##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII BciVI BclI BsiEI BspMI
BssHII Bst1107I BstUI BstZ17I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FbaI FspI KspI
MfeI MluI MunI MvnI NcoI
NdeI NotI NruI NsiI PacI
PflMI PmeI PshAI PshBI PvuI
RsrII SacII SalI SgrAI SmaI
SnaBI SphI SspI SwaI Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2088 GGTACC G^GTACC
AccIII 4536 TCCGGA T^CCGGA
AclI 166 AACGTT AA^CGTT
AflII 2939 CTTAAG C^TTAAG
AflIII 6340 ACATGT A^C[GA][CT]GT
AgeI 1127 ACCGGT A^CCGGT
ApaI 3297 GGGCCC GGGCC^C
Asp718I 2088 GGTACC G^GTACC
AspI 550 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 613 CCTAGG C^CTAGG
BbeI 1457 GGCGCC GGCGC^C
BcgI 3124 CGAAATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2939 CTTAAG C^TTAAG
BlnI 613 CCTAGG C^CTAGG
BseAI 4536 TCCGGA T^CCGGA
BsiWI 864 CGTACG C^GTACG
BspEI 4536 TCCGGA T^CCGGA
BspHI 2899 TCATGA T^CATGA
BspLU11I 6340 ACATGT A^CATGT
BssSI 4814 CACGAG CACGAG(-5/-1)
BstBI 6423 TTCGAA TT^CGAA
DraI 5083 TTTAAA TTT^AAA
DraIII 902 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 270 CCACGG C^C[GA][CT]GG
EcoRV 3041 GATATC GAT^ATC
FseI 1998 GGCCGGCC GGCCGG^CC
HpaI 622 GTTAAC GTT^AAC
KasI 1457 GGCGCC G^GCGCC
KpnI 2088 GGTACC GGTAC^C
MroI 4536 TCCGGA T^CCGGA
NaeI 1999 GCCGGC GCC^GGC
NarI 1457 GGCGCC GG^CGCC
NgoMIV 1999 GCCGGC G^CCGGC
NspV 6423 TTCGAA TT^CGAA
PflFI 550 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1127 ACCGGT A^CCGGT
Psp1406I 166 AACGTT AA^CGTT
RcaI 2899 TCATGA T^CATGA
SexAI 4110 ACCTGGT A^CC[AT]GGT
SfiI 6477 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1457 GGCGCC GGC^GCC
SfuI 6423 TTCGAA TT^CGAA
SpeI 6532 ACTAGT A^CTAGT
Tth111I 550 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1457 GGCGCC G[GA]^CG[CT]C
5183 GACGCC
AocI 4936 CCTGAGG CC^T[ACGT]AGG
6145 CCTCAGG
BamHI 6658 GGATCC G^GATCC
6693 GGATCC
BbrPI 902 CACGTG CAC^GTG
2459 CACGTG
BglI 693 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6478 GCCAGTGGGGC
BlpI 3668 GCTCAGC GC^T[ACGT]AGC
4730 GCTCAGC
Bpu1102I 3668 GCTCAGC GC^T[ACGT]AGC
4730 GCTCAGC
BsaAI 902 CACGTG [CT]AC^GT[GA]
2459 CACGTG
BsaHI 1457 GGCGCC G[GA]^CG[CT]C
5183 GACGCC
Bsp1407I 3704 TGTACA T^GTACA
3970 TGTACA
BspDI 2612 ATCGAT AT^CGAT
4647 ATCGAT
BsrGI 3704 TGTACA T^GTACA
3970 TGTACA
BstAPI 5263 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5575 GCAGGCGCTGC
Bsu36I 4936 CCTGAGG CC^T[ACGT]AGG
6145 CCTCAGG
CelII 3668 GCTCAGC GC^T[ACGT]AGC
4730 GCTCAGC
ClaI 2612 ATCGAT AT^CGAT
4647 ATCGAT
Eco81I 4936 CCTGAGG CC^T[ACGT]AGG
6145 CCTCAGG
Hin1I 1457 GGCGCC G[GA]^CG[CT]C
5183 GACGCC
NheI 1310 GCTAGC G^CTAGC
2281 GCTAGC
PmaCI 902 CACGTG CAC^GTG
2459 CACGTG
PmlI 902 CACGTG CAC^GTG
2459 CACGTG
SbfI 2587 CCTGCAGG CCTGCA^GG
3838 CCTGCAGG
Sse8387I 2587 CCTGCAGG CCTGCA^GG
3838 CCTGCAGG
SspBI 3704 TGTACA T^GTACA
3970 TGTACA
StuI 4179 AGGCCT AGG^CCT
6036 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrDI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII
XmnI