################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BbeI BcgI BfrI BglII BlnI BsiWI BspDI BspHI BsrDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII EcoRI HindIII HpaI KasI KpnI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Aor51HI 2766 AGCGCT AGC^GCT Asp700I 3372 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3157 TGCGCA TGC^GCA BclI 485 TGATCA T^GATCA BsaBI 3141 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 3177 CGGCCG CG[GA][CT]^CG BsmI 1934 GAATGC GAATGC(1/-1) DrdI 3154 GACTGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3177 CGGCCG C^GGCCG EclXI 3177 CGGCCG C^GGCCG Eco47III 2766 AGCGCT AGC^GCT Eco52I 3177 CGGCCG C^GGCCG EcoRV 1386 GATATC GAT^ATC EcoT22I 3755 ATGCAT ATGCA^T FbaI 485 TGATCA T^GATCA FspI 3157 TGCGCA TGC^GCA HincII 4422 GTCAAC GT[CT]^[GA]AC HindII 4422 GTCAAC GT[CT]^[GA]AC MamI 3141 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NsiI 3755 ATGCAT ATGCA^T PaeR7I 1497 CTCGAG C^TCGAG PflFI 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 262 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1782 GAGCTC GAGCT^C SexAI 2137 ACCTGGT A^CC[AT]GGT SfiI 4332 GGCCCGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspI 4537 AATATT AAT^ATT Tth111I 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1497 CTCGAG C^TCGAG XmnI 3372 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 181 GTCTAC GT^[AC][GT]AC 3964 GTAGAC AccIII 2353 TCCGGA T^CCGGA 3270 TCCGGA Alw44I 3769 GTGCAC G^TGCAC 3779 GTGCAC AocI 1731 CCTGAGG CC^T[ACGT]AGG 3880 CCTCAGG ApaLI 3769 GTGCAC G^TGCAC 3779 GTGCAC BamHI 3206 GGATCC G^GATCC 4357 GGATCC BbsI 3486 GAAGAC GAAGAC(2/6) 3163 GTCTTC BpuAI 3486 GAAGAC GAAGAC(2/6) 3163 GTCTTC BseAI 2353 TCCGGA T^CCGGA 3270 TCCGGA Bsp1407I 3736 TGTACA T^GTACA 4374 TGTACA BspEI 2353 TCCGGA T^CCGGA 3270 TCCGGA BsrGI 3736 TGTACA T^GTACA 4374 TGTACA BssHII 518 GCGCGC G^CGCGC 3387 GCGCGC BstEII 3886 GGTCACC G^GT[ACGT]ACC 4367 GGTTACC BstPI 3886 GGTCACC G^GT[ACGT]ACC 4367 GGTTACC Bsu36I 1731 CCTGAGG CC^T[ACGT]AGG 3880 CCTCAGG DraI 4412 TTTAAA TTT^AAA 4479 TTTAAA Eco81I 1731 CCTGAGG CC^T[ACGT]AGG 3880 CCTCAGG EcoO65I 3886 GGTCACC G^GT[ACGT]ACC 4367 GGTTACC FseI 541 GGCCGGCC GGCCGG^CC 776 GGCCGGCC MroI 2353 TCCGGA T^CCGGA 3270 TCCGGA SmaI 3285 CCCGGG CCC^GGG 4130 CCCGGG SphI 3672 GCATGC GCATG^C 3757 GCATGC SspBI 3736 TGTACA T^GTACA 4374 TGTACA StuI 1971 AGGCCT AGG^CCT 3010 AGGCCT XmaCI 3285 CCCGGG C^CCGGG 4130 CCCGGG XmaI 3285 CCCGGG C^CCGGG 4130 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII