##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BbrPI BciVI BglI BlnI
BseAI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI BstEII BstPI ClaI
CpoI DrdI EagI EclXI Eco52I
EcoO65I FseI FspI HindIII KasI
KpnI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 223 GACGTC GACGT^C
AflII 959 CTTAAG C^TTAAG
Alw44I 3395 GTGCAC G^TGCAC
ApaLI 3395 GTGCAC G^TGCAC
BbvCI 547 GCTGAGG CCTCAGC(-5/-2)
BcgI 2631 GCACCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3207 TGATCA T^GATCA
BfrI 959 CTTAAG C^TTAAG
BlpI 580 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 580 GCTCAGC GC^T[ACGT]AGC
BsaBI 2244 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3830 CGGTCG CG[GA][CT]^CG
BsiWI 3762 CGTACG C^GTACG
BspLU11I 183 ACATGT A^CATGT
BsrFI 1566 ACCGGC [GA]^CCGG[CT]
Bst1107I 3197 GTATAC GTA^TAC
BstBI 948 TTCGAA TT^CGAA
BstZ17I 3197 GTATAC GTA^TAC
CelII 580 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1566 ACCGGC [GA]^CCGG[CT]
DraI 904 TTTAAA TTT^AAA
DraIII 3057 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2955 CCGTGG C^C[GA][CT]GG
EcoNI 4014 CCTTAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4106 GAATTC G^AATTC
EcoRV 511 GATATC GAT^ATC
FbaI 3207 TGATCA T^GATCA
HpaI 3755 GTTAAC GTT^AAC
MamI 2244 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 948 TTCGAA TT^CGAA
SalI 179 GTCGAC G^TCGAC
SfuI 948 TTCGAA TT^CGAA
SmaI 45 CCCGGG CCC^GGG
SspI 295 AATATT AAT^ATT
StuI 3247 AGGCCT AGG^CCT
XbaI 1955 TCTAGA T^CTAGA
XcmI 209 CCATCAAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 72 CGCG CG^CG
4173 CGCG
AocI 465 CCTCAGG CC^T[ACGT]AGG
4014 CCTTAGG
Aor51HI 577 AGCGCT AGC^GCT
775 AGCGCT
ApaI 740 GGGCCC GGGCC^C
4194 GGGCCC
Asp700I 835 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2390 GAAGATGTTC
AspI 2614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTCAGTC
BalI 2367 TGGCCA TGG^CCA
3184 TGGCCA
BamHI 2183 GGATCC G^GATCC
3590 GGATCC
BglII 2722 AGATCT A^GATCT
2922 AGATCT
BsgI 2289 GTGCAG GTGCAG(16/14)
4140 GTGCAG
BsmI 543 GAATGC GAATGC(1/-1)
2706 GCATTC
BspMI 4168 ACCTGC ACCTGC(4/8)
943 GCAGGT
BstUI 72 CGCG CG^CG
4173 CGCG
Bsu36I 465 CCTCAGG CC^T[ACGT]AGG
4014 CCTTAGG
Eco47III 577 AGCGCT AGC^GCT
775 AGCGCT
Eco81I 465 CCTCAGG CC^T[ACGT]AGG
4014 CCTTAGG
EcoT22I 761 ATGCAT ATGCA^T
3468 ATGCAT
HaeII 577 AGCGCT [GA]GCGC^[CT]
775 AGCGCT
MluNI 2367 TGGCCA TGG^CCA
3184 TGGCCA
MscI 2367 TGGCCA TGG^CCA
3184 TGGCCA
MvnI 72 CGCG CG^CG
4173 CGCG
NsiI 761 ATGCAT ATGCA^T
3468 ATGCAT
PaeR7I 1367 CTCGAG C^TCGAG
1804 CTCGAG
PflFI 2614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTCAGTC
PpuMI 110 GGGACCC [GA]G^G[AT]CC[CT]
1866 AGGACCC
PshAI 1868 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4150 GACAGCTGTC
Tth111I 2614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2842 GACTCAGTC
XhoI 1367 CTCGAG C^TCGAG
1804 CTCGAG
XmnI 835 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2390 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII