##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BciVI BglI BglII BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BstAPI BstBI BstEII
BstPI BstUI CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NarI NdeI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 994 CTTAAG C^TTAAG
AseI 3753 ATTAAT AT^TAAT
AsnI 3753 ATTAAT AT^TAAT
BalI 2525 TGGCCA TGG^CCA
BbvCI 871 CCTCAGC CCTCAGC(-5/-2)
BcgI 3448 CGAGGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 582 TGATCA T^GATCA
BfrI 994 CTTAAG C^TTAAG
BsmI 978 GCATTC GAATGC(1/-1)
Bsp1407I 5017 TGTACA T^GTACA
BspLU11I 4156 ACATGT A^CATGT
BsrFI 2423 GCCGGC [GA]^CCGG[CT]
BsrGI 5017 TGTACA T^GTACA
BssSI 2486 CTCGTG CACGAG(-5/-1)
Bst1107I 239 GTATAC GTA^TAC
BstXI 678 CCAGGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 239 GTATAC GTA^TAC
CfoI 3998 GCGC GCG^C
Cfr10I 2423 GCCGGC [GA]^CCGG[CT]
DraIII 2918 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 582 TGATCA T^GATCA
HgaI 4014 GACGC GACGC(5/10)
HhaI 3998 GCGC GCG^C
HinP1I 3998 GCGC G^CGC
HincII 4082 GTTAAC GT[CT]^[GA]AC
HindII 4082 GTTAAC GT[CT]^[GA]AC
HpaI 4082 GTTAAC GTT^AAC
MluNI 2525 TGGCCA TGG^CCA
MscI 2525 TGGCCA TGG^CCA
MslI 3583 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2423 GCCGGC GCC^GGC
NcoI 233 CCATGG C^CATGG
NgoMIV 2423 GCCGGC G^CCGGC
PaeR7I 1526 CTCGAG C^TCGAG
PshBI 3753 ATTAAT AT^TAAT
SacI 4024 GAGCTC GAGCT^C
SapI 2335 GAAGAGC GCTCTTC(1/4)
ScaI 5038 AGTACT AGT^ACT
SmaI 3519 CCCGGG CCC^GGG
SspBI 5017 TGTACA T^GTACA
SspI 5031 AATATT AAT^ATT
XcmI 511 CCAAAGAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1526 CTCGAG C^TCGAG
XmaCI 3519 CCCGGG C^CCGGG
XmaI 3519 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1771 ACACGT A^C[GA][CT]GT
4156 ACATGT
AocI 1981 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
AvrII 133 CCTAGG C^CTAGG
1516 CCTAGG
BlnI 133 CCTAGG C^CTAGG
1516 CCTAGG
BsaAI 1421 CACGTA [CT]AC^GT[GA]
1772 CACGTA
BsaI 698 GAGACC GGTCTC(1/5)
3227 GAGACC
BseRI 838 CTCCTC GAGGAG(10/8)
2953 CTCCTC
BspHI 102 TCATGA T^CATGA
3822 TCATGA
BsrDI 3905 CATTGC GCAATG(2/0)
4772 GCAATG
Bsu36I 1981 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
DraI 3781 TTTAAA TTT^AAA
4412 TTTAAA
DrdI 1770 GACACGTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1966 GACCATCTTGTC
EaeI 2525 TGGCCA [CT]^GGCC[GA]
4552 CGGCCA
Eco81I 1981 CCTAAGG CC^T[ACGT]AGG
3281 CCTAAGG
EcoNI 1079 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2598 CCTGGGGAAGG
EcoT22I 4419 ATGCAT ATGCA^T
4649 ATGCAT
HindIII 821 AAGCTT A^AGCTT
2878 AAGCTT
MspA1I 530 CAGCTG C[AC]G^C[GT]G
3437 CAGCTG
NsiI 4419 ATGCAT ATGCA^T
4649 ATGCAT
PflMI 153 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3799 CCAGGATGTGG
PstI 1591 CTGCAG CTGCA^G
3853 CTGCAG
PvuII 530 CAGCTG CAG^CTG
3437 CAGCTG
RcaI 102 TCATGA T^CATGA
3822 TCATGA
SphI 2861 GCATGC GCATG^C
4651 GCATGC
Van91I 153 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3799 CCAGGATGTGG
XbaI 1036 TCTAGA T^CTAGA
4064 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI