##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AscI AvrII BbeI BciVI
BfrI BlnI BsaHI BseAI BsiEI
BsiWI BsmI Bsp1407I BspEI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI EagI EclXI Eco52I EcoO65I
EcoRV Hin1I HindIII HpaI KasI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PmeI
Psp1406I PvuI RsrII SalI SapI
SbfI SfiI SfoI SfuI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 4965 GAATTC [GA]^AATT[CT]
Aor51HI 3174 AGCGCT AGC^GCT
ApoI 4965 GAATTC [GA]^AATT[CT]
AseI 5716 ATTAAT AT^TAAT
AsnI 5716 ATTAAT AT^TAAT
AspI 289 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 2516 TGATCA T^GATCA
BlpI 3282 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3282 GCTGAGC GC^T[ACGT]AGC
BspDI 227 ATCGAT AT^CGAT
CelII 3282 GCTGAGC GC^T[ACGT]AGC
ClaI 227 ATCGAT AT^CGAT
Eco47III 3174 AGCGCT AGC^GCT
EcoRI 4965 GAATTC G^AATTC
FbaI 2516 TGATCA T^GATCA
FseI 922 GGCCGGCC GGCCGG^CC
HgaI 1987 GCGTC GACGC(5/10)
NaeI 923 GCCGGC GCC^GGC
NgoMIV 923 GCCGGC G^CCGGC
NheI 5465 GCTAGC G^CTAGC
PaeR7I 3009 CTCGAG C^TCGAG
PflFI 289 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 897 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3298 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5716 ATTAAT AT^TAAT
SgrAI 5073 CACCGGTG C[GA]^CCGG[CT]G
SmaI 5771 CCCGGG CCC^GGG
SnaBI 6268 TACGTA TAC^GTA
SphI 529 GCATGC GCATG^C
Tth111I 289 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 897 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 6136 TCTAGA T^CTAGA
XhoI 3009 CTCGAG C^TCGAG
XmaCI 5771 CCCGGG C^CCGGG
XmaI 5771 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AgeI 5074 ACCGGT A^CCGGT
5185 ACCGGT
AhdI 487 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3072 GACCCCTAGTC
AspEI 487 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3072 GACCCCTAGTC
AviII 532 TGCGCA TGC^GCA
6170 TGCGCA
BamHI 1128 GGATCC G^GATCC
1683 GGATCC
BbrPI 1696 CACGTG CAC^GTG
5219 CACGTG
BcgI 3011 CGAGTACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6409 CGACAATGCTGC
BsaBI 2383 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4123 GATTCTCATC
DrdI 330 GACCTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3469 GACTTCAGAGTC
Eam1105I 487 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3072 GACCCCTAGTC
EcoT22I 5054 ATGCAT ATGCA^T
5637 ATGCAT
FspI 532 TGCGCA TGC^GCA
6170 TGCGCA
HincII 3720 GTCAAC GT[CT]^[GA]AC
4043 GTCAAC
HindII 3720 GTCAAC GT[CT]^[GA]AC
4043 GTCAAC
KspI 49 CCGCGG CCGC^GG
96 CCGCGG
MamI 2383 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4123 GATTCTCATC
NdeI 3102 CATATG CA^TATG
3944 CATATG
NsiI 5054 ATGCAT ATGCA^T
5637 ATGCAT
PinAI 5074 ACCGGT A^CCGGT
5185 ACCGGT
PmaCI 1696 CACGTG CAC^GTG
5219 CACGTG
PmlI 1696 CACGTG CAC^GTG
5219 CACGTG
SacII 49 CCGCGG CCGC^GG
96 CCGCGG
ScaI 344 AGTACT AGT^ACT
3013 AGTACT
SpeI 2262 ACTAGT A^CTAGT
6296 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI Asp700I Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI