##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BalI BbeI BbrPI BcgI
BcnI BglI BlnI BsaAI BsaHI
BsiEI BsiWI BsmBI BsoBI BspDI
BsrBI BsrFI BssHII BstEII BstPI
BstUI BstXI CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MluI MluNI
MscI MvnI NaeI NarI NciI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 1859 TCCGGA T^CCGGA
AflII 3681 CTTAAG C^TTAAG
ApaI 2319 GGGCCC GGGCC^C
AseI 4049 ATTAAT AT^TAAT
AsnI 4049 ATTAAT AT^TAAT
AspI 5197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1785 CCTCAGC CCTCAGC(-5/-2)
BfrI 3681 CTTAAG C^TTAAG
BsaBI 1539 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1859 TCCGGA [AT]^CCGG[AT]
BseAI 1859 TCCGGA T^CCGGA
BspEI 1859 TCCGGA T^CCGGA
BssSI 3283 CACGAG CACGAG(-5/-1)
Bst1107I 4450 GTATAC GTA^TAC
BstBI 4663 TTCGAA TT^CGAA
BstZ17I 4450 GTATAC GTA^TAC
DraIII 2810 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3588 TGGCCG [CT]^GGCC[GA]
HapII 1860 CCGG C^CGG
HpaI 2818 GTTAAC GTT^AAC
HpaII 1860 CCGG C^CGG
MaeII 6127 ACGT A^CGT
MamI 1539 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5766 CAATTG C^AATTG
MroI 1859 TCCGGA T^CCGGA
MspI 1860 CCGG C^CGG
MunI 5766 CAATTG C^AATTG
NspV 4663 TTCGAA TT^CGAA
PflFI 5197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 167 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4049 ATTAAT AT^TAAT
SfuI 4663 TTCGAA TT^CGAA
Tth111I 5197 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 167 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 61 ACATGT A^C[GA][CT]GT
5049 ACATGT
Asp700I 4438 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5031 GAAGTTTTTC
BciVI 296 GTATCC GTATCC(6/5)
3347 GTATCC
BsaI 3149 GAGACC GGTCTC(1/5)
4549 GGTCTC
Bsp1407I 3736 TGTACA T^GTACA
5446 TGTACA
BspLU11I 61 ACATGT A^CATGT
5049 ACATGT
BsrGI 3736 TGTACA T^GTACA
5446 TGTACA
DraI 3008 TTTAAA TTT^AAA
5024 TTTAAA
DsaI 1585 CCATGG C^C[GA][CT]GG
1954 CCATGG
EcoT22I 4421 ATGCAT ATGCA^T
5453 ATGCAT
FauI 2007 CCCGC CCCGC(4/6)
3388 GCGGG
HincII 660 GTTGAC GT[CT]^[GA]AC
2818 GTTAAC
HindII 660 GTTGAC GT[CT]^[GA]AC
2818 GTTAAC
NcoI 1585 CCATGG C^CATGG
1954 CCATGG
NdeI 381 CATATG CA^TATG
2956 CATATG
NsiI 4421 ATGCAT ATGCA^T
5453 ATGCAT
PshAI 1356 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5048 GACATGTGTC
SapI 357 GAAGAGC GCTCTTC(1/4)
636 GCTCTTC
SpeI 531 ACTAGT A^CTAGT
4057 ACTAGT
SphI 2380 GCATGC GCATG^C
4419 GCATGC
SspBI 3736 TGTACA T^GTACA
5446 TGTACA
SspI 1072 AATATT AAT^ATT
4872 AATATT
TaqI 4664 TCGA T^CGA
5380 TCGA
TthHB8I 4664 TCGA T^CGA
5380 TCGA
XbaI 2882 TCTAGA T^CTAGA
3699 TCTAGA
XmnI 4438 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5031 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BamHI BanI BanII BbsI
BbvI BclI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraII
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NheI
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I SbfI ScaI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII