################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccII AccIII AclI AflII AflIII AgeI AhdI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrFI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcyI 2129 GACGCC G[GA]^CG[CT]C AocI 2402 CCTCAGG CC^T[ACGT]AGG Aor51HI 1352 AGCGCT AGC^GCT Asp700I 1129 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 2199 GGATCC G^GATCC BbvCI 2449 CCTCAGC CCTCAGC(-5/-2) BclI 4215 TGATCA T^GATCA BglI 2901 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4117 AGATCT A^GATCT BsaHI 2129 GACGCC G[GA]^CG[CT]C BsmI 15 GAATGC GAATGC(1/-1) BssSI 3596 CACGAG CACGAG(-5/-1) Bsu36I 2402 CCTCAGG CC^T[ACGT]AGG DraI 3990 TTTAAA TTT^AAA DraIII 3400 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1352 AGCGCT AGC^GCT Eco81I 2402 CCTCAGG CC^T[ACGT]AGG FbaI 4215 TGATCA T^GATCA Hin1I 2129 GACGCC G[GA]^CG[CT]C MfeI 3894 CAATTG C^AATTG MunI 3894 CAATTG C^AATTG NheI 3775 GCTAGC G^CTAGC SacI 4123 GAGCTC GAGCT^C SalI 2330 GTCGAC G^TCGAC ScaI 928 AGTACT AGT^ACT SfiI 2900 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 1443 ACTAGT A^CTAGT SspI 3833 AATATT AAT^ATT XbaI 2386 TCTAGA T^CTAGA XcmI 2003 CCAATTTAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1129 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 206 GGTACC G^GTACC 2733 GGTACC AccI 2330 GTCGAC GT^[AC][GT]AC 4334 GTAGAC ApaI 2908 GGGCCC GGGCC^C 3543 GGGCCC Asp718I 206 GGTACC G^GTACC 2733 GGTACC AvaI 3 CCCGGG C^[CT]CG[GA]G 4514 CCCGGG BalI 1404 TGGCCA TGG^CCA 3102 TGGCCA BlpI 2017 GCTCAGC GC^T[ACGT]AGC 2416 GCTGAGC Bpu1102I 2017 GCTCAGC GC^T[ACGT]AGC 2416 GCTGAGC BsmBI 2038 CGTCTC CGTCTC(1/5) 2127 GAGACG BsoBI 3 CCCGGG C^[CT]CG[GA]G 4514 CCCGGG Bsp1407I 1002 TGTACA T^GTACA 4246 TGTACA BsrBI 3844 CCGCTC CCGCTC(-3/-3) 55 GAGCGG BsrDI 1067 CATTGC GCAATG(2/0) 3922 CATTGC BsrGI 1002 TGTACA T^GTACA 4246 TGTACA BstEII 181 GGTGACC G^GT[ACGT]ACC 2863 GGTAACC BstPI 181 GGTGACC G^GT[ACGT]ACC 2863 GGTAACC BstXI 165 CCAAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2059 CCAGCAGTGTGG CelII 2017 GCTCAGC GC^T[ACGT]AGC 2416 GCTGAGC CfoI 1353 GCGC GCG^C 3026 GCGC EaeI 1404 TGGCCA [CT]^GGCC[GA] 3102 TGGCCA EcoNI 2476 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3193 CCTGAGACAGG EcoO65I 181 GGTGACC G^GT[ACGT]ACC 2863 GGTAACC EcoRI 371 GAATTC G^AATTC 2143 GAATTC FauI 3046 CCCGC CCCGC(4/6) 1799 GCGGG HaeII 1352 AGCGCT [GA]GCGC^[CT] 3025 AGCGCC HgaI 2129 GACGC GACGC(5/10) 118 GCGTC HhaI 1353 GCGC GCG^C 3026 GCGC HinP1I 1353 GCGC G^CGC 3026 GCGC HincII 2330 GTCGAC GT[CT]^[GA]AC 3787 GTCAAC HindII 2330 GTCGAC GT[CT]^[GA]AC 3787 GTCAAC HindIII 2381 AAGCTT A^AGCTT 2783 AAGCTT KpnI 206 GGTACC GGTAC^C 2733 GGTACC MluNI 1404 TGGCCA TGG^CCA 3102 TGGCCA MscI 1404 TGGCCA TGG^CCA 3102 TGGCCA MslI 1537 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4271 CATCCTTATG PflMI 1309 CCACGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4373 CCAATCCTTGG PstI 427 CTGCAG CTGCA^G 874 CTGCAG SmaI 3 CCCGGG CCC^GGG 4514 CCCGGG SspBI 1002 TGTACA T^GTACA 4246 TGTACA Van91I 1309 CCACGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4373 CCAATCCTTGG XmaCI 3 CCCGGG C^CCGGG 4514 CCCGGG XmaI 3 CCCGGG C^CCGGG 4514 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII