##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI FspI
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 2129 GACGCC G[GA]^CG[CT]C
AocI 2402 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1352 AGCGCT AGC^GCT
Asp700I 1129 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2199 GGATCC G^GATCC
BbvCI 2449 CCTCAGC CCTCAGC(-5/-2)
BclI 4215 TGATCA T^GATCA
BglI 2901 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4117 AGATCT A^GATCT
BsaHI 2129 GACGCC G[GA]^CG[CT]C
BsmI 15 GAATGC GAATGC(1/-1)
BssSI 3596 CACGAG CACGAG(-5/-1)
Bsu36I 2402 CCTCAGG CC^T[ACGT]AGG
DraI 3990 TTTAAA TTT^AAA
DraIII 3400 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1352 AGCGCT AGC^GCT
Eco81I 2402 CCTCAGG CC^T[ACGT]AGG
FbaI 4215 TGATCA T^GATCA
Hin1I 2129 GACGCC G[GA]^CG[CT]C
MfeI 3894 CAATTG C^AATTG
MunI 3894 CAATTG C^AATTG
NheI 3775 GCTAGC G^CTAGC
SacI 4123 GAGCTC GAGCT^C
SalI 2330 GTCGAC G^TCGAC
ScaI 928 AGTACT AGT^ACT
SfiI 2900 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1443 ACTAGT A^CTAGT
SspI 3833 AATATT AAT^ATT
XbaI 2386 TCTAGA T^CTAGA
XcmI 2003 CCAATTTAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1129 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 206 GGTACC G^GTACC
2733 GGTACC
AccI 2330 GTCGAC GT^[AC][GT]AC
4334 GTAGAC
ApaI 2908 GGGCCC GGGCC^C
3543 GGGCCC
Asp718I 206 GGTACC G^GTACC
2733 GGTACC
AvaI 3 CCCGGG C^[CT]CG[GA]G
4514 CCCGGG
BalI 1404 TGGCCA TGG^CCA
3102 TGGCCA
BlpI 2017 GCTCAGC GC^T[ACGT]AGC
2416 GCTGAGC
Bpu1102I 2017 GCTCAGC GC^T[ACGT]AGC
2416 GCTGAGC
BsmBI 2038 CGTCTC CGTCTC(1/5)
2127 GAGACG
BsoBI 3 CCCGGG C^[CT]CG[GA]G
4514 CCCGGG
Bsp1407I 1002 TGTACA T^GTACA
4246 TGTACA
BsrBI 3844 CCGCTC CCGCTC(-3/-3)
55 GAGCGG
BsrDI 1067 CATTGC GCAATG(2/0)
3922 CATTGC
BsrGI 1002 TGTACA T^GTACA
4246 TGTACA
BstEII 181 GGTGACC G^GT[ACGT]ACC
2863 GGTAACC
BstPI 181 GGTGACC G^GT[ACGT]ACC
2863 GGTAACC
BstXI 165 CCAAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2059 CCAGCAGTGTGG
CelII 2017 GCTCAGC GC^T[ACGT]AGC
2416 GCTGAGC
CfoI 1353 GCGC GCG^C
3026 GCGC
EaeI 1404 TGGCCA [CT]^GGCC[GA]
3102 TGGCCA
EcoNI 2476 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3193 CCTGAGACAGG
EcoO65I 181 GGTGACC G^GT[ACGT]ACC
2863 GGTAACC
EcoRI 371 GAATTC G^AATTC
2143 GAATTC
FauI 3046 CCCGC CCCGC(4/6)
1799 GCGGG
HaeII 1352 AGCGCT [GA]GCGC^[CT]
3025 AGCGCC
HgaI 2129 GACGC GACGC(5/10)
118 GCGTC
HhaI 1353 GCGC GCG^C
3026 GCGC
HinP1I 1353 GCGC G^CGC
3026 GCGC
HincII 2330 GTCGAC GT[CT]^[GA]AC
3787 GTCAAC
HindII 2330 GTCGAC GT[CT]^[GA]AC
3787 GTCAAC
HindIII 2381 AAGCTT A^AGCTT
2783 AAGCTT
KpnI 206 GGTACC GGTAC^C
2733 GGTACC
MluNI 1404 TGGCCA TGG^CCA
3102 TGGCCA
MscI 1404 TGGCCA TGG^CCA
3102 TGGCCA
MslI 1537 CACCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4271 CATCCTTATG
PflMI 1309 CCACGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4373 CCAATCCTTGG
PstI 427 CTGCAG CTGCA^G
874 CTGCAG
SmaI 3 CCCGGG CCC^GGG
4514 CCCGGG
SspBI 1002 TGTACA T^GTACA
4246 TGTACA
Van91I 1309 CCACGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4373 CCAATCCTTGG
XmaCI 3 CCCGGG C^CCGGG
4514 CCCGGG
XmaI 3 CCCGGG C^CCGGG
4514 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII