##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AspI BcgI BfrI BsiWI BspDI
BspHI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EcoRI
EcoRV KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfuI SgrAI SnaBI
SpeI SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 544 TCCGGA T^CCGGA
AcsI 3177 GAATTT [GA]^AATT[CT]
AflIII 3436 ACACGT A^C[GA][CT]GT
ApoI 3177 GAATTT [GA]^AATT[CT]
AvrII 4097 CCTAGG C^CTAGG
BamHI 900 GGATCC G^GATCC
BbeI 5308 GGCGCC GGCGC^C
BbrPI 3648 CACGTG CAC^GTG
BclI 2494 TGATCA T^GATCA
BlnI 4097 CCTAGG C^CTAGG
BsaBI 603 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 544 TCCGGA T^CCGGA
BsiEI 2622 CGGCCG CG[GA][CT]^CG
BsmI 590 GCATTC GAATGC(1/-1)
BspEI 544 TCCGGA T^CCGGA
BssHII 4208 GCGCGC G^CGCGC
BssSI 4619 CACGAG CACGAG(-5/-1)
EagI 2622 CGGCCG C^GGCCG
EclXI 2622 CGGCCG C^GGCCG
Eco52I 2622 CGGCCG C^GGCCG
EcoT22I 422 ATGCAT ATGCA^T
FbaI 2494 TGATCA T^GATCA
FseI 1154 GGCCGGCC GGCCGG^CC
HpaI 1517 GTTAAC GTT^AAC
KasI 5308 GGCGCC G^GCGCC
MamI 603 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 544 TCCGGA T^CCGGA
NarI 5308 GGCGCC GG^CGCC
NsiI 422 ATGCAT ATGCA^T
PaeR7I 4111 CTCGAG C^TCGAG
PmaCI 3648 CACGTG CAC^GTG
PmlI 3648 CACGTG CAC^GTG
PshAI 4528 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 5308 GGCGCC GGC^GCC
SphI 5722 GCATGC GCATG^C
XbaI 2324 TCTAGA T^CTAGA
XhoI 4111 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1209 GTAGAC GT^[AC][GT]AC
2535 GTCTAC
AhdI 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5430 GACATCCTGTC
Aor51HI 2610 AGCGCT AGC^GCT
5476 AGCGCT
AspEI 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5430 GACATCCTGTC
AviII 1552 TGCGCA TGC^GCA
5747 TGCGCA
BglII 1049 AGATCT A^GATCT
6198 AGATCT
BsaAI 825 TACGTG [CT]AC^GT[GA]
3648 CACGTG
Bsp1407I 178 TGTACA T^GTACA
1201 TGTACA
BsrDI 4103 CATTGC GCAATG(2/0)
6045 CATTGC
BsrGI 178 TGTACA T^GTACA
1201 TGTACA
BstAPI 722 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
972 GCAGACCCTGC
Eam1105I 920 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5430 GACATCCTGTC
Eco47III 2610 AGCGCT AGC^GCT
5476 AGCGCT
EcoNI 4092 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4505 CCTGGGCCAGG
FspI 1552 TGCGCA TGC^GCA
5747 TGCGCA
HindIII 434 AAGCTT A^AGCTT
3768 AAGCTT
MseI 1518 TTAA T^TAA
6195 TTAA
SbfI 978 CCTGCAGG CCTGCA^GG
3875 CCTGCAGG
ScaI 649 AGTACT AGT^ACT
2239 AGTACT
SfiI 3102 GGCCGCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4329 GGCCTCACAGGCC
SmaI 1456 CCCGGG CCC^GGG
1966 CCCGGG
Sse8387I 978 CCTGCAGG CCTGCA^GG
3875 CCTGCAGG
SspBI 178 TGTACA T^GTACA
1201 TGTACA
Tru9I 1518 TTAA T^TAA
6195 TTAA
Tsp509I 1122 AATT ^AATT
3178 AATT
XmaCI 1456 CCCGGG C^CCGGG
1966 CCCGGG
XmaI 1456 CCCGGG C^CCGGG
1966 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII