##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI BamHI BanI BbeI
BbrPI BcgI BciVI BlpI Bpu1102I
BsaAI BsaBI BsiEI BsiWI BsmBI
BspMI BssHII CelII EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
HpaI KasI KpnI KspI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI SacII SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 87 GACGTC GACGT^C
AccII 125 CGCG CG^CG
AccIII 110 TCCGGA T^CCGGA
AflIII 3286 ACATGT A^C[GA][CT]GT
AseI 1691 ATTAAT AT^TAAT
AsnI 1691 ATTAAT AT^TAAT
AviII 964 TGCGCA TGC^GCA
AvrII 3997 CCTAGG C^CTAGG
BbvCI 1715 CCTCAGC CCTCAGC(-5/-2)
BglI 142 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1878 AGATCT A^GATCT
BlnI 3997 CCTAGG C^CTAGG
BsaWI 110 TCCGGA [AT]^CCGG[AT]
BseAI 110 TCCGGA T^CCGGA
BsgI 3145 GTGCAG GTGCAG(16/14)
BsmI 4209 GAATGC GAATGC(1/-1)
Bsp1407I 1802 TGTACA T^GTACA
BspEI 110 TCCGGA T^CCGGA
BspLU11I 3286 ACATGT A^CATGT
BsrBI 882 GAGCGG CCGCTC(-3/-3)
BsrGI 1802 TGTACA T^GTACA
BssSI 4341 CTCGTG CACGAG(-5/-1)
Bst1107I 1669 GTATAC GTA^TAC
BstBI 2396 TTCGAA TT^CGAA
BstUI 125 CGCG CG^CG
BstZ17I 1669 GTATAC GTA^TAC
CpoI 112 CGGACCG CG^G[AT]CCG
DraIII 237 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 587 GACCGAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1996 CCATGG C^C[GA][CT]GG
EcoNI 2555 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2648 GAATTC G^AATTC
EcoRV 774 GATATC GAT^ATC
FspI 964 TGCGCA TGC^GCA
HaeII 129 AGCGCC [GA]GCGC^[CT]
HgaI 1974 GCGTC GACGC(5/10)
MfeI 1730 CAATTG C^AATTG
MroI 110 TCCGGA T^CCGGA
MslI 3439 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1730 CAATTG C^AATTG
MvnI 125 CGCG CG^CG
NcoI 1996 CCATGG C^CATGG
NspV 2396 TTCGAA TT^CGAA
PshBI 1691 ATTAAT AT^TAAT
RsrII 112 CGGACCG CG^G[AT]CCG
SalI 1462 GTCGAC G^TCGAC
SapI 1005 GAAGAGC GCTCTTC(1/4)
SexAI 727 ACCTGGT A^CC[AT]GGT
SfuI 2396 TTCGAA TT^CGAA
SmaI 4062 CCCGGG CCC^GGG
SspBI 1802 TGTACA T^GTACA
SwaI 3870 ATTTAAAT ATTT^AAAT
XbaI 3976 TCTAGA T^CTAGA
XmaCI 4062 CCCGGG C^CCGGG
XmaI 4062 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 87 GACGTC G[GA]^CG[CT]C
1973 GGCGTC
AflII 2063 CTTAAG C^TTAAG
2351 CTTAAG
AocI 181 CCTGAGG CC^T[ACGT]AGG
2685 CCTGAGG
BalI 2227 TGGCCA TGG^CCA
3496 TGGCCA
BclI 1951 TGATCA T^GATCA
1960 TGATCA
BfrI 2063 CTTAAG C^TTAAG
2351 CTTAAG
BsaHI 87 GACGTC G[GA]^CG[CT]C
1973 GGCGTC
BsaI 2765 GAGACC GGTCTC(1/5)
3187 GAGACC
BspHI 1948 TCATGA T^CATGA
4362 TCATGA
BsrDI 489 GCAATG GCAATG(2/0)
1443 GCAATG
BsrFI 115 ACCGGC [GA]^CCGG[CT]
2670 ACCGGC
BstAPI 236 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
702 GCAAAATGTGC
BstEII 96 GGTGACC G^GT[ACGT]ACC
3432 GGTTACC
BstPI 96 GGTGACC G^GT[ACGT]ACC
3432 GGTTACC
BstXI 763 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3707 CCAGAGTGCTGG
Bsu36I 181 CCTGAGG CC^T[ACGT]AGG
2685 CCTGAGG
Cfr10I 115 ACCGGC [GA]^CCGG[CT]
2670 ACCGGC
Eco81I 181 CCTGAGG CC^T[ACGT]AGG
2685 CCTGAGG
EcoO65I 96 GGTGACC G^GT[ACGT]ACC
3432 GGTTACC
FbaI 1951 TGATCA T^GATCA
1960 TGATCA
Hin1I 87 GACGTC G[GA]^CG[CT]C
1973 GGCGTC
HincII 1462 GTCGAC GT[CT]^[GA]AC
4071 GTCAAC
HindII 1462 GTCGAC GT[CT]^[GA]AC
4071 GTCAAC
MluNI 2227 TGGCCA TGG^CCA
3496 TGGCCA
MscI 2227 TGGCCA TGG^CCA
3496 TGGCCA
NdeI 432 CATATG CA^TATG
648 CATATG
NspI 683 GCATGT [GA]CATG^[CT]
3286 ACATGT
PflMI 278 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCAGAAGGTGG
PleI 1275 GACTC GAGTC(4/5)
3054 GAGTC
RcaI 1948 TCATGA T^CATGA
4362 TCATGA
StuI 2976 AGGCCT AGG^CCT
3555 AGGCCT
Van91I 278 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1107 CCAGAAGGTGG
XcmI 199 CCAGGTGCATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4032 CCAGCTACAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I ClaI DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI