##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BbeI BcgI BfrI BglII BlnI
BsiWI BspDI BspHI BsrDI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EcoRI HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 2766 AGCGCT AGC^GCT
Asp700I 3372 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3157 TGCGCA TGC^GCA
BclI 485 TGATCA T^GATCA
BsaBI 3141 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3177 CGGCCG CG[GA][CT]^CG
BsmI 1934 GAATGC GAATGC(1/-1)
DrdI 3154 GACTGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3177 CGGCCG C^GGCCG
EclXI 3177 CGGCCG C^GGCCG
Eco47III 2766 AGCGCT AGC^GCT
Eco52I 3177 CGGCCG C^GGCCG
EcoRV 1386 GATATC GAT^ATC
EcoT22I 3755 ATGCAT ATGCA^T
FbaI 485 TGATCA T^GATCA
FspI 3157 TGCGCA TGC^GCA
HincII 4422 GTCAAC GT[CT]^[GA]AC
HindII 4422 GTCAAC GT[CT]^[GA]AC
MamI 3141 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 3755 ATGCAT ATGCA^T
PaeR7I 1497 CTCGAG C^TCGAG
PflFI 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 262 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1782 GAGCTC GAGCT^C
SexAI 2137 ACCTGGT A^CC[AT]GGT
SfiI 4332 GGCCCGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 4537 AATATT AAT^ATT
Tth111I 3432 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1497 CTCGAG C^TCGAG
XmnI 3372 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 181 GTCTAC GT^[AC][GT]AC
3964 GTAGAC
AccIII 2353 TCCGGA T^CCGGA
3270 TCCGGA
Alw44I 3769 GTGCAC G^TGCAC
3779 GTGCAC
AocI 1731 CCTGAGG CC^T[ACGT]AGG
3880 CCTCAGG
ApaLI 3769 GTGCAC G^TGCAC
3779 GTGCAC
BamHI 3206 GGATCC G^GATCC
4357 GGATCC
BbsI 3486 GAAGAC GAAGAC(2/6)
3163 GTCTTC
BpuAI 3486 GAAGAC GAAGAC(2/6)
3163 GTCTTC
BseAI 2353 TCCGGA T^CCGGA
3270 TCCGGA
Bsp1407I 3736 TGTACA T^GTACA
4374 TGTACA
BspEI 2353 TCCGGA T^CCGGA
3270 TCCGGA
BsrGI 3736 TGTACA T^GTACA
4374 TGTACA
BssHII 518 GCGCGC G^CGCGC
3387 GCGCGC
BstEII 3886 GGTCACC G^GT[ACGT]ACC
4367 GGTTACC
BstPI 3886 GGTCACC G^GT[ACGT]ACC
4367 GGTTACC
Bsu36I 1731 CCTGAGG CC^T[ACGT]AGG
3880 CCTCAGG
DraI 4412 TTTAAA TTT^AAA
4479 TTTAAA
Eco81I 1731 CCTGAGG CC^T[ACGT]AGG
3880 CCTCAGG
EcoO65I 3886 GGTCACC G^GT[ACGT]ACC
4367 GGTTACC
FseI 541 GGCCGGCC GGCCGG^CC
776 GGCCGGCC
MroI 2353 TCCGGA T^CCGGA
3270 TCCGGA
SmaI 3285 CCCGGG CCC^GGG
4130 CCCGGG
SphI 3672 GCATGC GCATG^C
3757 GCATGC
SspBI 3736 TGTACA T^GTACA
4374 TGTACA
StuI 1971 AGGCCT AGG^CCT
3010 AGGCCT
XmaCI 3285 CCCGGG C^CCGGG
4130 CCCGGG
XmaI 3285 CCCGGG C^CCGGG
4130 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII