GC^T[ACGT]AGC
2194 GCTGAGC
Bpu1102I 2087 GCTGAGC GC^T[ACGT]AGC
2194 GCTGAGC
BsmBI 459 GAGACG CGTCTC(1/5)
2024 GAGACG
Bsp1407I 3759 TGTACA T^GTACA
4548 TGTACA
BspHI 1989 TCATGA T^CATGA
2842 TCATGA
BsrGI 3759 TGTACA T^GTACA
4548 TGTACA
BssSI 706 CACGAG CACGAG(-5/-1)
3341 CACGAG
BstUI 647 CGCG CG^CG
2291 CGCG
CelII 2087 GCTGAGC GC^T[ACGT]AGC
2194 GCTGAGC
DrdI 2762 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2888 GACCACGGCGTC
EcoRV 508 GATATC GAT^ATC
3026 GATATC
EcoT14I 1171 CCAAGG C^C[AT][AT]GG
2614 CCAAGG
FbaI 2506 TGATCA T^GATCA
4244 TGATCA
HaeII 320 AGCGCC [GA]GCGC^[CT]
3783 AGCGCT
HindIII 1073 AAGCTT A^AGCTT
4659 AAGCTT
MvnI 647 CGCG CG^CG
2291 CGCG
NdeI 1227 CATATG CA^TATG
2502 CATATG
Psp1406I 437 AACGTT AA^CGTT
2815 AACGTT
RcaI 1989 TCATGA T^CATGA
2842 TCATGA
SexAI 165 ACCAGGT A^CC[AT]GGT
963 ACCAGGT
SspBI 3759 TGTACA T^GTACA
4548 TGTACA
SspI 4476 AATATT AAT^ATT
4685 AATATT
StyI 1171 CCAAGG C^C[AT][AT]GG
2614 CCAAGG
XcmI 2910 CCACTGGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3294 CCACGCTGCGTGTGG
XmnI 1405 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4083 GAATCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I ClaI DdeI
DpnII DsaI EaeI EarI EcoO65I
EcoRI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII