##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BbeI BbvCI BcgI
BcnI BglI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FseI
FspI HaeII HapII HhaI HinP1I
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AccI 1589 GTCTAC GT^[AC][GT]AC
AciI 435 CCGC CCGC(-3/-1)
AcyI 430 GACGCC G[GA]^CG[CT]C
AflII 2842 CTTAAG C^TTAAG
Alw44I 2194 GTGCAC G^TGCAC
AlwI 886 GGATC GGATC(4/5)
AocI 3929 CCTGAGG CC^T[ACGT]AGG
ApaLI 2194 GTGCAC G^TGCAC
Asp700I 816 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 105 CTCGAG C^[CT]CG[GA]G
AvrII 876 CCTAGG C^CTAGG
BanI 790 GGCACC G^G[CT][GA]CC
BbrPI 297 CACGTG CAC^GTG
BbsI 653 GAAGAC GAAGAC(2/6)
BclI 1174 TGATCA T^GATCA
BfrI 2842 CTTAAG C^TTAAG
BlnI 876 CCTAGG C^CTAGG
BlpI 1569 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1569 GCTGAGC GC^T[ACGT]AGC
BpuAI 653 GAAGAC GAAGAC(2/6)
BsaAI 297 CACGTG [CT]AC^GT[GA]
BsaBI 1547 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 430 GACGCC G[GA]^CG[CT]C
BsmBI 20 GAGACG CGTCTC(1/5)
BsoBI 105 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1370 TGTACA T^GTACA
BsrGI 1370 TGTACA T^GTACA
BssSI 2655 CACGAG CACGAG(-5/-1)
BstEII 2500 GGTCACC G^GT[ACGT]ACC
BstPI 2500 GGTCACC G^GT[ACGT]ACC
BstXI 173 CCAAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3929 CCTGAGG CC^T[ACGT]AGG
CelII 1569 GCTGAGC GC^T[ACGT]AGC
DraIII 3077 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1349 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3929 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2500 GGTCACC G^GT[ACGT]ACC
EcoT22I 384 ATGCAT ATGCA^T
FauI 434 CCCGC CCCGC(4/6)
FbaI 1174 TGATCA T^GATCA
HgaI 430 GACGC GACGC(5/10)
Hin1I 430 GACGCC G[GA]^CG[CT]C
HpaI 2261 GTTAAC GTT^AAC
MamI 1547 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 73 CAATTG C^AATTG
MspA1I 47 CAGCTG C[AC]G^C[GT]G
MunI 73 CAATTG C^AATTG
NheI 3819 GCTAGC G^CTAGC
NsiI 384 ATGCAT ATGCA^T
PaeR7I 105 CTCGAG C^TCGAG
PmaCI 297 CACGTG CAC^GTG
PmlI 297 CACGTG CAC^GTG
PvuII 47 CAGCTG CAG^CTG
SacI 1572 GAGCTC GAGCT^C
SapI 1102 GCTCTTC GCTCTTC(1/4)
SbfI 2177 CCTGCAGG CCTGCA^GG
ScaI 563 AGTACT AGT^ACT
SexAI 1183 ACCTGGT A^CC[AT]GGT
SphI 2045 GCATGC GCATG^C
Sse8387I 2177 CCTGCAGG CCTGCA^GG
SspBI 1370 TGTACA T^GTACA
StuI 3557 AGGCCT AGG^CCT
TaqI 106 TCGA T^CGA
TthHB8I 106 TCGA T^CGA
XhoI 105 CTCGAG C^TCGAG
XmnI 816 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
BstAPI 43 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2949 GCATCATCTGC
DraI 98 TTTAAA TTT^AAA
227 TTTAAA
MaeII 298 ACGT A^CGT
3633 ACGT
NdeI 667 CATATG CA^TATG
3168 CATATG
PflMI 248 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGAGAGTGG
SspI 707 AATATT AAT^ATT
3303 AATATT
Van91I 248 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGAGAGTGG
XcmI 900 CCAAACAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1238 CCAACCAGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwNI
ApoI AspHI AvaII BalI BanII
BbvI BciVI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII