##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
Aor51HI ApaI AscI Asp700I AspI
AvaI AviII AvrII BalI BamHI
BbeI BcgI BciVI BlnI BsaHI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspLU11I BsrFI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI EaeI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI MluI MluNI
MscI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 683 GGTACC G^GTACC
AccIII 144 TCCGGA T^CCGGA
AhdI 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 683 GGTACC G^GTACC
AspEI 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 683 GGTACC G^G[CT][GA]CC
BbrPI 2979 CACGTG CAC^GTG
BbsI 3141 GTCTTC GAAGAC(2/6)
BbvCI 905 CCTCAGC CCTCAGC(-5/-2)
BclI 1106 TGATCA T^GATCA
BcnI 2682 CCCGG CC^[GC]GG
BglI 873 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 14 AGATCT A^GATCT
BpuAI 3141 GTCTTC GAAGAC(2/6)
BsaAI 2979 CACGTG [CT]AC^GT[GA]
BsaWI 144 TCCGGA [AT]^CCGG[AT]
BseAI 144 TCCGGA T^CCGGA
BsmI 1740 GCATTC GAATGC(1/-1)
Bsp1407I 1621 TGTACA T^GTACA
BspEI 144 TCCGGA T^CCGGA
BspMI 1702 GCAGGT ACCTGC(4/8)
BsrBI 2371 CCGCTC CCGCTC(-3/-3)
BsrGI 1621 TGTACA T^GTACA
BssHII 1153 GCGCGC G^CGCGC
DraIII 1567 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 492 GACCTTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2865 CCTGCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 818 CCTTGG C^C[AT][AT]GG
EcoT22I 3621 ATGCAT ATGCA^T
FbaI 1106 TGATCA T^GATCA
KpnI 683 GGTACC GGTAC^C
KspI 1311 CCGCGG CCGC^GG
MaeII 2980 ACGT A^CGT
MroI 144 TCCGGA T^CCGGA
NciI 2682 CCCGG CC^[GC]GG
NdeI 2160 CATATG CA^TATG
NheI 573 GCTAGC G^CTAGC
NsiI 3621 ATGCAT ATGCA^T
PflMI 4359 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2979 CACGTG CAC^GTG
PmlI 2979 CACGTG CAC^GTG
PvuII 1655 CAGCTG CAG^CTG
SacII 1311 CCGCGG CCGC^GG
SphI 1885 GCATGC GCATG^C
SspBI 1621 TGTACA T^GTACA
StyI 818 CCTTGG C^C[AT][AT]GG
Van91I 4359 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1250 GTCTAC GT^[AC][GT]AC
3726 GTAGAC
AccII 1154 CGCG CG^CG
1312 CGCG
AflII 168 CTTAAG C^TTAAG
1933 CTTAAG
AocI 3235 CCTTAGG CC^T[ACGT]AGG
3307 CCTCAGG
BanII 1232 GGGCTC G[GA]GC[CT]^C
1879 GAGCCC
BfrI 168 CTTAAG C^TTAAG
1933 CTTAAG
BsaBI 3627 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4041 GATGACAATC
BspHI 4298 TCATGA T^CATGA
4552 TCATGA
BstUI 1154 CGCG CG^CG
1312 CGCG
BstXI 1366 CCATCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1847 CCAGATACATGG
Bsu36I 3235 CCTTAGG CC^T[ACGT]AGG
3307 CCTCAGG
CfoI 1153 GCGC GCG^C
1155 GCGC
Eco81I 3235 CCTTAGG CC^T[ACGT]AGG
3307 CCTCAGG
HapII 145 CCGG C^CGG
2683 CCGG
HhaI 1153 GCGC GCG^C
1155 GCGC
HinP1I 1153 GCGC G^CGC
1155 GCGC
HpaII 145 CCGG C^CGG
2683 CCGG
MamI 3627 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4041 GATGACAATC
MspI 145 CCGG C^CGG
2683 CCGG
MvnI 1154 CGCG CG^CG
1312 CGCG
PstI 765 CTGCAG CTGCA^G
4086 CTGCAG
RcaI 4298 TCATGA T^CATGA
4552 TCATGA
XcmI 1768 CCATGTGCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4359 CCAAAGGTTGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AseI
AsnI AspHI AvaII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRI EcoRII FauI Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII