##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I ApaLI AscI Asp718I
BbeI BbrPI BclI BfrI BglII
BsaBI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraIII EcoRI EcoRV
EcoT22I FbaI FseI HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmlI PshAI Psp1406I PvuI
RcaI RsrII SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1361 GTCGAC GT^[AC][GT]AC
AflIII 2367 ACATGT A^C[GA][CT]GT
AocI 2063 CCTAAGG CC^T[ACGT]AGG
Aor51HI 4135 AGCGCT AGC^GCT
ApaI 3285 GGGCCC GGGCC^C
AseI 3841 ATTAAT AT^TAAT
AsnI 3841 ATTAAT AT^TAAT
AspI 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1560 TGCGCA TGC^GCA
AvrII 4378 CCTAGG C^CTAGG
BamHI 1822 GGATCC G^GATCC
BcgI 2349 GCATCTACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4378 CCTAGG C^CTAGG
BsaAI 2711 CACGTA [CT]AC^GT[GA]
BspLU11I 2367 ACATGT A^CATGT
BssSI 2073 CACGAG CACGAG(-5/-1)
Bsu36I 2063 CCTAAGG CC^T[ACGT]AGG
DrdI 4341 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4135 AGCGCT AGC^GCT
Eco81I 2063 CCTAAGG CC^T[ACGT]AGG
FspI 1560 TGCGCA TGC^GCA
HindIII 2646 AAGCTT A^AGCTT
KspI 13 CCGCGG CCGC^GG
NcoI 114 CCATGG C^CATGG
PaeR7I 1694 CTCGAG C^TCGAG
PflFI 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2146 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 520 GTTTAAAC GTTT^AAAC
PshBI 3841 ATTAAT AT^TAAT
SacII 13 CCGCGG CCGC^GG
SalI 1361 GTCGAC G^TCGAC
SexAI 231 ACCTGGT A^CC[AT]GGT
Tth111I 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2146 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2977 TCTAGA T^CTAGA
XhoI 1694 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 9 GACGCC G[GA]^CG[CT]C
134 GGCGTC
BbsI 1699 GAAGAC GAAGAC(2/6)
4230 GAAGAC
BglI 1382 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4040 GCCTGCTGGGC
BpuAI 1699 GAAGAC GAAGAC(2/6)
4230 GAAGAC
BsaHI 9 GACGCC G[GA]^CG[CT]C
134 GGCGTC
BsiEI 1608 CGGCCG CG[GA][CT]^CG
2237 CGGCCG
BsmBI 674 GAGACG CGTCTC(1/5)
953 GAGACG
BsrFI 3021 GCCGGC [GA]^CCGG[CT]
3132 GCCGGC
BstEII 235 GGTGACC G^GT[ACGT]ACC
2962 GGTGACC
BstPI 235 GGTGACC G^GT[ACGT]ACC
2962 GGTGACC
Cfr10I 3021 GCCGGC [GA]^CCGG[CT]
3132 GCCGGC
DraI 521 TTTAAA TTT^AAA
4286 TTTAAA
EagI 1608 CGGCCG C^GGCCG
2237 CGGCCG
EclXI 1608 CGGCCG C^GGCCG
2237 CGGCCG
Eco52I 1608 CGGCCG C^GGCCG
2237 CGGCCG
EcoO65I 235 GGTGACC G^GT[ACGT]ACC
2962 GGTGACC
Hin1I 9 GACGCC G[GA]^CG[CT]C
134 GGCGTC
NaeI 3021 GCCGGC GCC^GGC
3132 GCCGGC
NgoMIV 3021 GCCGGC G^CCGGC
3132 GCCGGC
NspI 2094 ACATGC [GA]CATG^[CT]
2367 ACATGT
SapI 1232 GAAGAGC GCTCTTC(1/4)
2104 GCTCTTC
ScaI 379 AGTACT AGT^ACT
1395 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI AlwI AlwNI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI