##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AviII       BcgI        BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BstEII      BstPI       ClaI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       FseI        FspI        
KpnI        MamI        MluI        MroI        NdeI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PshBI       PvuI        SalI        SexAI       
SmaI        SnaBI       SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AclI         2740  AACGTT               AA^CGTT
Alw44I       4765  GTGCAC               G^TGCAC
AlwNI         965  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         4795  CCTCAGG              CC^T[ACGT]AGG
ApaLI        4765  GTGCAC               G^TGCAC
Asp700I      2632  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         5096  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1141  TGGCCA               TGG^CCA
BbeI          942  GGCGCC               GGCGC^C
BbrPI         759  CACGTG               CAC^GTG
BglII        1164  AGATCT               A^GATCT
BlpI         2007  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2007  GCTGAGC              GC^T[ACGT]AGC
BsrBI        2010  GAGCGG               CCGCTC(-3/-3)
BssHII          1  GCGCGC               G^CGCGC
BssSI         847  CACGAG               CACGAG(-5/-1)
Bst1107I     1313  GTATAC               GTA^TAC
BstBI        1154  TTCGAA               TT^CGAA
BstZ17I      1313  GTATAC               GTA^TAC
Bsu36I       4795  CCTCAGG              CC^T[ACGT]AGG
CelII        2007  GCTGAGC              GC^T[ACGT]AGC
CpoI           16  CGGTCCG              CG^G[AT]CCG
Eco81I       4795  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1051  GAATTC               G^AATTC
KasI          942  GGCGCC               G^GCGCC
KspI           96  CCGCGG               CCGC^GG
MfeI         1983  CAATTG               C^AATTG
MluNI        1141  TGGCCA               TGG^CCA
MscI         1141  TGGCCA               TGG^CCA
MunI         1983  CAATTG               C^AATTG
NaeI         2159  GCCGGC               GCC^GGC
NarI          942  GGCGCC               GG^CGCC
NcoI         4145  CCATGG               C^CATGG
NgoMIV       2159  GCCGGC               G^CCGGC
NspV         1154  TTCGAA               TT^CGAA
PflFI        5096  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         759  CACGTG               CAC^GTG
PmlI          759  CACGTG               CAC^GTG
PshAI        3095  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2740  AACGTT               AA^CGTT
RsrII          16  CGGTCCG              CG^G[AT]CCG
SacI         4668  GAGCTC               GAGCT^C
SacII          96  CCGCGG               CCGC^GG
SapI         2564  GAAGAGC              GCTCTTC(1/4)
SbfI         1174  CCTGCAGG             CCTGCA^GG
SfiI          190  GGCCGCCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          942  GGCGCC               GGC^GCC
SfuI         1154  TTCGAA               TT^CGAA
SgrAI        4471  CGCCGGTG             C[GA]^CCGG[CT]G
SpeI          735  ACTAGT               A^CTAGT
SphI         5591  GCATGC               GCATG^C
Sse8387I     1174  CCTGCAGG             CCTGCA^GG
Tth111I      5096  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         4295  CCAGCCCTAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2632  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcyI          942  GGCGCC               G[GA]^CG[CT]C
             4469  GACGCC              
AflII        2254  CTTAAG               C^TTAAG
             2982  CTTAAG              
ApaI           69  GGGCCC               GGGCC^C
               85  GGGCCC              
AvaI         3806  CCCGAG               C^[CT]CG[GA]G
             5611  CTCGGG              
AvrII          43  CCTAGG               C^CTAGG
             3164  CCTAGG              
BamHI        1682  GGATCC               G^GATCC
             4250  GGATCC              
BanI          942  GGCGCC               G^G[CT][GA]CC
             3115  GGTGCC              
BciVI        3135  GGATAC               GTATCC(6/5)
             4112  GTATCC              
BclI          572  TGATCA               T^GATCA
             5179  TGATCA              
BfrI         2254  CTTAAG               C^TTAAG
             2982  CTTAAG              
BglI          142  GCCCTTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              191  GCCGCCGGGGC         
BlnI           43  CCTAGG               C^CTAGG
             3164  CCTAGG              
BsaAI         759  CACGTG               [CT]AC^GT[GA]
             4288  TACGTG              
BsaHI         942  GGCGCC               G[GA]^CG[CT]C
             4469  GACGCC              
BsaI         2116  GAGACC               GGTCTC(1/5)
             3101  GGTCTC              
BsgI          715  CTGCAC               GTGCAG(16/14)
              793  GTGCAG              
BsmI          300  GCATTC               GAATGC(1/-1)
             3060  GCATTC              
BsoBI        3806  CCCGAG               C^[CT]CG[GA]G
             5611  CTCGGG              
BspHI         765  TCATGA               T^CATGA
             1041  TCATGA              
BspLU11I     2962  ACATGT               A^CATGT
             5447  ACATGT              
BsrDI        2164  CATTGC               GCAATG(2/0)
             5259  GCAATG              
BstAPI        786  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1979  GCAGCAATTGC         
BstXI         782  CCACGCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4733  CCAGCATAATGG        
DsaI           96  CCGCGG               C^C[GA][CT]GG
             4145  CCATGG              
EcoT22I      3601  ATGCAT               ATGCA^T
             4256  ATGCAT              
FbaI          572  TGATCA               T^GATCA
             5179  TGATCA              
Hin1I         942  GGCGCC               G[GA]^CG[CT]C
             4469  GACGCC              
NheI         4266  GCTAGC               G^CTAGC
             4858  GCTAGC              
NsiI         3601  ATGCAT               ATGCA^T
             4256  ATGCAT              
PpuMI        1667  AGGACCT              [GA]G^G[AT]CC[CT]
             2815  GGGACCT             
PstI         1175  CTGCAG               CTGCA^G
             2152  CTGCAG              
PvuII        1996  CAGCTG               CAG^CTG
             2346  CAGCTG              
RcaI          765  TCATGA               T^CATGA
             1041  TCATGA              
StuI         4028  AGGCCT               AGG^CCT
             4236  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        ApoI        AspHI       
AvaII       BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    Bsp1407I    BspMI       
BsrFI       BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XbaI        XhoII