##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AclI AflII AflIII AgeI Alw44I
ApaLI AscI AseI AsnI AviII
BamHI BbrPI BcgI BfrI BsiEI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI BstEII BstPI
ClaI CpoI DraI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI MfeI MluI MunI
NdeI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 4045 AAATTT [GA]^AATT[CT]
ApoI 4045 AAATTT [GA]^AATT[CT]
Asp700I 1285 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 64 GGCGCC GGCGC^C
BclI 1704 TGATCA T^GATCA
BsrDI 441 GCAATG GCAATG(2/0)
Bst1107I 1268 GTATAC GTA^TAC
BstBI 2949 TTCGAA TT^CGAA
BstZ17I 1268 GTATAC GTA^TAC
FbaI 1704 TGATCA T^GATCA
HindIII 3040 AAGCTT A^AGCTT
HpaI 2447 GTTAAC GTT^AAC
KasI 64 GGCGCC G^GCGCC
MseI 2448 TTAA T^TAA
NarI 64 GGCGCC GG^CGCC
NcoI 1254 CCATGG C^CATGG
NheI 1504 GCTAGC G^CTAGC
NspV 2949 TTCGAA TT^CGAA
PflMI 2767 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1566 ACCAGGT A^CC[AT]GGT
SfoI 64 GGCGCC GGC^GCC
SfuI 2949 TTCGAA TT^CGAA
SgrAI 60 CACCGGCG C[GA]^CCGG[CT]G
SphI 1912 GCATGC GCATG^C
Tru9I 2448 TTAA T^TAA
Van91I 2767 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1285 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 563 GACGTC GACGT^C
3686 GACGTC
Acc65I 1798 GGTACC G^GTACC
2762 GGTACC
AhdI 1487 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2407 GACCCTCAGTC
Aor51HI 221 AGCGCT AGC^GCT
1554 AGCGCT
Asp718I 1798 GGTACC G^GTACC
2762 GGTACC
AspEI 1487 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2407 GACCCTCAGTC
AvrII 664 CCTAGG C^CTAGG
1003 CCTAGG
BciVI 1053 GGATAC GTATCC(6/5)
4098 GGATAC
BglII 2547 AGATCT A^GATCT
3735 AGATCT
BlnI 664 CCTAGG C^CTAGG
1003 CCTAGG
BlpI 1102 GCTGAGC GC^T[ACGT]AGC
2959 GCTGAGC
Bpu1102I 1102 GCTGAGC GC^T[ACGT]AGC
2959 GCTGAGC
BsaAI 1034 TACGTG [CT]AC^GT[GA]
1817 TACGTG
BsaBI 626 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3175 GATCAACATC
BsmBI 1523 GAGACG CGTCTC(1/5)
1676 GAGACG
BspHI 780 TCATGA T^CATGA
3484 TCATGA
BstAPI 1996 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2800 GCAGCCTGTGC
CelII 1102 GCTGAGC GC^T[ACGT]AGC
2959 GCTGAGC
DraIII 645 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3080 CACATGGTG
Eam1105I 1487 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2407 GACCCTCAGTC
Eco47III 221 AGCGCT AGC^GCT
1554 AGCGCT
KpnI 1798 GGTACC GGTAC^C
2762 GGTACC
KspI 18 CCGCGG CCGC^GG
69 CCGCGG
MamI 626 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3175 GATCAACATC
RcaI 780 TCATGA T^CATGA
3484 TCATGA
SacII 18 CCGCGG CCGC^GG
69 CCGCGG
SapI 499 GAAGAGC GCTCTTC(1/4)
3126 GCTCTTC
Tsp509I 514 AATT ^AATT
4046 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI