################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BcgI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BstEII BstPI ClaI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI KpnI MamI MluI MroI NdeI NotI NruI PacI PaeR7I PinAI PmeI PshBI PvuI SalI SexAI SmaI SnaBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AclI 2740 AACGTT AA^CGTT Alw44I 4765 GTGCAC G^TGCAC AlwNI 965 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4795 CCTCAGG CC^T[ACGT]AGG ApaLI 4765 GTGCAC G^TGCAC Asp700I 2632 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1141 TGGCCA TGG^CCA BbeI 942 GGCGCC GGCGC^C BbrPI 759 CACGTG CAC^GTG BglII 1164 AGATCT A^GATCT BlpI 2007 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2007 GCTGAGC GC^T[ACGT]AGC BsrBI 2010 GAGCGG CCGCTC(-3/-3) BssHII 1 GCGCGC G^CGCGC BssSI 847 CACGAG CACGAG(-5/-1) Bst1107I 1313 GTATAC GTA^TAC BstBI 1154 TTCGAA TT^CGAA BstZ17I 1313 GTATAC GTA^TAC Bsu36I 4795 CCTCAGG CC^T[ACGT]AGG CelII 2007 GCTGAGC GC^T[ACGT]AGC CpoI 16 CGGTCCG CG^G[AT]CCG Eco81I 4795 CCTCAGG CC^T[ACGT]AGG EcoRI 1051 GAATTC G^AATTC KasI 942 GGCGCC G^GCGCC KspI 96 CCGCGG CCGC^GG MfeI 1983 CAATTG C^AATTG MluNI 1141 TGGCCA TGG^CCA MscI 1141 TGGCCA TGG^CCA MunI 1983 CAATTG C^AATTG NaeI 2159 GCCGGC GCC^GGC NarI 942 GGCGCC GG^CGCC NcoI 4145 CCATGG C^CATGG NgoMIV 2159 GCCGGC G^CCGGC NspV 1154 TTCGAA TT^CGAA PflFI 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 759 CACGTG CAC^GTG PmlI 759 CACGTG CAC^GTG PshAI 3095 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2740 AACGTT AA^CGTT RsrII 16 CGGTCCG CG^G[AT]CCG SacI 4668 GAGCTC GAGCT^C SacII 96 CCGCGG CCGC^GG SapI 2564 GAAGAGC GCTCTTC(1/4) SbfI 1174 CCTGCAGG CCTGCA^GG SfiI 190 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 942 GGCGCC GGC^GCC SfuI 1154 TTCGAA TT^CGAA SgrAI 4471 CGCCGGTG C[GA]^CCGG[CT]G SpeI 735 ACTAGT A^CTAGT SphI 5591 GCATGC GCATG^C Sse8387I 1174 CCTGCAGG CCTGCA^GG Tth111I 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 4295 CCAGCCCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2632 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 942 GGCGCC G[GA]^CG[CT]C 4469 GACGCC AflII 2254 CTTAAG C^TTAAG 2982 CTTAAG ApaI 69 GGGCCC GGGCC^C 85 GGGCCC AvaI 3806 CCCGAG C^[CT]CG[GA]G 5611 CTCGGG AvrII 43 CCTAGG C^CTAGG 3164 CCTAGG BamHI 1682 GGATCC G^GATCC 4250 GGATCC BanI 942 GGCGCC G^G[CT][GA]CC 3115 GGTGCC BciVI 3135 GGATAC GTATCC(6/5) 4112 GTATCC BclI 572 TGATCA T^GATCA 5179 TGATCA BfrI 2254 CTTAAG C^TTAAG 2982 CTTAAG BglI 142 GCCCTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 191 GCCGCCGGGGC BlnI 43 CCTAGG C^CTAGG 3164 CCTAGG BsaAI 759 CACGTG [CT]AC^GT[GA] 4288 TACGTG BsaHI 942 GGCGCC G[GA]^CG[CT]C 4469 GACGCC BsaI 2116 GAGACC GGTCTC(1/5) 3101 GGTCTC BsgI 715 CTGCAC GTGCAG(16/14) 793 GTGCAG BsmI 300 GCATTC GAATGC(1/-1) 3060 GCATTC BsoBI 3806 CCCGAG C^[CT]CG[GA]G 5611 CTCGGG BspHI 765 TCATGA T^CATGA 1041 TCATGA BspLU11I 2962 ACATGT A^CATGT 5447 ACATGT BsrDI 2164 CATTGC GCAATG(2/0) 5259 GCAATG BstAPI 786 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1979 GCAGCAATTGC BstXI 782 CCACGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4733 CCAGCATAATGG DsaI 96 CCGCGG C^C[GA][CT]GG 4145 CCATGG EcoT22I 3601 ATGCAT ATGCA^T 4256 ATGCAT FbaI 572 TGATCA T^GATCA 5179 TGATCA Hin1I 942 GGCGCC G[GA]^CG[CT]C 4469 GACGCC NheI 4266 GCTAGC G^CTAGC 4858 GCTAGC NsiI 3601 ATGCAT ATGCA^T 4256 ATGCAT PpuMI 1667 AGGACCT [GA]G^G[AT]CC[CT] 2815 GGGACCT PstI 1175 CTGCAG CTGCA^G 2152 CTGCAG PvuII 1996 CAGCTG CAG^CTG 2346 CAGCTG RcaI 765 TCATGA T^CATGA 1041 TCATGA StuI 4028 AGGCCT AGG^CCT 4236 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII