##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BbsI BcgI BclI BfrI BglI
BpuAI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 2417 GAATTC [GA]^AATT[CT]
AflIII 3702 ACGTGT A^C[GA][CT]GT
Alw44I 2957 GTGCAC G^TGCAC
ApaLI 2957 GTGCAC G^TGCAC
ApoI 2417 GAATTC [GA]^AATT[CT]
BbeI 2484 GGCGCC GGCGC^C
BbrPI 3701 CACGTG CAC^GTG
BciVI 685 GGATAC GTATCC(6/5)
BsaAI 3701 CACGTG [CT]AC^GT[GA]
BsaWI 2814 ACCGGA [AT]^CCGG[AT]
BsiEI 355 CGATCG CG[GA][CT]^CG
Bsp1407I 1521 TGTACA T^GTACA
BsrGI 1521 TGTACA T^GTACA
DraI 562 TTTAAA TTT^AAA
DrdI 4231 GACCACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 420 CCACGG C^C[GA][CT]GG
EcoRI 2417 GAATTC G^AATTC
EcoRV 2477 GATATC GAT^ATC
HindIII 2653 AAGCTT A^AGCTT
HpaI 3994 GTTAAC GTT^AAC
KasI 2484 GGCGCC G^GCGCC
NarI 2484 GGCGCC GG^CGCC
NheI 1676 GCTAGC G^CTAGC
PaeR7I 971 CTCGAG C^TCGAG
PflMI 3788 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3701 CACGTG CAC^GTG
PmlI 3701 CACGTG CAC^GTG
PshAI 1178 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 355 CGATCG CGAT^CG
ScaI 4339 AGTACT AGT^ACT
SexAI 1247 ACCTGGT A^CC[AT]GGT
SfoI 2484 GGCGCC GGC^GCC
SspBI 1521 TGTACA T^GTACA
Van91I 3788 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 971 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1609 GGCGTC G[GA]^CG[CT]C
2484 GGCGCC
AfaI 1522 GTAC GT^AC
4340 GTAC
AhdI 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2945 GACTGCGGGTC
Asp700I 629 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3756 GAAGTGATTC
AspEI 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2945 GACTGCGGGTC
AvrII 3912 CCTAGG C^CTAGG
4114 CCTAGG
BamHI 798 GGATCC G^GATCC
2179 GGATCC
BlnI 3912 CCTAGG C^CTAGG
4114 CCTAGG
BsaHI 1609 GGCGTC G[GA]^CG[CT]C
2484 GGCGCC
BsrDI 3144 CATTGC GCAATG(2/0)
599 GCAATG
BstAPI 2246 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3237 GCAGCGAGTGC
BstXI 1774 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4213 CCAGGTGGTTGG
DraIII 421 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2247 CACCCAGTG
Eam1105I 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2945 GACTGCGGGTC
HgaI 2848 GACGC GACGC(5/10)
1610 GCGTC
Hin1I 1609 GGCGTC G[GA]^CG[CT]C
2484 GGCGCC
HincII 3994 GTTAAC GT[CT]^[GA]AC
4328 GTCAAC
HindII 3994 GTTAAC GT[CT]^[GA]AC
4328 GTCAAC
PpuMI 1062 AGGACCT [GA]G^G[AT]CC[CT]
3043 GGGTCCC
RsaI 1522 GTAC GT^AC
4340 GTAC
SacI 453 GAGCTC GAGCT^C
1999 GAGCTC
SapI 2001 GCTCTTC GCTCTTC(1/4)
3359 GCTCTTC
SphI 3088 GCATGC GCATG^C
4086 GCATGC
XcmI 2662 CCAGGAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3620 CCAGCTCTGGAGTGG
XmnI 629 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3756 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII