################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII BbsI BcgI BclI BfrI BglI BpuAI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 2417 GAATTC [GA]^AATT[CT] AflIII 3702 ACGTGT A^C[GA][CT]GT Alw44I 2957 GTGCAC G^TGCAC ApaLI 2957 GTGCAC G^TGCAC ApoI 2417 GAATTC [GA]^AATT[CT] BbeI 2484 GGCGCC GGCGC^C BbrPI 3701 CACGTG CAC^GTG BciVI 685 GGATAC GTATCC(6/5) BsaAI 3701 CACGTG [CT]AC^GT[GA] BsaWI 2814 ACCGGA [AT]^CCGG[AT] BsiEI 355 CGATCG CG[GA][CT]^CG Bsp1407I 1521 TGTACA T^GTACA BsrGI 1521 TGTACA T^GTACA DraI 562 TTTAAA TTT^AAA DrdI 4231 GACCACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 420 CCACGG C^C[GA][CT]GG EcoRI 2417 GAATTC G^AATTC EcoRV 2477 GATATC GAT^ATC HindIII 2653 AAGCTT A^AGCTT HpaI 3994 GTTAAC GTT^AAC KasI 2484 GGCGCC G^GCGCC NarI 2484 GGCGCC GG^CGCC NheI 1676 GCTAGC G^CTAGC PaeR7I 971 CTCGAG C^TCGAG PflMI 3788 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3701 CACGTG CAC^GTG PmlI 3701 CACGTG CAC^GTG PshAI 1178 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 355 CGATCG CGAT^CG ScaI 4339 AGTACT AGT^ACT SexAI 1247 ACCTGGT A^CC[AT]GGT SfoI 2484 GGCGCC GGC^GCC SspBI 1521 TGTACA T^GTACA Van91I 3788 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 971 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 1609 GGCGTC G[GA]^CG[CT]C 2484 GGCGCC AfaI 1522 GTAC GT^AC 4340 GTAC AhdI 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2945 GACTGCGGGTC Asp700I 629 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3756 GAAGTGATTC AspEI 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2945 GACTGCGGGTC AvrII 3912 CCTAGG C^CTAGG 4114 CCTAGG BamHI 798 GGATCC G^GATCC 2179 GGATCC BlnI 3912 CCTAGG C^CTAGG 4114 CCTAGG BsaHI 1609 GGCGTC G[GA]^CG[CT]C 2484 GGCGCC BsrDI 3144 CATTGC GCAATG(2/0) 599 GCAATG BstAPI 2246 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3237 GCAGCGAGTGC BstXI 1774 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4213 CCAGGTGGTTGG DraIII 421 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2247 CACCCAGTG Eam1105I 1244 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2945 GACTGCGGGTC HgaI 2848 GACGC GACGC(5/10) 1610 GCGTC Hin1I 1609 GGCGTC G[GA]^CG[CT]C 2484 GGCGCC HincII 3994 GTTAAC GT[CT]^[GA]AC 4328 GTCAAC HindII 3994 GTTAAC GT[CT]^[GA]AC 4328 GTCAAC PpuMI 1062 AGGACCT [GA]G^G[AT]CC[CT] 3043 GGGTCCC RsaI 1522 GTAC GT^AC 4340 GTAC SacI 453 GAGCTC GAGCT^C 1999 GAGCTC SapI 2001 GCTCTTC GCTCTTC(1/4) 3359 GCTCTTC SphI 3088 GCATGC GCATG^C 4086 GCATGC XcmI 2662 CCAGGAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3620 CCAGCTCTGGAGTGG XmnI 629 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3756 GAAGTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII