##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AviII BbeI
BcgI BciVI BfrI BsaHI BsaWI
BseAI BsiWI BspEI BspLU11I BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI CpoI DraI DrdI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HindIII KasI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3546 GGTACC G^GTACC
AcsI 1951 GAATTT [GA]^AATT[CT]
AhdI 497 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 816 GGGCCC GGGCC^C
ApoI 1951 GAATTT [GA]^AATT[CT]
Asp718I 3546 GGTACC G^GTACC
AspEI 497 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2180 TGGCCA TGG^CCA
BbrPI 2850 CACGTG CAC^GTG
BclI 4411 TGATCA T^GATCA
BsaBI 4526 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1251 GAGACC GGTCTC(1/5)
BsiEI 727 CGGCCG CG[GA][CT]^CG
BspDI 1325 ATCGAT AT^CGAT
BspHI 2091 TCATGA T^CATGA
BspMI 1268 ACCTGC ACCTGC(4/8)
BsrFI 725 ACCGGC [GA]^CCGG[CT]
Bst1107I 1014 GTATAC GTA^TAC
BstZ17I 1014 GTATAC GTA^TAC
Cfr10I 725 ACCGGC [GA]^CCGG[CT]
ClaI 1325 ATCGAT AT^CGAT
DraIII 1457 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 727 CGGCCG C^GGCCG
Eam1105I 497 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 727 CGGCCG C^GGCCG
Eco52I 727 CGGCCG C^GGCCG
FbaI 4411 TGATCA T^GATCA
KpnI 3546 GGTACC GGTAC^C
MamI 4526 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2888 CAATTG C^AATTG
MluNI 2180 TGGCCA TGG^CCA
MscI 2180 TGGCCA TGG^CCA
MunI 2888 CAATTG C^AATTG
NheI 1414 GCTAGC G^CTAGC
PmaCI 2850 CACGTG CAC^GTG
PmlI 2850 CACGTG CAC^GTG
RcaI 2091 TCATGA T^CATGA
SacI 1994 GAGCTC GAGCT^C
SexAI 1254 ACCAGGT A^CC[AT]GGT
SphI 1451 GCATGC GCATG^C
SspI 1237 AATATT AAT^ATT
XbaI 3727 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1014 GTATAC GT^[AC][GT]AC
4498 GTCTAC
AflIII 2649 ACACGT A^C[GA][CT]GT
4379 ACGTGT
Aor51HI 1856 AGCGCT AGC^GCT
3300 AGCGCT
AspI 607 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1253 GACCAGGTC
AvrII 3810 CCTAGG C^CTAGG
3824 CCTAGG
BbvCI 448 CCTCAGC CCTCAGC(-5/-2)
3069 CCTCAGC
BglI 46 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2360 GCCAGAGGGGC
BlnI 3810 CCTAGG C^CTAGG
3824 CCTAGG
BsaAI 2850 CACGTG [CT]AC^GT[GA]
4378 TACGTG
BsmBI 1431 CGTCTC CGTCTC(1/5)
1137 GAGACG
Bsp1407I 657 TGTACA T^GTACA
3600 TGTACA
BsrBI 1486 GAGCGG CCGCTC(-3/-3)
2827 GAGCGG
BsrGI 657 TGTACA T^GTACA
3600 TGTACA
Eco47III 1856 AGCGCT AGC^GCT
3300 AGCGCT
HgaI 1139 GACGC GACGC(5/10)
465 GCGTC
HpaI 223 GTTAAC GTT^AAC
2131 GTTAAC
PaeR7I 578 CTCGAG C^TCGAG
3260 CTCGAG
PflFI 607 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1253 GACCAGGTC
SapI 3610 GAAGAGC GCTCTTC(1/4)
1418 GCTCTTC
SspBI 657 TGTACA T^GTACA
3600 TGTACA
StuI 1646 AGGCCT AGG^CCT
2744 AGGCCT
Tth111I 607 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1253 GACCAGGTC
XhoI 578 CTCGAG C^TCGAG
3260 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII