##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI AocI Aor51HI
AscI AseI AsnI Asp700I AvrII
BbvCI BclI BglI BlnI BsaI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eco47III Eco81I EcoRV EcoT22I FbaI
FseI HgaI HpaI KspI MluI
MslI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 490 CGCG CG^CG
AccIII 4358 TCCGGA T^CCGGA
AcyI 688 GGCGCC G[GA]^CG[CT]C
AflII 5477 CTTAAG C^TTAAG
Alw44I 3925 GTGCAC G^TGCAC
ApaLI 3925 GTGCAC G^TGCAC
AspI 577 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4910 GGATCC G^GATCC
BbeI 688 GGCGCC GGCGC^C
BcgI 277 CGACTATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5477 CTTAAG C^TTAAG
BsaBI 4983 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 688 GGCGCC G[GA]^CG[CT]C
BseAI 4358 TCCGGA T^CCGGA
BsiEI 2425 CGGCCG CG[GA][CT]^CG
BsmI 922 GAATGC GAATGC(1/-1)
BspEI 4358 TCCGGA T^CCGGA
BspHI 3939 TCATGA T^CATGA
BsrFI 2194 ACCGGC [GA]^CCGG[CT]
BstEII 4857 GGTAACC G^GT[ACGT]ACC
BstPI 4857 GGTAACC G^GT[ACGT]ACC
BstUI 490 CGCG CG^CG
Cfr10I 2194 ACCGGC [GA]^CCGG[CT]
DraIII 3257 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 912 CCGTGG C^C[GA][CT]GG
EagI 2425 CGGCCG C^GGCCG
EclXI 2425 CGGCCG C^GGCCG
Eco52I 2425 CGGCCG C^GGCCG
EcoO65I 4857 GGTAACC G^GT[ACGT]ACC
EcoRI 1021 GAATTC G^AATTC
HaeII 688 GGCGCC [GA]GCGC^[CT]
Hin1I 688 GGCGCC G[GA]^CG[CT]C
HindIII 4632 AAGCTT A^AGCTT
KasI 688 GGCGCC G^GCGCC
MamI 4983 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3987 CAATTG C^AATTG
MroI 4358 TCCGGA T^CCGGA
MunI 3987 CAATTG C^AATTG
MvnI 490 CGCG CG^CG
NarI 688 GGCGCC GG^CGCC
PflFI 577 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3939 TCATGA T^CATGA
SapI 3909 GAAGAGC GCTCTTC(1/4)
SfoI 688 GGCGCC GGC^GCC
SmaI 3857 CCCGGG CCC^GGG
SspI 4550 AATATT AAT^ATT
Tth111I 577 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3857 CCCGGG C^CCGGG
XmaI 3857 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2667 GGTACC G^GTACC
4327 GGTACC
AccI 3207 GTAGAC GT^[AC][GT]AC
3740 GTAGAC
AflIII 2202 ACATGT A^C[GA][CT]GT
5794 ACATGT
AhdI 464 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTAGGTGTC
ApaI 424 GGGCCC GGGCC^C
1242 GGGCCC
Asp718I 2667 GGTACC G^GTACC
4327 GGTACC
AspEI 464 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTAGGTGTC
AviII 16 TGCGCA TGC^GCA
1683 TGCGCA
BbrPI 4375 CACGTG CAC^GTG
4889 CACGTG
BglII 3865 AGATCT A^GATCT
4315 AGATCT
BsaAI 4375 CACGTG [CT]AC^GT[GA]
4889 CACGTG
BsaWI 1627 ACCGGA [AT]^CCGG[AT]
4358 TCCGGA
BspLU11I 2202 ACATGT A^CATGT
5794 ACATGT
BspMI 1559 GCAGGT ACCTGC(4/8)
1927 GCAGGT
BsrDI 294 GCAATG GCAATG(2/0)
2610 GCAATG
BssSI 161 CACGAG CACGAG(-5/-1)
329 CACGAG
BstXI 714 CCAACTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1513 CCAACAGCCTGG
DrdI 299 GACTACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2900 GACTGGGATGTC
Eam1105I 464 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2641 GACTAGGTGTC
FspI 16 TGCGCA TGC^GCA
1683 TGCGCA
KpnI 2667 GGTACC GGTAC^C
4327 GGTACC
NdeI 2672 CATATG CA^TATG
4562 CATATG
PaeR7I 46 CTCGAG C^TCGAG
1107 CTCGAG
PmaCI 4375 CACGTG CAC^GTG
4889 CACGTG
PmlI 4375 CACGTG CAC^GTG
4889 CACGTG
SacI 1606 GAGCTC GAGCT^C
3075 GAGCTC
ScaI 270 AGTACT AGT^ACT
5344 AGTACT
StuI 3175 AGGCCT AGG^CCT
3896 AGGCCT
XhoI 46 CTCGAG C^TCGAG
1107 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BsrBI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII