################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AflII AscI AseI AsnI Asp700I BclI BfrI BglII BsiWI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DrdI EcoRI EcoRV FbaI FseI HpaI KspI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PmeI PshBI PvuI RsrII SacII SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 1490 GACGTC GACGT^C AccIII 1102 TCCGGA T^CCGGA AclI 147 AACGTT AA^CGTT AcsI 2151 AAATTT [GA]^AATT[CT] AgeI 2332 ACCGGT A^CCGGT Aor51HI 1021 AGCGCT AGC^GCT ApoI 2151 AAATTT [GA]^AATT[CT] AvrII 4326 CCTAGG C^CTAGG BbeI 5816 GGCGCC GGCGC^C BlnI 4326 CCTAGG C^CTAGG BlpI 4700 GCTTAGC GC^T[ACGT]AGC Bpu1102I 4700 GCTTAGC GC^T[ACGT]AGC BsaBI 5483 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1102 TCCGGA T^CCGGA BspDI 822 ATCGAT AT^CGAT BspEI 1102 TCCGGA T^CCGGA BspLU11I 5752 ACATGT A^CATGT CelII 4700 GCTTAGC GC^T[ACGT]AGC ClaI 822 ATCGAT AT^CGAT EagI 2033 CGGCCG C^GGCCG EclXI 2033 CGGCCG C^GGCCG Eco47III 1021 AGCGCT AGC^GCT Eco52I 2033 CGGCCG C^GGCCG HindIII 5598 AAGCTT A^AGCTT KasI 5816 GGCGCC G^GCGCC MamI 5483 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 6122 CAATTG C^AATTG MroI 1102 TCCGGA T^CCGGA MunI 6122 CAATTG C^AATTG NarI 5816 GGCGCC GG^CGCC NdeI 5666 CATATG CA^TATG PaeR7I 1732 CTCGAG C^TCGAG PinAI 2332 ACCGGT A^CCGGT Psp1406I 147 AACGTT AA^CGTT SalI 1791 GTCGAC G^TCGAC SfiI 567 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 5816 GGCGCC GGC^GCC XhoI 1732 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 867 GGTACC G^GTACC 1084 GGTACC AccI 1791 GTCGAC GT^[AC][GT]AC 3612 GTCTAC AhdI 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5055 GACTGCTGGTC AocI 3726 CCTCAGG CC^T[ACGT]AGG 4079 CCTGAGG Asp718I 867 GGTACC G^GTACC 1084 GGTACC AspEI 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5055 GACTGCTGGTC AspI 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4881 GACTGTGTC BamHI 2187 GGATCC G^GATCC 5784 GGATCC BbrPI 1698 CACGTG CAC^GTG 3669 CACGTG BcgI 2573 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3538 GCACAGTGGTCG BciVI 3770 GGATAC GTATCC(6/5) 6514 GGATAC BsiEI 2033 CGGCCG CG[GA][CT]^CG 4649 CGACCG BsmI 371 GAATGC GAATGC(1/-1) 527 GCATTC Bsp1407I 3082 TGTACA T^GTACA 5317 TGTACA BspHI 1330 TCATGA T^CATGA 2495 TCATGA BsrGI 3082 TGTACA T^GTACA 5317 TGTACA BstXI 4521 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4684 CCAAGCCAGTGG Bsu36I 3726 CCTCAGG CC^T[ACGT]AGG 4079 CCTGAGG DraI 5455 TTTAAA TTT^AAA 6651 TTTAAA Eam1105I 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5055 GACTGCTGGTC Eco81I 3726 CCTCAGG CC^T[ACGT]AGG 4079 CCTGAGG EcoT22I 803 ATGCAT ATGCA^T 6754 ATGCAT KpnI 867 GGTACC GGTAC^C 1084 GGTACC NsiI 803 ATGCAT ATGCA^T 6754 ATGCAT PflFI 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4881 GACTGTGTC PmaCI 1698 CACGTG CAC^GTG 3669 CACGTG PmlI 1698 CACGTG CAC^GTG 3669 CACGTG RcaI 1330 TCATGA T^CATGA 2495 TCATGA SapI 2757 GAAGAGC GCTCTTC(1/4) 2246 GCTCTTC ScaI 544 AGTACT AGT^ACT 1774 AGTACT SphI 805 GCATGC GCATG^C 3619 GCATGC SspBI 3082 TGTACA T^GTACA 5317 TGTACA Tth111I 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4881 GACTGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII