################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccIII AclI AflII AgeI AscI AseI AsnI AviII BbrPI BcgI BclI BfrI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 1828 GGTACC G^GTACC AocI 3754 CCTGAGG CC^T[ACGT]AGG Aor51HI 1485 AGCGCT AGC^GCT ApaI 4116 GGGCCC GGGCC^C Asp700I 3248 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1828 GGTACC G^GTACC AspI 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 154 GGCGCC GGCGC^C BciVI 1584 GTATCC GTATCC(6/5) BsaAI 2222 TACGTG [CT]AC^GT[GA] BsaWI 467 TCCGGT [AT]^CCGG[AT] BsgI 802 GTGCAG GTGCAG(16/14) BsmI 4457 GCATTC GAATGC(1/-1) BssHII 1030 GCGCGC G^CGCGC Bsu36I 3754 CCTGAGG CC^T[ACGT]AGG DsaI 32 CCGTGG C^C[GA][CT]GG Eco47III 1485 AGCGCT AGC^GCT Eco81I 3754 CCTGAGG CC^T[ACGT]AGG EcoRI 1843 GAATTC G^AATTC EcoRV 2536 GATATC GAT^ATC KasI 154 GGCGCC G^GCGCC KpnI 1828 GGTACC GGTAC^C NarI 154 GGCGCC GG^CGCC NheI 2657 GCTAGC G^CTAGC PflFI 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 4716 GAGCTC GAGCT^C ScaI 4092 AGTACT AGT^ACT SfiI 262 GGCCGGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 154 GGCGCC GGC^GCC SpeI 1596 ACTAGT A^CTAGT SphI 5087 GCATGC GCATG^C Tth111I 511 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3248 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 430 GTCTAC GT^[AC][GT]AC 685 GTAGAC AcyI 154 GGCGCC G[GA]^CG[CT]C 4262 GGCGTC AflIII 1082 ACATGT A^C[GA][CT]GT 4408 ACATGT AhdI 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2962 GACTCAGAGTC Alw44I 226 GTGCAC G^TGCAC 659 GTGCAC ApaLI 226 GTGCAC G^TGCAC 659 GTGCAC AspEI 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2962 GACTCAGAGTC BglII 3785 AGATCT A^GATCT 4939 AGATCT BlpI 3923 GCTGAGC GC^T[ACGT]AGC 4137 GCTCAGC Bpu1102I 3923 GCTGAGC GC^T[ACGT]AGC 4137 GCTCAGC BsaHI 154 GGCGCC G[GA]^CG[CT]C 4262 GGCGTC BspLU11I 1082 ACATGT A^CATGT 4408 ACATGT BsrBI 346 GAGCGG CCGCTC(-3/-3) 3111 GAGCGG BsrDI 3648 CATTGC GCAATG(2/0) 5815 CATTGC BstEII 1132 GGTCACC G^GT[ACGT]ACC 2362 GGTCACC BstPI 1132 GGTCACC G^GT[ACGT]ACC 2362 GGTCACC BstXI 2217 CCAAATACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3781 CCACAGATCTGG CelII 3923 GCTGAGC GC^T[ACGT]AGC 4137 GCTCAGC DraIII 3932 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5632 CACACCGTG Eam1105I 2237 GACTCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2962 GACTCAGAGTC EcoO65I 1132 GGTCACC G^GT[ACGT]ACC 2362 GGTCACC Hin1I 154 GGCGCC G[GA]^CG[CT]C 4262 GGCGTC HindIII 3250 AAGCTT A^AGCTT 3986 AAGCTT PflMI 480 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2608 CCAGCACTTGG PshAI 5 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5239 GACAACTGTC SapI 4626 GAAGAGC GCTCTTC(1/4) 5332 GCTCTTC StuI 4524 AGGCCT AGG^CCT 5079 AGGCCT TaqI 620 TCGA T^CGA 4146 TCGA TthHB8I 620 TCGA T^CGA 4146 TCGA Van91I 480 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2608 CCAGCACTTGG XbaI 4056 TCTAGA T^CTAGA 4271 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI