##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII BbrPI
BcgI BclI BfrI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco52I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1828 GGTACC G^GTACC
AocI 3754 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1485 AGCGCT AGC^GCT
ApaI 4116 GGGCCC GGGCC^C
Asp700I 3248 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1828 GGTACC G^GTACC
AspI 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 154 GGCGCC GGCGC^C
BciVI 1584 GTATCC GTATCC(6/5)
BsaAI 2222 TACGTG [CT]AC^GT[GA]
BsaWI 467 TCCGGT [AT]^CCGG[AT]
BsgI 802 GTGCAG GTGCAG(16/14)
BsmI 4457 GCATTC GAATGC(1/-1)
BssHII 1030 GCGCGC G^CGCGC
Bsu36I 3754 CCTGAGG CC^T[ACGT]AGG
DsaI 32 CCGTGG C^C[GA][CT]GG
Eco47III 1485 AGCGCT AGC^GCT
Eco81I 3754 CCTGAGG CC^T[ACGT]AGG
EcoRI 1843 GAATTC G^AATTC
EcoRV 2536 GATATC GAT^ATC
KasI 154 GGCGCC G^GCGCC
KpnI 1828 GGTACC GGTAC^C
NarI 154 GGCGCC GG^CGCC
NheI 2657 GCTAGC G^CTAGC
PflFI 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 4716 GAGCTC GAGCT^C
ScaI 4092 AGTACT AGT^ACT
SfiI 262 GGCCGGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 154 GGCGCC GGC^GCC
SpeI 1596 ACTAGT A^CTAGT
SphI 5087 GCATGC GCATG^C
Tth111I 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3248 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 430 GTCTAC GT^[AC][GT]AC
685 GTAGAC
AcyI 154 GGCGCC G[GA]^CG[CT]C
4262 GGCGTC
AflIII 1082 ACATGT A^C[GA][CT]GT
4408 ACATGT
AhdI 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2962 GACTCAGAGTC
Alw44I 226 GTGCAC G^TGCAC
659 GTGCAC
ApaLI 226 GTGCAC G^TGCAC
659 GTGCAC
AspEI 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2962 GACTCAGAGTC
BglII 3785 AGATCT A^GATCT
4939 AGATCT
BlpI 3923 GCTGAGC GC^T[ACGT]AGC
4137 GCTCAGC
Bpu1102I 3923 GCTGAGC GC^T[ACGT]AGC
4137 GCTCAGC
BsaHI 154 GGCGCC G[GA]^CG[CT]C
4262 GGCGTC
BspLU11I 1082 ACATGT A^CATGT
4408 ACATGT
BsrBI 346 GAGCGG CCGCTC(-3/-3)
3111 GAGCGG
BsrDI 3648 CATTGC GCAATG(2/0)
5815 CATTGC
BstEII 1132 GGTCACC G^GT[ACGT]ACC
2362 GGTCACC
BstPI 1132 GGTCACC G^GT[ACGT]ACC
2362 GGTCACC
BstXI 2217 CCAAATACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3781 CCACAGATCTGG
CelII 3923 GCTGAGC GC^T[ACGT]AGC
4137 GCTCAGC
DraIII 3932 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5632 CACACCGTG
Eam1105I 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2962 GACTCAGAGTC
EcoO65I 1132 GGTCACC G^GT[ACGT]ACC
2362 GGTCACC
Hin1I 154 GGCGCC G[GA]^CG[CT]C
4262 GGCGTC
HindIII 3250 AAGCTT A^AGCTT
3986 AAGCTT
PflMI 480 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2608 CCAGCACTTGG
PshAI 5 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5239 GACAACTGTC
SapI 4626 GAAGAGC GCTCTTC(1/4)
5332 GCTCTTC
StuI 4524 AGGCCT AGG^CCT
5079 AGGCCT
TaqI 620 TCGA T^CGA
4146 TCGA
TthHB8I 620 TCGA T^CGA
4146 TCGA
Van91I 480 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2608 CCAGCACTTGG
XbaI 4056 TCTAGA T^CTAGA
4271 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmaCI
XmaI