################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccIII AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII BbeI BciVI BclI BfrI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrBI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FbaI FseI FspI HpaI KasI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 3391 GGTACC G^GTACC AccI 3035 GTAGAC GT^[AC][GT]AC AclI 900 AACGTT AA^CGTT AcyI 843 GGCGTC G[GA]^CG[CT]C AlwNI 331 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 3391 GGTACC G^GTACC AvrII 1955 CCTAGG C^CTAGG BamHI 1170 GGATCC G^GATCC BbrPI 3008 CACGTG CAC^GTG BglI 1009 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1288 AGATCT A^GATCT BlnI 1955 CCTAGG C^CTAGG BsaAI 3008 CACGTG [CT]AC^GT[GA] BsaBI 1641 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 843 GGCGTC G[GA]^CG[CT]C BsiEI 168 CGGTCG CG[GA][CT]^CG BsmI 3096 GAATGC GAATGC(1/-1) BspHI 619 TCATGA T^CATGA BspLU11I 1976 ACATGT A^CATGT BsrDI 713 CATTGC GCAATG(2/0) BsrFI 112 GCCGGC [GA]^CCGG[CT] BstAPI 911 GCACCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 294 GGTGACC G^GT[ACGT]ACC BstPI 294 GGTGACC G^GT[ACGT]ACC Cfr10I 112 GCCGGC [GA]^CCGG[CT] DraII 3657 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 3657 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 294 GGTGACC G^GT[ACGT]ACC EcoRV 2355 GATATC GAT^ATC Hin1I 843 GGCGTC G[GA]^CG[CT]C HincII 3465 GTTGAC GT[CT]^[GA]AC HindII 3465 GTTGAC GT[CT]^[GA]AC HindIII 81 AAGCTT A^AGCTT KpnI 3391 GGTACC GGTAC^C MamI 1641 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 112 GCCGGC GCC^GGC NcoI 1478 CCATGG C^CATGG NgoMIV 112 GCCGGC G^CCGGC NheI 2592 GCTAGC G^CTAGC PaeR7I 359 CTCGAG C^TCGAG PflMI 4196 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3008 CACGTG CAC^GTG PmlI 3008 CACGTG CAC^GTG PpuMI 3657 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 900 AACGTT AA^CGTT RcaI 619 TCATGA T^CATGA SapI 1458 GCTCTTC GCTCTTC(1/4) SmaI 4064 CCCGGG CCC^GGG SpeI 3327 ACTAGT A^CTAGT Van91I 4196 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3960 CCAGTGCCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 359 CTCGAG C^TCGAG XmaCI 4064 CCCGGG C^CCGGG XmaI 4064 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 1976 ACATGT A^C[GA][CT]GT 3007 ACACGT Asp700I 1240 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3489 GAACGCTTTC BcgI 361 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3843 GCAGAAGACTCG BsaI 1633 GAGACC GGTCTC(1/5) 1421 GGTCTC BsmBI 182 CGTCTC CGTCTC(1/5) 1327 CGTCTC BssHII 211 GCGCGC G^CGCGC 283 GCGCGC BssSI 117 CTCGTG CACGAG(-5/-1) 3851 CTCGTG BstXI 1934 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3662 CCAGCAGGTTGG DraIII 663 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 912 CACCCCGTG HaeII 333 GGCGCT [GA]GCGC^[CT] 363 AGCGCC KspI 98 CCGCGG CCGC^GG 152 CCGCGG MaeII 901 ACGT A^CGT 3009 ACGT PshAI 2625 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3849 GACTCGTGTC SacII 98 CCGCGG CCGC^GG 152 CCGCGG SspI 2784 AATATT AAT^ATT 4541 AATATT XbaI 4007 TCTAGA T^CTAGA 4508 TCTAGA XmnI 1240 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3489 GAACGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoNI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII