################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AclI AcyI AflIII AgeI Aor51HI ApaI AscI Asp700I AspI AvaI AviII AvrII BalI BamHI BbeI BcgI BciVI BlnI BsaHI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspLU11I BsrFI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI MluI MluNI MscI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 683 GGTACC G^GTACC AccIII 144 TCCGGA T^CCGGA AhdI 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 683 GGTACC G^GTACC AspEI 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 683 GGTACC G^G[CT][GA]CC BbrPI 2979 CACGTG CAC^GTG BbsI 3141 GTCTTC GAAGAC(2/6) BbvCI 905 CCTCAGC CCTCAGC(-5/-2) BclI 1106 TGATCA T^GATCA BcnI 2682 CCCGG CC^[GC]GG BglI 873 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 14 AGATCT A^GATCT BpuAI 3141 GTCTTC GAAGAC(2/6) BsaAI 2979 CACGTG [CT]AC^GT[GA] BsaWI 144 TCCGGA [AT]^CCGG[AT] BseAI 144 TCCGGA T^CCGGA BsmI 1740 GCATTC GAATGC(1/-1) Bsp1407I 1621 TGTACA T^GTACA BspEI 144 TCCGGA T^CCGGA BspMI 1702 GCAGGT ACCTGC(4/8) BsrBI 2371 CCGCTC CCGCTC(-3/-3) BsrGI 1621 TGTACA T^GTACA BssHII 1153 GCGCGC G^CGCGC DraIII 1567 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 492 GACCTTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 115 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2865 CCTGCATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 818 CCTTGG C^C[AT][AT]GG EcoT22I 3621 ATGCAT ATGCA^T FbaI 1106 TGATCA T^GATCA KpnI 683 GGTACC GGTAC^C KspI 1311 CCGCGG CCGC^GG MaeII 2980 ACGT A^CGT MroI 144 TCCGGA T^CCGGA NciI 2682 CCCGG CC^[GC]GG NdeI 2160 CATATG CA^TATG NheI 573 GCTAGC G^CTAGC NsiI 3621 ATGCAT ATGCA^T PflMI 4359 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2979 CACGTG CAC^GTG PmlI 2979 CACGTG CAC^GTG PvuII 1655 CAGCTG CAG^CTG SacII 1311 CCGCGG CCGC^GG SphI 1885 GCATGC GCATG^C SspBI 1621 TGTACA T^GTACA StyI 818 CCTTGG C^C[AT][AT]GG Van91I 4359 CCAAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1250 GTCTAC GT^[AC][GT]AC 3726 GTAGAC AccII 1154 CGCG CG^CG 1312 CGCG AflII 168 CTTAAG C^TTAAG 1933 CTTAAG AocI 3235 CCTTAGG CC^T[ACGT]AGG 3307 CCTCAGG BanII 1232 GGGCTC G[GA]GC[CT]^C 1879 GAGCCC BfrI 168 CTTAAG C^TTAAG 1933 CTTAAG BsaBI 3627 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4041 GATGACAATC BspHI 4298 TCATGA T^CATGA 4552 TCATGA BstUI 1154 CGCG CG^CG 1312 CGCG BstXI 1366 CCATCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1847 CCAGATACATGG Bsu36I 3235 CCTTAGG CC^T[ACGT]AGG 3307 CCTCAGG CfoI 1153 GCGC GCG^C 1155 GCGC Eco81I 3235 CCTTAGG CC^T[ACGT]AGG 3307 CCTCAGG HapII 145 CCGG C^CGG 2683 CCGG HhaI 1153 GCGC GCG^C 1155 GCGC HinP1I 1153 GCGC G^CGC 1155 GCGC HpaII 145 CCGG C^CGG 2683 CCGG MamI 3627 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4041 GATGACAATC MspI 145 CCGG C^CGG 2683 CCGG MvnI 1154 CGCG CG^CG 1312 CGCG PstI 765 CTGCAG CTGCA^G 4086 CTGCAG RcaI 4298 TCATGA T^CATGA 4552 TCATGA XcmI 1768 CCATGTGCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4359 CCAAAGGTTGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AseI AsnI AspHI AvaII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII