##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AvrII BamHI
BbrPI BglII BlnI BsaBI BssHII
BssSI CpoI HpaI KpnI KspI
MamI MfeI MunI NcoI NdeI
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RsrII SacII SalI ScaI SfiI
SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AhdI 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 67 AGCGCT AGC^GCT
ApaI 34 GGGCCC GGGCC^C
AspEI 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3387 TGCGCA TGC^GCA
BalI 4272 TGGCCA TGG^CCA
BciVI 4108 GGATAC GTATCC(6/5)
BclI 289 TGATCA T^GATCA
BsaAI 1245 TACGTG [CT]AC^GT[GA]
BsiWI 3395 CGTACG C^GTACG
BsmBI 2832 GAGACG CGTCTC(1/5)
Bsp1407I 3284 TGTACA T^GTACA
BspDI 864 ATCGAT AT^CGAT
BsrBI 1498 CCGCTC CCGCTC(-3/-3)
BsrGI 3284 TGTACA T^GTACA
Bst1107I 378 GTATAC GTA^TAC
BstAPI 448 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1516 TTCGAA TT^CGAA
BstZ17I 378 GTATAC GTA^TAC
ClaI 864 ATCGAT AT^CGAT
DraIII 4879 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5183 GACCACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 8 CGGCCG C^GGCCG
Eam1105I 666 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8 CGGCCG C^GGCCG
Eco47III 67 AGCGCT AGC^GCT
Eco52I 8 CGGCCG C^GGCCG
EcoNI 200 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 289 TGATCA T^GATCA
FseI 2098 GGCCGGCC GGCCGG^CC
FspI 3387 TGCGCA TGC^GCA
MluI 3392 ACGCGT A^CGCGT
MluNI 4272 TGGCCA TGG^CCA
MscI 4272 TGGCCA TGG^CCA
NspV 1516 TTCGAA TT^CGAA
PflMI 5362 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 460 CGATCG CGAT^CG
SexAI 4595 ACCAGGT A^CC[AT]GGT
SfuI 1516 TTCGAA TT^CGAA
SphI 254 GCATGC GCATG^C
SspBI 3284 TGTACA T^GTACA
Van91I 5362 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2210 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AflII 4376 CTTAAG C^TTAAG
4404 CTTAAG
Alw44I 1635 GTGCAC G^TGCAC
4479 GTGCAC
AocI 1740 CCTCAGG CC^T[ACGT]AGG
2354 CCTGAGG
ApaLI 1635 GTGCAC G^TGCAC
4479 GTGCAC
AspI 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4547 GACAGGGTC
BbeI 391 GGCGCC GGCGC^C
1354 GGCGCC
BfrI 4376 CTTAAG C^TTAAG
4404 CTTAAG
BlpI 1431 GCTCAGC GC^T[ACGT]AGC
5161 GCTCAGC
Bpu1102I 1431 GCTCAGC GC^T[ACGT]AGC
5161 GCTCAGC
BsmI 653 GAATGC GAATGC(1/-1)
947 GAATGC
BspHI 766 TCATGA T^CATGA
4362 TCATGA
BspLU11I 3526 ACATGT A^CATGT
5457 ACATGT
BsrDI 1155 CATTGC GCAATG(2/0)
1193 CATTGC
BstEII 56 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
BstPI 56 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
Bsu36I 1740 CCTCAGG CC^T[ACGT]AGG
2354 CCTGAGG
CelII 1431 GCTCAGC GC^T[ACGT]AGC
5161 GCTCAGC
DsaI 2920 CCACGG C^C[GA][CT]GG
4197 CCGTGG
Eco81I 1740 CCTCAGG CC^T[ACGT]AGG
2354 CCTGAGG
EcoO65I 56 GGTGACC G^GT[ACGT]ACC
4038 GGTAACC
EcoRI 858 GAATTC G^AATTC
1900 GAATTC
EcoRV 3848 GATATC GAT^ATC
5044 GATATC
EcoT22I 949 ATGCAT ATGCA^T
4622 ATGCAT
HincII 426 GTTGAC GT[CT]^[GA]AC
984 GTTGAC
HindII 426 GTTGAC GT[CT]^[GA]AC
984 GTTGAC
KasI 391 GGCGCC G^GCGCC
1354 GGCGCC
NarI 391 GGCGCC GG^CGCC
1354 GGCGCC
NsiI 949 ATGCAT ATGCA^T
4622 ATGCAT
PaeR7I 1587 CTCGAG C^TCGAG
3587 CTCGAG
PflFI 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4547 GACAGGGTC
PshAI 3178 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5407 GACCTCTGTC
RcaI 766 TCATGA T^CATGA
4362 TCATGA
SacI 2086 GAGCTC GAGCT^C
2759 GAGCTC
SapI 2486 GAAGAGC GCTCTTC(1/4)
2642 GAAGAGC
SbfI 2515 CCTGCAGG CCTGCA^GG
2533 CCTGCAGG
SfoI 391 GGCGCC GGC^GCC
1354 GGCGCC
SmaI 26 CCCGGG CCC^GGG
3227 CCCGGG
Sse8387I 2515 CCTGCAGG CCTGCA^GG
2533 CCTGCAGG
SspI 411 AATATT AAT^ATT
4212 AATATT
Tth111I 354 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4547 GACAGGGTC
XhoI 1587 CTCGAG C^TCGAG
3587 CTCGAG
XmaCI 26 CCCGGG C^CCGGG
3227 CCCGGG
XmaI 26 CCCGGG C^CCGGG
3227 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI