################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccI AccIII AclI AcyI ApaI AscI AseI AsnI Asp700I AviII AvrII BbeI BciVI BlnI BsaHI BseAI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EcoRV EcoT22I FseI FspI Hin1I KasI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SspI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 5866 GGTACC G^GTACC AflII 2524 CTTAAG C^TTAAG AgeI 1412 ACCGGT A^CCGGT Aor51HI 978 AGCGCT AGC^GCT Asp718I 5866 GGTACC G^GTACC BamHI 5643 GGATCC G^GATCC BbrPI 5159 CACGTG CAC^GTG BcgI 5158 GCACGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4255 TGATCA T^GATCA BcnI 2322 CCCGG CC^[GC]GG BfrI 2524 CTTAAG C^TTAAG BsaBI 1738 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1412 ACCGGT [AT]^CCGG[AT] BsiEI 4635 CGGCCG CG[GA][CT]^CG BsiWI 3845 CGTACG C^GTACG Bsp1407I 4600 TGTACA T^GTACA BsrFI 1412 ACCGGT [GA]^CCGG[CT] BsrGI 4600 TGTACA T^GTACA BstEII 5750 GGTGACC G^GT[ACGT]ACC BstPI 5750 GGTGACC G^GT[ACGT]ACC Cfr10I 1412 ACCGGT [GA]^CCGG[CT] DraIII 2561 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 4635 CGGCCG C^GGCCG EclXI 4635 CGGCCG C^GGCCG Eco47III 978 AGCGCT AGC^GCT Eco52I 4635 CGGCCG C^GGCCG EcoO65I 5750 GGTGACC G^GT[ACGT]ACC FbaI 4255 TGATCA T^GATCA HaeII 978 AGCGCT [GA]GCGC^[CT] HgaI 2410 GCGTC GACGC(5/10) HincII 4322 GTTAAC GT[CT]^[GA]AC HindII 4322 GTTAAC GT[CT]^[GA]AC HpaI 4322 GTTAAC GTT^AAC KpnI 5866 GGTACC GGTAC^C MamI 1738 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 2322 CCCGG CC^[GC]GG PaeR7I 379 CTCGAG C^TCGAG PinAI 1412 ACCGGT A^CCGGT PmaCI 5159 CACGTG CAC^GTG PmlI 5159 CACGTG CAC^GTG PshAI 386 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 5857 AGTACT AGT^ACT SnaBI 3843 TACGTA TAC^GTA SphI 2170 GCATGC GCATG^C SspBI 4600 TGTACA T^GTACA XbaI 2936 TCTAGA T^CTAGA XhoI 379 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 3849 CGCG CG^CG 3851 CGCG AhdI 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2550 GACATGGAGTC AspEI 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2550 GACATGGAGTC AspI 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5775 GACAGAGTC BbvCI 3961 GCTGAGG CCTCAGC(-5/-2) 5826 GCTGAGG BlpI 1113 GCTCAGC GC^T[ACGT]AGC 1520 GCTTAGC Bpu1102I 1113 GCTCAGC GC^T[ACGT]AGC 1520 GCTTAGC BsmBI 225 CGTCTC CGTCTC(1/5) 5245 GAGACG BstUI 3849 CGCG CG^CG 3851 CGCG CelII 1113 GCTCAGC GC^T[ACGT]AGC 1520 GCTTAGC Eam1105I 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2550 GACATGGAGTC HindIII 38 AAGCTT A^AGCTT 3103 AAGCTT MvnI 3849 CGCG CG^CG 3851 CGCG NheI 1035 GCTAGC G^CTAGC 2174 GCTAGC PflFI 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5775 GACAGAGTC PpuMI 2208 GGGACCT [GA]G^G[AT]CC[CT] 3237 GGGACCT SapI 480 GAAGAGC GCTCTTC(1/4) 560 GCTCTTC SbfI 5452 CCTGCAGG CCTGCA^GG 5755 CCTGCAGG Sse8387I 5452 CCTGCAGG CCTGCA^GG 5755 CCTGCAGG Tth111I 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC 5775 GACAGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII