##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII BcgI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BstEII BstPI ClaI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
KpnI MamI MluI MroI NdeI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI PvuI SalI SexAI
SmaI SnaBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 2740 AACGTT AA^CGTT
Alw44I 4765 GTGCAC G^TGCAC
AlwNI 965 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4795 CCTCAGG CC^T[ACGT]AGG
ApaLI 4765 GTGCAC G^TGCAC
Asp700I 2632 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1141 TGGCCA TGG^CCA
BbeI 942 GGCGCC GGCGC^C
BbrPI 759 CACGTG CAC^GTG
BglII 1164 AGATCT A^GATCT
BlpI 2007 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2007 GCTGAGC GC^T[ACGT]AGC
BsrBI 2010 GAGCGG CCGCTC(-3/-3)
BssHII 1 GCGCGC G^CGCGC
BssSI 847 CACGAG CACGAG(-5/-1)
Bst1107I 1313 GTATAC GTA^TAC
BstBI 1154 TTCGAA TT^CGAA
BstZ17I 1313 GTATAC GTA^TAC
Bsu36I 4795 CCTCAGG CC^T[ACGT]AGG
CelII 2007 GCTGAGC GC^T[ACGT]AGC
CpoI 16 CGGTCCG CG^G[AT]CCG
Eco81I 4795 CCTCAGG CC^T[ACGT]AGG
EcoRI 1051 GAATTC G^AATTC
KasI 942 GGCGCC G^GCGCC
KspI 96 CCGCGG CCGC^GG
MfeI 1983 CAATTG C^AATTG
MluNI 1141 TGGCCA TGG^CCA
MscI 1141 TGGCCA TGG^CCA
MunI 1983 CAATTG C^AATTG
NaeI 2159 GCCGGC GCC^GGC
NarI 942 GGCGCC GG^CGCC
NcoI 4145 CCATGG C^CATGG
NgoMIV 2159 GCCGGC G^CCGGC
NspV 1154 TTCGAA TT^CGAA
PflFI 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 759 CACGTG CAC^GTG
PmlI 759 CACGTG CAC^GTG
PshAI 3095 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2740 AACGTT AA^CGTT
RsrII 16 CGGTCCG CG^G[AT]CCG
SacI 4668 GAGCTC GAGCT^C
SacII 96 CCGCGG CCGC^GG
SapI 2564 GAAGAGC GCTCTTC(1/4)
SbfI 1174 CCTGCAGG CCTGCA^GG
SfiI 190 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 942 GGCGCC GGC^GCC
SfuI 1154 TTCGAA TT^CGAA
SgrAI 4471 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 735 ACTAGT A^CTAGT
SphI 5591 GCATGC GCATG^C
Sse8387I 1174 CCTGCAGG CCTGCA^GG
Tth111I 5096 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4295 CCAGCCCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2632 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 942 GGCGCC G[GA]^CG[CT]C
4469 GACGCC
AflII 2254 CTTAAG C^TTAAG
2982 CTTAAG
ApaI 69 GGGCCC GGGCC^C
85 GGGCCC
AvaI 3806 CCCGAG C^[CT]CG[GA]G
5611 CTCGGG
AvrII 43 CCTAGG C^CTAGG
3164 CCTAGG
BamHI 1682 GGATCC G^GATCC
4250 GGATCC
BanI 942 GGCGCC G^G[CT][GA]CC
3115 GGTGCC
BciVI 3135 GGATAC GTATCC(6/5)
4112 GTATCC
BclI 572 TGATCA T^GATCA
5179 TGATCA
BfrI 2254 CTTAAG C^TTAAG
2982 CTTAAG
BglI 142 GCCCTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
191 GCCGCCGGGGC
BlnI 43 CCTAGG C^CTAGG
3164 CCTAGG
BsaAI 759 CACGTG [CT]AC^GT[GA]
4288 TACGTG
BsaHI 942 GGCGCC G[GA]^CG[CT]C
4469 GACGCC
BsaI 2116 GAGACC GGTCTC(1/5)
3101 GGTCTC
BsgI 715 CTGCAC GTGCAG(16/14)
793 GTGCAG
BsmI 300 GCATTC GAATGC(1/-1)
3060 GCATTC
BsoBI 3806 CCCGAG C^[CT]CG[GA]G
5611 CTCGGG
BspHI 765 TCATGA T^CATGA
1041 TCATGA
BspLU11I 2962 ACATGT A^CATGT
5447 ACATGT
BsrDI 2164 CATTGC GCAATG(2/0)
5259 GCAATG
BstAPI 786 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1979 GCAGCAATTGC
BstXI 782 CCACGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4733 CCAGCATAATGG
DsaI 96 CCGCGG C^C[GA][CT]GG
4145 CCATGG
EcoT22I 3601 ATGCAT ATGCA^T
4256 ATGCAT
FbaI 572 TGATCA T^GATCA
5179 TGATCA
Hin1I 942 GGCGCC G[GA]^CG[CT]C
4469 GACGCC
NheI 4266 GCTAGC G^CTAGC
4858 GCTAGC
NsiI 3601 ATGCAT ATGCA^T
4256 ATGCAT
PpuMI 1667 AGGACCT [GA]G^G[AT]CC[CT]
2815 GGGACCT
PstI 1175 CTGCAG CTGCA^G
2152 CTGCAG
PvuII 1996 CAGCTG CAG^CTG
2346 CAGCTG
RcaI 765 TCATGA T^CATGA
1041 TCATGA
StuI 4028 AGGCCT AGG^CCT
4236 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII