##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AcsI AhdI
ApoI AseI AsnI Asp700I Asp718I
AspEI AspI BbvCI BcgI BciVI
BclI BglII BlpI Bpu1102I BsaBI
BseAI BsiWI BsmBI BspDI BspEI
BssSI Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MamI MfeI MluI MroI
MunI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SapI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1469 GACGTC GACGT^C
AflII 3639 CTTAAG C^TTAAG
AgeI 2644 ACCGGT A^CCGGT
Alw44I 3361 GTGCAC G^TGCAC
ApaLI 3361 GTGCAC G^TGCAC
AscI 556 GGCGCGCC GG^CGCGCC
AviII 490 TGCGCA TGC^GCA
BamHI 2579 GGATCC G^GATCC
BbsI 3718 GAAGAC GAAGAC(2/6)
BfrI 3639 CTTAAG C^TTAAG
BpuAI 3718 GAAGAC GAAGAC(2/6)
BsaWI 2644 ACCGGT [AT]^CCGG[AT]
BsmI 2872 GAATGC GAATGC(1/-1)
Bsp1407I 3609 TGTACA T^GTACA
BsrDI 1758 GCAATG GCAATG(2/0)
BsrGI 3609 TGTACA T^GTACA
BstAPI 259 GCACGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 1039 GACGGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2682 GAAGAG CTCTTC(1/4)
FspI 490 TGCGCA TGC^GCA
HindIII 3615 AAGCTT A^AGCTT
Ksp632I 2682 GAAGAG CTCTTC(1/4)
KspI 829 CCGCGG CCGC^GG
NaeI 31 GCCGGC GCC^GGC
NdeI 1617 CATATG CA^TATG
NgoMIV 31 GCCGGC G^CCGGC
NheI 2505 GCTAGC G^CTAGC
PinAI 2644 ACCGGT A^CCGGT
SacII 829 CCGCGG CCGC^GG
SalI 932 GTCGAC G^TCGAC
SbfI 1862 CCTGCAGG CCTGCA^GG
SmaI 1632 CCCGGG CCC^GGG
SphI 846 GCATGC GCATG^C
Sse8387I 1862 CCTGCAGG CCTGCA^GG
SspBI 3609 TGTACA T^GTACA
XbaI 2868 TCTAGA T^CTAGA
XcmI 1299 CCATTGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1632 CCCGGG C^CCGGG
XmaI 1632 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 932 GTCGAC GT^[AC][GT]AC
1472 GTCTAC
AocI 3734 CCTTAGG CC^T[ACGT]AGG
3820 CCTGAGG
Aor51HI 485 AGCGCT AGC^GCT
1200 AGCGCT
AvrII 2514 CCTAGG C^CTAGG
2951 CCTAGG
BalI 426 TGGCCA TGG^CCA
1381 TGGCCA
BlnI 2514 CCTAGG C^CTAGG
2951 CCTAGG
BsgI 257 CTGCAC GTGCAG(16/14)
2033 GTGCAG
BspHI 1982 TCATGA T^CATGA
2613 TCATGA
BspLU11I 3545 ACATGT A^CATGT
3606 ACATGT
BsrBI 26 GAGCGG CCGCTC(-3/-3)
325 GAGCGG
BstEII 1971 GGTGACC G^GT[ACGT]ACC
2946 GGTGACC
BstPI 1971 GGTGACC G^GT[ACGT]ACC
2946 GGTGACC
BstXI 429 CCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
864 CCACCGGCCTGG
Bsu36I 3734 CCTTAGG CC^T[ACGT]AGG
3820 CCTGAGG
Eco47III 485 AGCGCT AGC^GCT
1200 AGCGCT
Eco81I 3734 CCTTAGG CC^T[ACGT]AGG
3820 CCTGAGG
EcoO65I 1971 GGTGACC G^GT[ACGT]ACC
2946 GGTGACC
HincII 99 GTCAAC GT[CT]^[GA]AC
932 GTCGAC
HindII 99 GTCAAC GT[CT]^[GA]AC
932 GTCGAC
MluNI 426 TGGCCA TGG^CCA
1381 TGGCCA
MscI 426 TGGCCA TGG^CCA
1381 TGGCCA
NcoI 1254 CCATGG C^CATGG
1326 CCATGG
NotI 150 GCGGCCGC GC^GGCCGC
185 GCGGCCGC
RcaI 1982 TCATGA T^CATGA
2613 TCATGA
ScaI 2251 AGTACT AGT^ACT
2549 AGTACT
StuI 459 AGGCCT AGG^CCT
1820 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII