##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
ApaI AscI AseI AsnI Asp700I
AviII AvrII BbeI BciVI BlnI
BsaHI BseAI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoRV EcoT22I
FseI FspI Hin1I KasI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SspI SwaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 5866 GGTACC G^GTACC
AflII 2524 CTTAAG C^TTAAG
AgeI 1412 ACCGGT A^CCGGT
Aor51HI 978 AGCGCT AGC^GCT
Asp718I 5866 GGTACC G^GTACC
BamHI 5643 GGATCC G^GATCC
BbrPI 5159 CACGTG CAC^GTG
BcgI 5158 GCACGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4255 TGATCA T^GATCA
BcnI 2322 CCCGG CC^[GC]GG
BfrI 2524 CTTAAG C^TTAAG
BsaBI 1738 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1412 ACCGGT [AT]^CCGG[AT]
BsiEI 4635 CGGCCG CG[GA][CT]^CG
BsiWI 3845 CGTACG C^GTACG
Bsp1407I 4600 TGTACA T^GTACA
BsrFI 1412 ACCGGT [GA]^CCGG[CT]
BsrGI 4600 TGTACA T^GTACA
BstEII 5750 GGTGACC G^GT[ACGT]ACC
BstPI 5750 GGTGACC G^GT[ACGT]ACC
Cfr10I 1412 ACCGGT [GA]^CCGG[CT]
DraIII 2561 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4635 CGGCCG C^GGCCG
EclXI 4635 CGGCCG C^GGCCG
Eco47III 978 AGCGCT AGC^GCT
Eco52I 4635 CGGCCG C^GGCCG
EcoO65I 5750 GGTGACC G^GT[ACGT]ACC
FbaI 4255 TGATCA T^GATCA
HaeII 978 AGCGCT [GA]GCGC^[CT]
HgaI 2410 GCGTC GACGC(5/10)
HincII 4322 GTTAAC GT[CT]^[GA]AC
HindII 4322 GTTAAC GT[CT]^[GA]AC
HpaI 4322 GTTAAC GTT^AAC
KpnI 5866 GGTACC GGTAC^C
MamI 1738 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 2322 CCCGG CC^[GC]GG
PaeR7I 379 CTCGAG C^TCGAG
PinAI 1412 ACCGGT A^CCGGT
PmaCI 5159 CACGTG CAC^GTG
PmlI 5159 CACGTG CAC^GTG
PshAI 386 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 5857 AGTACT AGT^ACT
SnaBI 3843 TACGTA TAC^GTA
SphI 2170 GCATGC GCATG^C
SspBI 4600 TGTACA T^GTACA
XbaI 2936 TCTAGA T^CTAGA
XhoI 379 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 3849 CGCG CG^CG
3851 CGCG
AhdI 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2550 GACATGGAGTC
AspEI 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2550 GACATGGAGTC
AspI 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5775 GACAGAGTC
BbvCI 3961 GCTGAGG CCTCAGC(-5/-2)
5826 GCTGAGG
BlpI 1113 GCTCAGC GC^T[ACGT]AGC
1520 GCTTAGC
Bpu1102I 1113 GCTCAGC GC^T[ACGT]AGC
1520 GCTTAGC
BsmBI 225 CGTCTC CGTCTC(1/5)
5245 GAGACG
BstUI 3849 CGCG CG^CG
3851 CGCG
CelII 1113 GCTCAGC GC^T[ACGT]AGC
1520 GCTTAGC
Eam1105I 296 GACTAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2550 GACATGGAGTC
HindIII 38 AAGCTT A^AGCTT
3103 AAGCTT
MvnI 3849 CGCG CG^CG
3851 CGCG
NheI 1035 GCTAGC G^CTAGC
2174 GCTAGC
PflFI 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5775 GACAGAGTC
PpuMI 2208 GGGACCT [GA]G^G[AT]CC[CT]
3237 GGGACCT
SapI 480 GAAGAGC GCTCTTC(1/4)
560 GCTCTTC
SbfI 5452 CCTGCAGG CCTGCA^GG
5755 CCTGCAGG
Sse8387I 5452 CCTGCAGG CCTGCA^GG
5755 CCTGCAGG
Tth111I 5271 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5775 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII