################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccI AflII AgeI AocI ApaI AscI AseI AsnI Asp718I AviII AvrII BbeI BfrI BlnI BsiEI BsiWI BsrBI BssHII Bst1107I BstBI BstZ17I Bsu36I DraI DraIII EagI EclXI Eco52I Eco81I EcoNI EcoT22I FseI FspI HpaI KasI KpnI KspI MfeI MluI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcyI 2894 GGCGTC G[GA]^CG[CT]C AflIII 4491 ACATGT A^C[GA][CT]GT AhdI 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3783 AGCGCT AGC^GCT AspEI 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3568 GGATCC G^GATCC BbrPI 115 CACGTG CAC^GTG BcgI 2568 GCAATTACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 195 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 115 CACGTG [CT]AC^GT[GA] BsaHI 2894 GGCGTC G[GA]^CG[CT]C BspLU11I 4491 ACATGT A^CATGT BsrDI 2497 CATTGC GCAATG(2/0) CpoI 3375 CGGACCG CG^G[AT]CCG DraII 2617 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 4390 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3783 AGCGCT AGC^GCT EcoO109I 2617 AGGACCC [GA]G^G[ACGT]CC[CT] Hin1I 2894 GGCGTC G[GA]^CG[CT]C HincII 3104 GTCAAC GT[CT]^[GA]AC HindII 3104 GTCAAC GT[CT]^[GA]AC NaeI 293 GCCGGC GCC^GGC NgoMIV 293 GCCGGC G^CCGGC NspI 4491 ACATGT [GA]CATG^[CT] PflFI 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 115 CACGTG CAC^GTG PmlI 115 CACGTG CAC^GTG PpuMI 2617 AGGACCC [GA]G^G[AT]CC[CT] PvuII 3578 CAGCTG CAG^CTG RsrII 3375 CGGACCG CG^G[AT]CCG SapI 4326 GAAGAGC GCTCTTC(1/4) SmaI 797 CCCGGG CCC^GGG SmlI 3247 CTCAAG C^T[CT][GA]AG SpeI 407 ACTAGT A^CTAGT StuI 4618 AGGCCT AGG^CCT Tth111I 1844 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 797 CCCGGG C^CCGGG XmaI 797 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 647 CGCG CG^CG 2291 CGCG AclI 437 AACGTT AA^CGTT 2815 AACGTT Asp700I 1405 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4083 GAATCCCTTC BclI 2506 TGATCA T^GATCA 4244 TGATCA BlpI 2087 GCTGAGC GC^T[ACGT]AGC 2194 GCTGAGC Bpu1102I 2087 GCTGAGC GC^T[ACGT]AGC 2194 GCTGAGC BsmBI 459 GAGACG CGTCTC(1/5) 2024 GAGACG Bsp1407I 3759 TGTACA T^GTACA 4548 TGTACA BspHI 1989 TCATGA T^CATGA 2842 TCATGA BsrGI 3759 TGTACA T^GTACA 4548 TGTACA BssSI 706 CACGAG CACGAG(-5/-1) 3341 CACGAG BstUI 647 CGCG CG^CG 2291 CGCG CelII 2087 GCTGAGC GC^T[ACGT]AGC 2194 GCTGAGC DrdI 2762 GACATTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2888 GACCACGGCGTC EcoRV 508 GATATC GAT^ATC 3026 GATATC EcoT14I 1171 CCAAGG C^C[AT][AT]GG 2614 CCAAGG FbaI 2506 TGATCA T^GATCA 4244 TGATCA HaeII 320 AGCGCC [GA]GCGC^[CT] 3783 AGCGCT HindIII 1073 AAGCTT A^AGCTT 4659 AAGCTT MvnI 647 CGCG CG^CG 2291 CGCG NdeI 1227 CATATG CA^TATG 2502 CATATG Psp1406I 437 AACGTT AA^CGTT 2815 AACGTT RcaI 1989 TCATGA T^CATGA 2842 TCATGA SexAI 165 ACCAGGT A^CC[AT]GGT 963 ACCAGGT SspBI 3759 TGTACA T^GTACA 4548 TGTACA SspI 4476 AATATT AAT^ATT 4685 AATATT StyI 1171 CCAAGG C^C[AT][AT]GG 2614 CCAAGG XcmI 2910 CCACTGGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3294 CCACGCTGCGTGTGG XmnI 1405 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4083 GAATCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccIII AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I ClaI DdeI DpnII DsaI EaeI EarI EcoO65I EcoRI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII