##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaI ApaLI AscI AvrII BalI
BamHI BbrPI BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
DraIII DrdI EcoNI EcoRI FseI
HindIII KspI MfeI MluI MluNI
MroI MscI MunI NruI NspV
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
StuI SwaI Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 963 GTCTAC GT^[AC][GT]AC
AhdI 83 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 4685 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 164 AGCGCT AGC^GCT
AseI 4328 ATTAAT AT^TAAT
AsnI 4328 ATTAAT AT^TAAT
Asp700I 2626 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 83 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1544 TGCGCA TGC^GCA
BbsI 1081 GTCTTC GAAGAC(2/6)
BpuAI 1081 GTCTTC GAAGAC(2/6)
BsaAI 3966 CACGTA [CT]AC^GT[GA]
BsaWI 138 TCCGGT [AT]^CCGG[AT]
BsgI 2566 CTGCAC GTGCAG(16/14)
BspHI 3520 TCATGA T^CATGA
BspLU11I 3750 ACATGT A^CATGT
BspMI 575 ACCTGC ACCTGC(4/8)
BsrDI 2724 CATTGC GCAATG(2/0)
BsrFI 424 GCCGGC [GA]^CCGG[CT]
BssSI 765 CTCGTG CACGAG(-5/-1)
BstEII 197 GGTCACC G^GT[ACGT]ACC
BstPI 197 GGTCACC G^GT[ACGT]ACC
Cfr10I 424 GCCGGC [GA]^CCGG[CT]
CpoI 251 CGGACCG CG^G[AT]CCG
EagI 457 CGGCCG C^GGCCG
Eam1105I 83 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 457 CGGCCG C^GGCCG
Eco47III 164 AGCGCT AGC^GCT
Eco52I 457 CGGCCG C^GGCCG
EcoO65I 197 GGTCACC G^GT[ACGT]ACC
FspI 1544 TGCGCA TGC^GCA
HpaI 1028 GTTAAC GTT^AAC
NaeI 424 GCCGGC GCC^GGC
NdeI 4005 CATATG CA^TATG
NgoMIV 424 GCCGGC G^CCGGC
NheI 2088 GCTAGC G^CTAGC
NotI 456 GCGGCCGC GC^GGCCGC
PacI 1950 TTAATTAA TTAAT^TAA
PflFI 257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4097 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1362 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4328 ATTAAT AT^TAAT
RcaI 3520 TCATGA T^CATGA
RsrII 251 CGGACCG CG^G[AT]CCG
SexAI 4905 ACCAGGT A^CC[AT]GGT
SpeI 4989 ACTAGT A^CTAGT
SphI 1492 GCATGC GCATG^C
Tth111I 257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2871 TCTAGA T^CTAGA
XmnI 2626 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1413 GGTACC G^GTACC
3583 GGTACC
AccII 95 CGCG CG^CG
527 CGCG
AflII 1185 CTTAAG C^TTAAG
3369 CTTAAG
AflIII 3750 ACATGT A^C[GA][CT]GT
3965 ACACGT
AocI 2157 CCTGAGG CC^T[ACGT]AGG
3437 CCTCAGG
Asp718I 1413 GGTACC G^GTACC
3583 GGTACC
AvaI 104 CTCGGG C^[CT]CG[GA]G
389 CTCGGG
BanII 150 GAGCCC G[GA]GC[CT]^C
229 GAGCCC
BcgI 512 CGACTCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1976 GCAGTTGGTTCG
BclI 1907 TGATCA T^GATCA
3296 TGATCA
BfrI 1185 CTTAAG C^TTAAG
3369 CTTAAG
BglI 101 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
828 GCCAAAGAGGC
BpmI 879 CTGGAG CTGGAG(16/14)
4890 CTGGAG
BsaBI 1214 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2166 GATCAACATC
BsaI 882 GAGACC GGTCTC(1/5)
1360 GAGACC
BsoBI 104 CTCGGG C^[CT]CG[GA]G
389 CTCGGG
BstUI 95 CGCG CG^CG
527 CGCG
Bsu36I 2157 CCTGAGG CC^T[ACGT]AGG
3437 CCTCAGG
DraI 1206 TTTAAA TTT^AAA
4409 TTTAAA
DraII 3222 AGGCCCC [GA]G^G[ACGT]CC[CT]
4097 AGGACCT
EaeI 457 CGGCCG [CT]^GGCC[GA]
706 CGGCCA
Eco81I 2157 CCTGAGG CC^T[ACGT]AGG
3437 CCTCAGG
EcoO109I 3222 AGGCCCC [GA]G^G[ACGT]CC[CT]
4097 AGGACCT
EcoRV 867 GATATC GAT^ATC
2643 GATATC
EcoT22I 1145 ATGCAT ATGCA^T
3642 ATGCAT
FbaI 1907 TGATCA T^GATCA
3296 TGATCA
HgaI 96 GCGTC GACGC(5/10)
502 GCGTC
HincII 1028 GTTAAC GT[CT]^[GA]AC
3385 GTTGAC
HindII 1028 GTTAAC GT[CT]^[GA]AC
3385 GTTGAC
KpnI 1413 GGTACC GGTAC^C
3583 GGTACC
MaeII 1191 ACGT A^CGT
3967 ACGT
MamI 1214 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2166 GATCAACATC
MslI 4312 CATAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4529 CACTCTGGTG
MvnI 95 CGCG CG^CG
527 CGCG
NcoI 1918 CCATGG C^CATGG
2375 CCATGG
NsiI 1145 ATGCAT ATGCA^T
3642 ATGCAT
SapI 873 GAAGAGC GCTCTTC(1/4)
1989 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BbeI BbvCI BbvI BciVI BcnI
BfaI BglII BmyI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII