################################## # NO CUT ENZYMES ( 44 enzymes) # ################################## AatII Acc65I AclI AgeI AscI AseI AsnI Asp718I AvrII BamHI BbrPI BglII BlnI BsaBI BssHII BssSI CpoI HpaI KpnI KspI MamI MfeI MunI NcoI NdeI NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SacII SalI ScaI SfiI SgrAI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AhdI 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 67 AGCGCT AGC^GCT ApaI 34 GGGCCC GGGCC^C AspEI 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3387 TGCGCA TGC^GCA BalI 4272 TGGCCA TGG^CCA BciVI 4108 GGATAC GTATCC(6/5) BclI 289 TGATCA T^GATCA BsaAI 1245 TACGTG [CT]AC^GT[GA] BsiWI 3395 CGTACG C^GTACG BsmBI 2832 GAGACG CGTCTC(1/5) Bsp1407I 3284 TGTACA T^GTACA BspDI 864 ATCGAT AT^CGAT BsrBI 1498 CCGCTC CCGCTC(-3/-3) BsrGI 3284 TGTACA T^GTACA Bst1107I 378 GTATAC GTA^TAC BstAPI 448 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1516 TTCGAA TT^CGAA BstZ17I 378 GTATAC GTA^TAC ClaI 864 ATCGAT AT^CGAT DraIII 4879 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 5183 GACCACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 8 CGGCCG C^GGCCG Eam1105I 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 8 CGGCCG C^GGCCG Eco47III 67 AGCGCT AGC^GCT Eco52I 8 CGGCCG C^GGCCG EcoNI 200 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 289 TGATCA T^GATCA FseI 2098 GGCCGGCC GGCCGG^CC FspI 3387 TGCGCA TGC^GCA MluI 3392 ACGCGT A^CGCGT MluNI 4272 TGGCCA TGG^CCA MscI 4272 TGGCCA TGG^CCA NspV 1516 TTCGAA TT^CGAA PflMI 5362 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 460 CGATCG CGAT^CG SexAI 4595 ACCAGGT A^CC[AT]GGT SfuI 1516 TTCGAA TT^CGAA SphI 254 GCATGC GCATG^C SspBI 3284 TGTACA T^GTACA Van91I 5362 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2210 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AflII 4376 CTTAAG C^TTAAG 4404 CTTAAG Alw44I 1635 GTGCAC G^TGCAC 4479 GTGCAC AocI 1740 CCTCAGG CC^T[ACGT]AGG 2354 CCTGAGG ApaLI 1635 GTGCAC G^TGCAC 4479 GTGCAC AspI 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4547 GACAGGGTC BbeI 391 GGCGCC GGCGC^C 1354 GGCGCC BfrI 4376 CTTAAG C^TTAAG 4404 CTTAAG BlpI 1431 GCTCAGC GC^T[ACGT]AGC 5161 GCTCAGC Bpu1102I 1431 GCTCAGC GC^T[ACGT]AGC 5161 GCTCAGC BsmI 653 GAATGC GAATGC(1/-1) 947 GAATGC BspHI 766 TCATGA T^CATGA 4362 TCATGA BspLU11I 3526 ACATGT A^CATGT 5457 ACATGT BsrDI 1155 CATTGC GCAATG(2/0) 1193 CATTGC BstEII 56 GGTGACC G^GT[ACGT]ACC 4038 GGTAACC BstPI 56 GGTGACC G^GT[ACGT]ACC 4038 GGTAACC Bsu36I 1740 CCTCAGG CC^T[ACGT]AGG 2354 CCTGAGG CelII 1431 GCTCAGC GC^T[ACGT]AGC 5161 GCTCAGC DsaI 2920 CCACGG C^C[GA][CT]GG 4197 CCGTGG Eco81I 1740 CCTCAGG CC^T[ACGT]AGG 2354 CCTGAGG EcoO65I 56 GGTGACC G^GT[ACGT]ACC 4038 GGTAACC EcoRI 858 GAATTC G^AATTC 1900 GAATTC EcoRV 3848 GATATC GAT^ATC 5044 GATATC EcoT22I 949 ATGCAT ATGCA^T 4622 ATGCAT HincII 426 GTTGAC GT[CT]^[GA]AC 984 GTTGAC HindII 426 GTTGAC GT[CT]^[GA]AC 984 GTTGAC KasI 391 GGCGCC G^GCGCC 1354 GGCGCC NarI 391 GGCGCC GG^CGCC 1354 GGCGCC NsiI 949 ATGCAT ATGCA^T 4622 ATGCAT PaeR7I 1587 CTCGAG C^TCGAG 3587 CTCGAG PflFI 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4547 GACAGGGTC PshAI 3178 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5407 GACCTCTGTC RcaI 766 TCATGA T^CATGA 4362 TCATGA SacI 2086 GAGCTC GAGCT^C 2759 GAGCTC SapI 2486 GAAGAGC GCTCTTC(1/4) 2642 GAAGAGC SbfI 2515 CCTGCAGG CCTGCA^GG 2533 CCTGCAGG SfoI 391 GGCGCC GGC^GCC 1354 GGCGCC SmaI 26 CCCGGG CCC^GGG 3227 CCCGGG Sse8387I 2515 CCTGCAGG CCTGCA^GG 2533 CCTGCAGG SspI 411 AATATT AAT^ATT 4212 AATATT Tth111I 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 4547 GACAGGGTC XhoI 1587 CTCGAG C^TCGAG 3587 CTCGAG XmaCI 26 CCCGGG C^CCGGG 3227 CCCGGG XmaI 26 CCCGGG C^CCGGG 3227 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI