##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AflII AscI AseI AsnI Asp700I
BclI BfrI BglII BsiWI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
DrdI EcoRI EcoRV FbaI FseI
HpaI KspI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PmeI PshBI PvuI RsrII SacII
SbfI SexAI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1490 GACGTC GACGT^C
AccIII 1102 TCCGGA T^CCGGA
AclI 147 AACGTT AA^CGTT
AcsI 2151 AAATTT [GA]^AATT[CT]
AgeI 2332 ACCGGT A^CCGGT
Aor51HI 1021 AGCGCT AGC^GCT
ApoI 2151 AAATTT [GA]^AATT[CT]
AvrII 4326 CCTAGG C^CTAGG
BbeI 5816 GGCGCC GGCGC^C
BlnI 4326 CCTAGG C^CTAGG
BlpI 4700 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4700 GCTTAGC GC^T[ACGT]AGC
BsaBI 5483 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1102 TCCGGA T^CCGGA
BspDI 822 ATCGAT AT^CGAT
BspEI 1102 TCCGGA T^CCGGA
BspLU11I 5752 ACATGT A^CATGT
CelII 4700 GCTTAGC GC^T[ACGT]AGC
ClaI 822 ATCGAT AT^CGAT
EagI 2033 CGGCCG C^GGCCG
EclXI 2033 CGGCCG C^GGCCG
Eco47III 1021 AGCGCT AGC^GCT
Eco52I 2033 CGGCCG C^GGCCG
HindIII 5598 AAGCTT A^AGCTT
KasI 5816 GGCGCC G^GCGCC
MamI 5483 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6122 CAATTG C^AATTG
MroI 1102 TCCGGA T^CCGGA
MunI 6122 CAATTG C^AATTG
NarI 5816 GGCGCC GG^CGCC
NdeI 5666 CATATG CA^TATG
PaeR7I 1732 CTCGAG C^TCGAG
PinAI 2332 ACCGGT A^CCGGT
Psp1406I 147 AACGTT AA^CGTT
SalI 1791 GTCGAC G^TCGAC
SfiI 567 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 5816 GGCGCC GGC^GCC
XhoI 1732 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 867 GGTACC G^GTACC
1084 GGTACC
AccI 1791 GTCGAC GT^[AC][GT]AC
3612 GTCTAC
AhdI 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5055 GACTGCTGGTC
AocI 3726 CCTCAGG CC^T[ACGT]AGG
4079 CCTGAGG
Asp718I 867 GGTACC G^GTACC
1084 GGTACC
AspEI 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5055 GACTGCTGGTC
AspI 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4881 GACTGTGTC
BamHI 2187 GGATCC G^GATCC
5784 GGATCC
BbrPI 1698 CACGTG CAC^GTG
3669 CACGTG
BcgI 2573 CGAGGCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3538 GCACAGTGGTCG
BciVI 3770 GGATAC GTATCC(6/5)
6514 GGATAC
BsiEI 2033 CGGCCG CG[GA][CT]^CG
4649 CGACCG
BsmI 371 GAATGC GAATGC(1/-1)
527 GCATTC
Bsp1407I 3082 TGTACA T^GTACA
5317 TGTACA
BspHI 1330 TCATGA T^CATGA
2495 TCATGA
BsrGI 3082 TGTACA T^GTACA
5317 TGTACA
BstXI 4521 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4684 CCAAGCCAGTGG
Bsu36I 3726 CCTCAGG CC^T[ACGT]AGG
4079 CCTGAGG
DraI 5455 TTTAAA TTT^AAA
6651 TTTAAA
Eam1105I 2981 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5055 GACTGCTGGTC
Eco81I 3726 CCTCAGG CC^T[ACGT]AGG
4079 CCTGAGG
EcoT22I 803 ATGCAT ATGCA^T
6754 ATGCAT
KpnI 867 GGTACC GGTAC^C
1084 GGTACC
NsiI 803 ATGCAT ATGCA^T
6754 ATGCAT
PflFI 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4881 GACTGTGTC
PmaCI 1698 CACGTG CAC^GTG
3669 CACGTG
PmlI 1698 CACGTG CAC^GTG
3669 CACGTG
RcaI 1330 TCATGA T^CATGA
2495 TCATGA
SapI 2757 GAAGAGC GCTCTTC(1/4)
2246 GCTCTTC
ScaI 544 AGTACT AGT^ACT
1774 AGTACT
SphI 805 GCATGC GCATG^C
3619 GCATGC
SspBI 3082 TGTACA T^GTACA
5317 TGTACA
Tth111I 1431 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4881 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII