##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI BamHI BbeI BcgI
BfrI BsaHI BsiWI BsmI BspDI
BspHI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DraIII DrdI Eco47III EcoRI
EcoT22I FseI HgaI Hin1I HindIII
HpaI KasI KspI MfeI MluI
MunI MvnI NarI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 4030 GGTACC G^GTACC
AccIII 882 TCCGGA T^CCGGA
AcsI 187 AAATTC [GA]^AATT[CT]
AhdI 1412 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 187 AAATTC [GA]^AATT[CT]
Asp700I 3370 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4030 GGTACC G^GTACC
AspEI 1412 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3307 CCTAGG C^CTAGG
BbrPI 3206 CACGTG CAC^GTG
BciVI 597 GGATAC GTATCC(6/5)
BclI 95 TGATCA T^GATCA
BglII 3806 AGATCT A^GATCT
BlnI 3307 CCTAGG C^CTAGG
BsaAI 3206 CACGTG [CT]AC^GT[GA]
BseAI 882 TCCGGA T^CCGGA
Bsp1407I 1424 TGTACA T^GTACA
BspEI 882 TCCGGA T^CCGGA
BspLU11I 107 ACATGT A^CATGT
BsrBI 1558 CCGCTC CCGCTC(-3/-3)
BsrGI 1424 TGTACA T^GTACA
BstEII 3225 GGTTACC G^GT[ACGT]ACC
BstPI 3225 GGTTACC G^GT[ACGT]ACC
DsaI 817 CCATGG C^C[GA][CT]GG
EagI 953 CGGCCG C^GGCCG
Eam1105I 1412 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 953 CGGCCG C^GGCCG
Eco52I 953 CGGCCG C^GGCCG
EcoO65I 3225 GGTTACC G^GT[ACGT]ACC
EcoRV 2564 GATATC GAT^ATC
FbaI 95 TGATCA T^GATCA
HaeII 1896 AGCGCC [GA]GCGC^[CT]
KpnI 4030 GGTACC GGTAC^C
MroI 882 TCCGGA T^CCGGA
MseI 3734 TTAA T^TAA
NaeI 1845 GCCGGC GCC^GGC
NcoI 817 CCATGG C^CATGG
NdeI 3886 CATATG CA^TATG
NgoMIV 1845 GCCGGC G^CCGGC
PmaCI 3206 CACGTG CAC^GTG
PmlI 3206 CACGTG CAC^GTG
PshAI 2603 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 737 GTCGAC G^TCGAC
ScaI 2516 AGTACT AGT^ACT
SexAI 3854 ACCTGGT A^CC[AT]GGT
SmaI 2034 CCCGGG CCC^GGG
SphI 1266 GCATGC GCATG^C
SspBI 1424 TGTACA T^GTACA
Tru9I 3734 TTAA T^TAA
XmaCI 2034 CCCGGG C^CCGGG
XmaI 2034 CCCGGG C^CCGGG
XmnI 3370 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 737 GTCGAC GT^[AC][GT]AC
959 GTAGAC
AspI 2026 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3270 GACATGGTC
AvaI 1944 CTCGGG C^[CT]CG[GA]G
2034 CCCGGG
AviII 3788 TGCGCA TGC^GCA
3890 TGCGCA
BbvCI 1295 GCTGAGG CCTCAGC(-5/-2)
1694 GCTGAGG
BsaBI 886 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3233 GATGCAGATC
BsiEI 953 CGGCCG CG[GA][CT]^CG
2060 CGGTCG
BsmBI 1794 GAGACG CGTCTC(1/5)
1912 GAGACG
BsoBI 1944 CTCGGG C^[CT]CG[GA]G
2034 CCCGGG
BspMI 2793 ACCTGC ACCTGC(4/8)
1839 GCAGGT
BsrDI 349 CATTGC GCAATG(2/0)
911 CATTGC
BsrFI 955 GCCGGT [GA]^CCGG[CT]
1845 GCCGGC
Cfr10I 955 GCCGGT [GA]^CCGG[CT]
1845 GCCGGC
FspI 3788 TGCGCA TGC^GCA
3890 TGCGCA
HincII 737 GTCGAC GT[CT]^[GA]AC
3052 GTTGAC
HindII 737 GTCGAC GT[CT]^[GA]AC
3052 GTTGAC
MamI 886 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3233 GATGCAGATC
NheI 3597 GCTAGC G^CTAGC
3953 GCTAGC
PflFI 2026 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3270 GACATGGTC
SacI 668 GAGCTC GAGCT^C
2422 GAGCTC
SbfI 2794 CCTGCAGG CCTGCA^GG
3513 CCTGCAGG
SfiI 2082 GGCCACGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3111 GGCCCCAGAGGCC
Sse8387I 2794 CCTGCAGG CCTGCA^GG
3513 CCTGCAGG
Tth111I 2026 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3270 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI ApaI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII