##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII BamHI BbeI
BbrPI BcgI BfrI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoT22I FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NcoI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 513 GAATTC [GA]^AATT[CT]
ApaI 4081 GGGCCC GGGCC^C
ApoI 513 GAATTC [GA]^AATT[CT]
AseI 1282 ATTAAT AT^TAAT
AsnI 1282 ATTAAT AT^TAAT
AspI 619 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4061 CCCGAG C^[CT]CG[GA]G
BbsI 4528 GAAGAC GAAGAC(2/6)
BclI 1132 TGATCA T^GATCA
BglI 905 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 4528 GAAGAC GAAGAC(2/6)
BsaAI 4868 TACGTG [CT]AC^GT[GA]
BsoBI 4061 CCCGAG C^[CT]CG[GA]G
BspLU11I 3273 ACATGT A^CATGT
BstEII 314 GGTGACC G^GT[ACGT]ACC
BstPI 314 GGTGACC G^GT[ACGT]ACC
DraIII 3990 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 860 GACATAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 690 CCACGG C^C[GA][CT]GG
EcoO65I 314 GGTGACC G^GT[ACGT]ACC
EcoRI 513 GAATTC G^AATTC
EcoRV 557 GATATC GAT^ATC
FbaI 1132 TGATCA T^GATCA
HgaI 3326 GCGTC GACGC(5/10)
HindIII 3195 AAGCTT A^AGCTT
MaeII 4869 ACGT A^CGT
NaeI 3671 GCCGGC GCC^GGC
NgoMIV 3671 GCCGGC G^CCGGC
NheI 4033 GCTAGC G^CTAGC
NruI 736 TCGCGA TCG^CGA
PflFI 619 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1282 ATTAAT AT^TAAT
SacI 4892 GAGCTC GAGCT^C
SexAI 204 ACCTGGT A^CC[AT]GGT
SspI 2400 AATATT AAT^ATT
Tth111I 619 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 774 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 237 GTAGAC GT^[AC][GT]AC
1502 GTAGAC
AccII 737 CGCG CG^CG
1904 CGCG
AflIII 3273 ACATGT A^C[GA][CT]GT
4869 ACGTGT
AlwI 1368 GATCC GGATC(4/5)
1876 GATCC
Asp700I 1491 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2132 GAATGCCTTC
BanI 3607 GGCACC G^G[CT][GA]CC
5066 GGCACC
BbvCI 1107 CCTCAGC CCTCAGC(-5/-2)
2075 CCTCAGC
BglII 2634 AGATCT A^GATCT
3317 AGATCT
BsmI 1629 GAATGC GAATGC(1/-1)
2132 GAATGC
BsrDI 298 CATTGC GCAATG(2/0)
1997 CATTGC
BsrFI 2342 GCCGGT [GA]^CCGG[CT]
3671 GCCGGC
BstUI 737 CGCG CG^CG
1904 CGCG
CfoI 1934 GCGC GCG^C
5396 GCGC
Cfr10I 2342 GCCGGT [GA]^CCGG[CT]
3671 GCCGGC
DraI 406 TTTAAA TTT^AAA
5147 TTTAAA
HhaI 1934 GCGC GCG^C
5396 GCGC
HinP1I 1934 GCGC G^CGC
5396 GCGC
HincII 121 GTTGAC GT[CT]^[GA]AC
5251 GTTGAC
HindII 121 GTTGAC GT[CT]^[GA]AC
5251 GTTGAC
MvnI 737 CGCG CG^CG
1904 CGCG
NdeI 1916 CATATG CA^TATG
4925 CATATG
SpeI 472 ACTAGT A^CTAGT
5261 ACTAGT
SphI 1764 GCATGC GCATG^C
1977 GCATGC
XmnI 1491 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2132 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AfaI AhdI AluI AlwNI
AocI AspEI AspHI AvaII AvrII
BalI BanII BbvI BciVI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrBI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII Cfr13I DdeI DpnII
DraII EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII