##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccII AclI AflIII AgeI
AscI AseI AsnI Asp718I BamHI
BbrPI BciVI BclI BsaAI BsiWI
BspDI BspLU11I BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
EcoRI FbaI FseI HpaI KpnI
KspI MfeI MluI MunI MvnI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 4044 GACGTC GACGT^C
AflII 1384 CTTAAG C^TTAAG
Alw44I 2219 GTGCAC G^TGCAC
ApaLI 2219 GTGCAC G^TGCAC
AspI 1294 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 885 TGCGCA TGC^GCA
AvrII 4514 CCTAGG C^CTAGG
BbeI 2620 GGCGCC GGCGC^C
BbsI 963 GAAGAC GAAGAC(2/6)
BfrI 1384 CTTAAG C^TTAAG
BglII 1937 AGATCT A^GATCT
BlnI 4514 CCTAGG C^CTAGG
BpuAI 963 GAAGAC GAAGAC(2/6)
BsaBI 2451 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 159 GAATGC GAATGC(1/-1)
BspHI 1404 TCATGA T^CATGA
BssSI 1195 CACGAG CACGAG(-5/-1)
BstXI 1800 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4750 TTTAAA TTT^AAA
DraIII 3224 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 484 GACCTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1626 GATATC GAT^ATC
EcoT22I 4740 ATGCAT ATGCA^T
FspI 885 TGCGCA TGC^GCA
KasI 2620 GGCGCC G^GCGCC
MamI 2451 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 6 GCCGGC GCC^GGC
NarI 2620 GGCGCC GG^CGCC
NgoMIV 6 GCCGGC G^CCGGC
NsiI 4740 ATGCAT ATGCA^T
PaeR7I 3059 CTCGAG C^TCGAG
PflFI 1294 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2824 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1404 TCATGA T^CATGA
SacI 1779 GAGCTC GAGCT^C
SapI 4005 GAAGAGC GCTCTTC(1/4)
SbfI 2046 CCTGCAGG CCTGCA^GG
SfoI 2620 GGCGCC GGC^GCC
SpeI 3037 ACTAGT A^CTAGT
Sse8387I 2046 CCTGCAGG CCTGCA^GG
SspI 4718 AATATT AAT^ATT
Tth111I 1294 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2824 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3059 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 4047 GTCTAC GT^[AC][GT]AC
4827 GTCTAC
AccIII 1265 TCCGGA T^CCGGA
2712 TCCGGA
AhdI 411 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2433 GACCAGGGGTC
Aor51HI 1380 AGCGCT AGC^GCT
4652 AGCGCT
AspEI 411 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2433 GACCAGGGGTC
BcgI 1341 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4076 GCACTATGTTCG
BsaWI 1265 TCCGGA [AT]^CCGG[AT]
2712 TCCGGA
BseAI 1265 TCCGGA T^CCGGA
2712 TCCGGA
Bsp1407I 4562 TGTACA T^GTACA
4711 TGTACA
BspEI 1265 TCCGGA T^CCGGA
2712 TCCGGA
BsrGI 4562 TGTACA T^GTACA
4711 TGTACA
DsaI 1800 CCATGG C^C[GA][CT]GG
2552 CCATGG
Eam1105I 411 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2433 GACCAGGGGTC
Eco47III 1380 AGCGCT AGC^GCT
4652 AGCGCT
HincII 841 GTCAAC GT[CT]^[GA]AC
949 GTCAAC
HindII 841 GTCAAC GT[CT]^[GA]AC
949 GTCAAC
HindIII 1614 AAGCTT A^AGCTT
2737 AAGCTT
MroI 1265 TCCGGA T^CCGGA
2712 TCCGGA
NcoI 1800 CCATGG C^CATGG
2552 CCATGG
PstI 2047 CTGCAG CTGCA^G
2308 CTGCAG
SspBI 4562 TGTACA T^GTACA
4711 TGTACA
TfiI 665 GATTC G^A[AT]TC
4235 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI