##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AgeI AscI AseI AsnI
Asp700I Asp718I AspI AviII BbeI
BbrPI BclI BfrI BseAI BsiWI
BspEI BspLU11I BssHII BstBI BstUI
CpoI EagI EclXI Eco52I EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KpnI KspI MluI MroI
MvnI NarI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmlI PshBI PvuI RsrII
SacII SalI ScaI SfoI SfuI
SgrAI SpeI SphI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 134 AACGTT AA^CGTT
AhdI 4326 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3958 GTGCAC G^TGCAC
Aor51HI 578 AGCGCT AGC^GCT
ApaI 2845 GGGCCC GGGCC^C
ApaLI 3958 GTGCAC G^TGCAC
AspEI 4326 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2069 CCTAGG C^CTAGG
BalI 4635 TGGCCA TGG^CCA
BamHI 2878 GGATCC G^GATCC
BcgI 3067 CGAACTGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3358 AGATCT A^GATCT
BlnI 2069 CCTAGG C^CTAGG
BlpI 3091 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3091 GCTCAGC GC^T[ACGT]AGC
BsaAI 2522 TACGTA [CT]AC^GT[GA]
BsaBI 4427 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3043 CGACCG CG[GA][CT]^CG
BsmBI 2341 GAGACG CGTCTC(1/5)
BspDI 296 ATCGAT AT^CGAT
BspHI 4659 TCATGA T^CATGA
BsrBI 2350 CCGCTC CCGCTC(-3/-3)
BssSI 1732 CTCGTG CACGAG(-5/-1)
Bst1107I 5605 GTATAC GTA^TAC
BstAPI 3802 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5605 GTATAC GTA^TAC
CelII 3091 GCTCAGC GC^T[ACGT]AGC
ClaI 296 ATCGAT AT^CGAT
Eam1105I 4326 GACTTATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 578 AGCGCT AGC^GCT
MamI 4427 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4232 CAATTG C^AATTG
MluNI 4635 TGGCCA TGG^CCA
MscI 4635 TGGCCA TGG^CCA
MunI 4232 CAATTG C^AATTG
NaeI 3648 GCCGGC GCC^GGC
NcoI 968 CCATGG C^CATGG
NgoMIV 3648 GCCGGC G^CCGGC
PmeI 5792 GTTTAAAC GTTT^AAAC
PshAI 2494 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 134 AACGTT AA^CGTT
RcaI 4659 TCATGA T^CATGA
SbfI 4717 CCTGCAGG CCTGCA^GG
SexAI 5643 ACCAGGT A^CC[AT]GGT
SfiI 4243 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2522 TACGTA TAC^GTA
Sse8387I 4717 CCTGCAGG CCTGCA^GG
SwaI 4707 ATTTAAAT ATTT^AAAT
XbaI 1294 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 980 GGCGTC G[GA]^CG[CT]C
1800 GACGCC
AocI 3686 CCTGAGG CC^T[ACGT]AGG
4067 CCTCAGG
BsaHI 980 GGCGTC G[GA]^CG[CT]C
1800 GACGCC
BsaWI 5371 TCCGGT [AT]^CCGG[AT]
5776 ACCGGA
Bsu36I 3686 CCTGAGG CC^T[ACGT]AGG
4067 CCTCAGG
DraIII 263 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
3408 CACCCAGTG
DrdI 2510 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4288 GACCACCCAGTC
DsaI 968 CCATGG C^C[GA][CT]GG
1761 CCGTGG
Eco81I 3686 CCTGAGG CC^T[ACGT]AGG
4067 CCTCAGG
EcoRI 2660 GAATTC G^AATTC
5144 GAATTC
Hin1I 980 GGCGTC G[GA]^CG[CT]C
1800 GACGCC
HindIII 598 AAGCTT A^AGCTT
4470 AAGCTT
NdeI 5286 CATATG CA^TATG
5333 CATATG
NheI 2417 GCTAGC G^CTAGC
3517 GCTAGC
SapI 3512 GAAGAGC GCTCTTC(1/4)
3838 GAAGAGC
SmaI 1044 CCCGGG CCC^GGG
1128 CCCGGG
SspI 2020 AATATT AAT^ATT
5855 AATATT
XcmI 289 CCATCGGATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2468 CCAAACTCTAGCTGG
XmaCI 1044 CCCGGG C^CCGGG
1128 CCCGGG
XmaI 1044 CCCGGG C^CCGGG
1128 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII