################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I ApaLI AscI Asp718I BbeI BbrPI BclI BfrI BglII BsaBI BseAI BsgI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmlI PshAI Psp1406I PvuI RcaI RsrII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 1361 GTCGAC GT^[AC][GT]AC AflIII 2367 ACATGT A^C[GA][CT]GT AocI 2063 CCTAAGG CC^T[ACGT]AGG Aor51HI 4135 AGCGCT AGC^GCT ApaI 3285 GGGCCC GGGCC^C AseI 3841 ATTAAT AT^TAAT AsnI 3841 ATTAAT AT^TAAT AspI 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1560 TGCGCA TGC^GCA AvrII 4378 CCTAGG C^CTAGG BamHI 1822 GGATCC G^GATCC BcgI 2349 GCATCTACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 4378 CCTAGG C^CTAGG BsaAI 2711 CACGTA [CT]AC^GT[GA] BspLU11I 2367 ACATGT A^CATGT BssSI 2073 CACGAG CACGAG(-5/-1) Bsu36I 2063 CCTAAGG CC^T[ACGT]AGG DrdI 4341 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4135 AGCGCT AGC^GCT Eco81I 2063 CCTAAGG CC^T[ACGT]AGG FspI 1560 TGCGCA TGC^GCA HindIII 2646 AAGCTT A^AGCTT KspI 13 CCGCGG CCGC^GG NcoI 114 CCATGG C^CATGG PaeR7I 1694 CTCGAG C^TCGAG PflFI 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2146 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 520 GTTTAAAC GTTT^AAAC PshBI 3841 ATTAAT AT^TAAT SacII 13 CCGCGG CCGC^GG SalI 1361 GTCGAC G^TCGAC SexAI 231 ACCTGGT A^CC[AT]GGT Tth111I 491 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2146 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2977 TCTAGA T^CTAGA XhoI 1694 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 9 GACGCC G[GA]^CG[CT]C 134 GGCGTC BbsI 1699 GAAGAC GAAGAC(2/6) 4230 GAAGAC BglI 1382 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4040 GCCTGCTGGGC BpuAI 1699 GAAGAC GAAGAC(2/6) 4230 GAAGAC BsaHI 9 GACGCC G[GA]^CG[CT]C 134 GGCGTC BsiEI 1608 CGGCCG CG[GA][CT]^CG 2237 CGGCCG BsmBI 674 GAGACG CGTCTC(1/5) 953 GAGACG BsrFI 3021 GCCGGC [GA]^CCGG[CT] 3132 GCCGGC BstEII 235 GGTGACC G^GT[ACGT]ACC 2962 GGTGACC BstPI 235 GGTGACC G^GT[ACGT]ACC 2962 GGTGACC Cfr10I 3021 GCCGGC [GA]^CCGG[CT] 3132 GCCGGC DraI 521 TTTAAA TTT^AAA 4286 TTTAAA EagI 1608 CGGCCG C^GGCCG 2237 CGGCCG EclXI 1608 CGGCCG C^GGCCG 2237 CGGCCG Eco52I 1608 CGGCCG C^GGCCG 2237 CGGCCG EcoO65I 235 GGTGACC G^GT[ACGT]ACC 2962 GGTGACC Hin1I 9 GACGCC G[GA]^CG[CT]C 134 GGCGTC NaeI 3021 GCCGGC GCC^GGC 3132 GCCGGC NgoMIV 3021 GCCGGC G^CCGGC 3132 GCCGGC NspI 2094 ACATGC [GA]CATG^[CT] 2367 ACATGT SapI 1232 GAAGAGC GCTCTTC(1/4) 2104 GCTCTTC ScaI 379 AGTACT AGT^ACT 1395 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcsI AfaI AhdI AluI AlwI AlwNI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI