##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AgeI Aor51HI AscI AseI AsnI
Asp700I AviII BamHI BbrPI BcgI
BclI BsiWI BsmBI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DrdI Eco47III EcoO65I EcoRI
EcoT22I FbaI FseI FspI HpaI
MfeI MluI MunI NaeI NgoMIV
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1539 GACGTC GACGT^C
Acc65I 1850 GGTACC G^GTACC
AccIII 1558 TCCGGA T^CCGGA
AclI 716 AACGTT AA^CGTT
AcsI 2312 AAATTT [GA]^AATT[CT]
AflII 4349 CTTAAG C^TTAAG
AflIII 3378 ACGTGT A^C[GA][CT]GT
ApaI 1212 GGGCCC GGGCC^C
ApoI 2312 AAATTT [GA]^AATT[CT]
Asp718I 1850 GGTACC G^GTACC
BalI 3549 TGGCCA TGG^CCA
BbvCI 1050 CCTCAGC CCTCAGC(-5/-2)
BfrI 4349 CTTAAG C^TTAAG
BlpI 4384 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4384 GCTCAGC GC^T[ACGT]AGC
BsaAI 813 TACGTG [CT]AC^GT[GA]
BsaBI 778 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1558 TCCGGA T^CCGGA
Bsp1407I 2588 TGTACA T^GTACA
BspDI 1710 ATCGAT AT^CGAT
BspEI 1558 TCCGGA T^CCGGA
BsrFI 601 GCCGGT [GA]^CCGG[CT]
BsrGI 2588 TGTACA T^GTACA
CelII 4384 GCTCAGC GC^T[ACGT]AGC
Cfr10I 601 GCCGGT [GA]^CCGG[CT]
ClaI 1710 ATCGAT AT^CGAT
EcoRV 3626 GATATC GAT^ATC
HgaI 4479 GACGC GACGC(5/10)
KpnI 1850 GGTACC GGTAC^C
MamI 778 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3549 TGGCCA TGG^CCA
MroI 1558 TCCGGA T^CCGGA
MscI 3549 TGGCCA TGG^CCA
NdeI 4108 CATATG CA^TATG
NheI 2353 GCTAGC G^CTAGC
NotI 48 GCGGCCGC GC^GGCCGC
Psp1406I 716 AACGTT AA^CGTT
SapI 4408 GCTCTTC GCTCTTC(1/4)
SexAI 397 ACCAGGT A^CC[AT]GGT
SspBI 2588 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 2779 GTCTAC GT^[AC][GT]AC
3758 GTAGAC
Alw44I 3308 GTGCAC G^TGCAC
3530 GTGCAC
AocI 3041 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
ApaLI 3308 GTGCAC G^TGCAC
3530 GTGCAC
AspI 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACATGGTC
BbeI 31 GGCGCC GGCGC^C
571 GGCGCC
BbsI 2873 GTCTTC GAAGAC(2/6)
4047 GTCTTC
BciVI 526 GTATCC GTATCC(6/5)
2439 GTATCC
BglII 1576 AGATCT A^GATCT
3198 AGATCT
BpuAI 2873 GTCTTC GAAGAC(2/6)
4047 GTCTTC
BsmI 591 GAATGC GAATGC(1/-1)
4078 GCATTC
BspHI 2922 TCATGA T^CATGA
4593 TCATGA
BsrDI 1799 CATTGC GCAATG(2/0)
4508 GCAATG
BssHII 549 GCGCGC G^CGCGC
551 GCGCGC
BssSI 1440 CACGAG CACGAG(-5/-1)
3389 CACGAG
BstAPI 598 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4399 GCATCGACTGC
BstXI 1168 CCAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1612 CCAGCTTTGTGG
Bsu36I 3041 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
Eco81I 3041 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
HindIII 282 AAGCTT A^AGCTT
666 AAGCTT
KasI 31 GGCGCC G^GCGCC
571 GGCGCC
KspI 152 CCGCGG CCGC^GG
1596 CCGCGG
MslI 1112 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4079 CATTCTTGTG
NarI 31 GGCGCC GG^CGCC
571 GGCGCC
NspI 434 GCATGT [GA]CATG^[CT]
2377 GCATGT
PaeR7I 1518 CTCGAG C^TCGAG
3748 CTCGAG
PflFI 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACATGGTC
PstI 222 CTGCAG CTGCA^G
3467 CTGCAG
RcaI 2922 TCATGA T^CATGA
4593 TCATGA
SacII 152 CCGCGG CCGC^GG
1596 CCGCGG
SfoI 31 GGCGCC GGC^GCC
571 GGCGCC
StuI 583 AGGCCT AGG^CCT
1453 AGGCCT
Tth111I 2546 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2739 GACATGGTC
XbaI 2333 TCTAGA T^CTAGA
3689 TCTAGA
XhoI 1518 CTCGAG C^TCGAG
3748 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII AvrII BanI BanII
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI