##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII BbeI BciVI BclI BfrI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoT22I FbaI FseI FspI
HpaI KasI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 3391 GGTACC G^GTACC
AccI 3035 GTAGAC GT^[AC][GT]AC
AclI 900 AACGTT AA^CGTT
AcyI 843 GGCGTC G[GA]^CG[CT]C
AlwNI 331 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 3391 GGTACC G^GTACC
AvrII 1955 CCTAGG C^CTAGG
BamHI 1170 GGATCC G^GATCC
BbrPI 3008 CACGTG CAC^GTG
BglI 1009 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1288 AGATCT A^GATCT
BlnI 1955 CCTAGG C^CTAGG
BsaAI 3008 CACGTG [CT]AC^GT[GA]
BsaBI 1641 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 843 GGCGTC G[GA]^CG[CT]C
BsiEI 168 CGGTCG CG[GA][CT]^CG
BsmI 3096 GAATGC GAATGC(1/-1)
BspHI 619 TCATGA T^CATGA
BspLU11I 1976 ACATGT A^CATGT
BsrDI 713 CATTGC GCAATG(2/0)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BstAPI 911 GCACCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 294 GGTGACC G^GT[ACGT]ACC
BstPI 294 GGTGACC G^GT[ACGT]ACC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
DraII 3657 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 3657 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 294 GGTGACC G^GT[ACGT]ACC
EcoRV 2355 GATATC GAT^ATC
Hin1I 843 GGCGTC G[GA]^CG[CT]C
HincII 3465 GTTGAC GT[CT]^[GA]AC
HindII 3465 GTTGAC GT[CT]^[GA]AC
HindIII 81 AAGCTT A^AGCTT
KpnI 3391 GGTACC GGTAC^C
MamI 1641 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 112 GCCGGC GCC^GGC
NcoI 1478 CCATGG C^CATGG
NgoMIV 112 GCCGGC G^CCGGC
NheI 2592 GCTAGC G^CTAGC
PaeR7I 359 CTCGAG C^TCGAG
PflMI 4196 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3008 CACGTG CAC^GTG
PmlI 3008 CACGTG CAC^GTG
PpuMI 3657 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 900 AACGTT AA^CGTT
RcaI 619 TCATGA T^CATGA
SapI 1458 GCTCTTC GCTCTTC(1/4)
SmaI 4064 CCCGGG CCC^GGG
SpeI 3327 ACTAGT A^CTAGT
Van91I 4196 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3960 CCAGTGCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 359 CTCGAG C^TCGAG
XmaCI 4064 CCCGGG C^CCGGG
XmaI 4064 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 1976 ACATGT A^C[GA][CT]GT
3007 ACACGT
Asp700I 1240 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3489 GAACGCTTTC
BcgI 361 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3843 GCAGAAGACTCG
BsaI 1633 GAGACC GGTCTC(1/5)
1421 GGTCTC
BsmBI 182 CGTCTC CGTCTC(1/5)
1327 CGTCTC
BssHII 211 GCGCGC G^CGCGC
283 GCGCGC
BssSI 117 CTCGTG CACGAG(-5/-1)
3851 CTCGTG
BstXI 1934 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3662 CCAGCAGGTTGG
DraIII 663 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
912 CACCCCGTG
HaeII 333 GGCGCT [GA]GCGC^[CT]
363 AGCGCC
KspI 98 CCGCGG CCGC^GG
152 CCGCGG
MaeII 901 ACGT A^CGT
3009 ACGT
PshAI 2625 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3849 GACTCGTGTC
SacII 98 CCGCGG CCGC^GG
152 CCGCGG
SspI 2784 AATATT AAT^ATT
4541 AATATT
XbaI 4007 TCTAGA T^CTAGA
4508 TCTAGA
XmnI 1240 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3489 GAACGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoNI EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII