################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AgeI Aor51HI AscI AseI AsnI Asp700I AviII BamHI BbrPI BcgI BclI BsiWI BsmBI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DrdI Eco47III EcoO65I EcoRI EcoT22I FbaI FseI FspI HpaI MfeI MluI MunI NaeI NgoMIV NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1539 GACGTC GACGT^C Acc65I 1850 GGTACC G^GTACC AccIII 1558 TCCGGA T^CCGGA AclI 716 AACGTT AA^CGTT AcsI 2312 AAATTT [GA]^AATT[CT] AflII 4349 CTTAAG C^TTAAG AflIII 3378 ACGTGT A^C[GA][CT]GT ApaI 1212 GGGCCC GGGCC^C ApoI 2312 AAATTT [GA]^AATT[CT] Asp718I 1850 GGTACC G^GTACC BalI 3549 TGGCCA TGG^CCA BbvCI 1050 CCTCAGC CCTCAGC(-5/-2) BfrI 4349 CTTAAG C^TTAAG BlpI 4384 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4384 GCTCAGC GC^T[ACGT]AGC BsaAI 813 TACGTG [CT]AC^GT[GA] BsaBI 778 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1558 TCCGGA T^CCGGA Bsp1407I 2588 TGTACA T^GTACA BspDI 1710 ATCGAT AT^CGAT BspEI 1558 TCCGGA T^CCGGA BsrFI 601 GCCGGT [GA]^CCGG[CT] BsrGI 2588 TGTACA T^GTACA CelII 4384 GCTCAGC GC^T[ACGT]AGC Cfr10I 601 GCCGGT [GA]^CCGG[CT] ClaI 1710 ATCGAT AT^CGAT EcoRV 3626 GATATC GAT^ATC HgaI 4479 GACGC GACGC(5/10) KpnI 1850 GGTACC GGTAC^C MamI 778 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3549 TGGCCA TGG^CCA MroI 1558 TCCGGA T^CCGGA MscI 3549 TGGCCA TGG^CCA NdeI 4108 CATATG CA^TATG NheI 2353 GCTAGC G^CTAGC NotI 48 GCGGCCGC GC^GGCCGC Psp1406I 716 AACGTT AA^CGTT SapI 4408 GCTCTTC GCTCTTC(1/4) SexAI 397 ACCAGGT A^CC[AT]GGT SspBI 2588 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 2779 GTCTAC GT^[AC][GT]AC 3758 GTAGAC Alw44I 3308 GTGCAC G^TGCAC 3530 GTGCAC AocI 3041 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG ApaLI 3308 GTGCAC G^TGCAC 3530 GTGCAC AspI 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2739 GACATGGTC BbeI 31 GGCGCC GGCGC^C 571 GGCGCC BbsI 2873 GTCTTC GAAGAC(2/6) 4047 GTCTTC BciVI 526 GTATCC GTATCC(6/5) 2439 GTATCC BglII 1576 AGATCT A^GATCT 3198 AGATCT BpuAI 2873 GTCTTC GAAGAC(2/6) 4047 GTCTTC BsmI 591 GAATGC GAATGC(1/-1) 4078 GCATTC BspHI 2922 TCATGA T^CATGA 4593 TCATGA BsrDI 1799 CATTGC GCAATG(2/0) 4508 GCAATG BssHII 549 GCGCGC G^CGCGC 551 GCGCGC BssSI 1440 CACGAG CACGAG(-5/-1) 3389 CACGAG BstAPI 598 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4399 GCATCGACTGC BstXI 1168 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1612 CCAGCTTTGTGG Bsu36I 3041 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG Eco81I 3041 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG HindIII 282 AAGCTT A^AGCTT 666 AAGCTT KasI 31 GGCGCC G^GCGCC 571 GGCGCC KspI 152 CCGCGG CCGC^GG 1596 CCGCGG MslI 1112 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4079 CATTCTTGTG NarI 31 GGCGCC GG^CGCC 571 GGCGCC NspI 434 GCATGT [GA]CATG^[CT] 2377 GCATGT PaeR7I 1518 CTCGAG C^TCGAG 3748 CTCGAG PflFI 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2739 GACATGGTC PstI 222 CTGCAG CTGCA^G 3467 CTGCAG RcaI 2922 TCATGA T^CATGA 4593 TCATGA SacII 152 CCGCGG CCGC^GG 1596 CCGCGG SfoI 31 GGCGCC GGC^GCC 571 GGCGCC StuI 583 AGGCCT AGG^CCT 1453 AGGCCT Tth111I 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2739 GACATGGTC XbaI 2333 TCTAGA T^CTAGA 3689 TCTAGA XhoI 1518 CTCGAG C^TCGAG 3748 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AspEI AspHI AvaI AvaII AvrII BanI BanII BbvI BcnI BfaI BglI BlnI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI