################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI AseI AsnI Asp700I Asp718I AspI BcgI BfrI BsiWI BspDI BspHI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EcoRI EcoRV KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 544 TCCGGA T^CCGGA AcsI 3177 GAATTT [GA]^AATT[CT] AflIII 3436 ACACGT A^C[GA][CT]GT ApoI 3177 GAATTT [GA]^AATT[CT] AvrII 4097 CCTAGG C^CTAGG BamHI 900 GGATCC G^GATCC BbeI 5308 GGCGCC GGCGC^C BbrPI 3648 CACGTG CAC^GTG BclI 2494 TGATCA T^GATCA BlnI 4097 CCTAGG C^CTAGG BsaBI 603 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 544 TCCGGA T^CCGGA BsiEI 2622 CGGCCG CG[GA][CT]^CG BsmI 590 GCATTC GAATGC(1/-1) BspEI 544 TCCGGA T^CCGGA BssHII 4208 GCGCGC G^CGCGC BssSI 4619 CACGAG CACGAG(-5/-1) EagI 2622 CGGCCG C^GGCCG EclXI 2622 CGGCCG C^GGCCG Eco52I 2622 CGGCCG C^GGCCG EcoT22I 422 ATGCAT ATGCA^T FbaI 2494 TGATCA T^GATCA FseI 1154 GGCCGGCC GGCCGG^CC HpaI 1517 GTTAAC GTT^AAC KasI 5308 GGCGCC G^GCGCC MamI 603 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 544 TCCGGA T^CCGGA NarI 5308 GGCGCC GG^CGCC NsiI 422 ATGCAT ATGCA^T PaeR7I 4111 CTCGAG C^TCGAG PmaCI 3648 CACGTG CAC^GTG PmlI 3648 CACGTG CAC^GTG PshAI 4528 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 5308 GGCGCC GGC^GCC SphI 5722 GCATGC GCATG^C XbaI 2324 TCTAGA T^CTAGA XhoI 4111 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1209 GTAGAC GT^[AC][GT]AC 2535 GTCTAC AhdI 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5430 GACATCCTGTC Aor51HI 2610 AGCGCT AGC^GCT 5476 AGCGCT AspEI 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5430 GACATCCTGTC AviII 1552 TGCGCA TGC^GCA 5747 TGCGCA BglII 1049 AGATCT A^GATCT 6198 AGATCT BsaAI 825 TACGTG [CT]AC^GT[GA] 3648 CACGTG Bsp1407I 178 TGTACA T^GTACA 1201 TGTACA BsrDI 4103 CATTGC GCAATG(2/0) 6045 CATTGC BsrGI 178 TGTACA T^GTACA 1201 TGTACA BstAPI 722 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 972 GCAGACCCTGC Eam1105I 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5430 GACATCCTGTC Eco47III 2610 AGCGCT AGC^GCT 5476 AGCGCT EcoNI 4092 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4505 CCTGGGCCAGG FspI 1552 TGCGCA TGC^GCA 5747 TGCGCA HindIII 434 AAGCTT A^AGCTT 3768 AAGCTT MseI 1518 TTAA T^TAA 6195 TTAA SbfI 978 CCTGCAGG CCTGCA^GG 3875 CCTGCAGG ScaI 649 AGTACT AGT^ACT 2239 AGTACT SfiI 3102 GGCCGCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4329 GGCCTCACAGGCC SmaI 1456 CCCGGG CCC^GGG 1966 CCCGGG Sse8387I 978 CCTGCAGG CCTGCA^GG 3875 CCTGCAGG SspBI 178 TGTACA T^GTACA 1201 TGTACA Tru9I 1518 TTAA T^TAA 6195 TTAA Tsp509I 1122 AATT ^AATT 3178 AATT XmaCI 1456 CCCGGG C^CCGGG 1966 CCCGGG XmaI 1456 CCCGGG C^CCGGG 1966 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII