##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
AviII BbeI BbrPI BcgI BciVI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MroI MunI NarI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SacII SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AflII 5487 CTTAAG C^TTAAG
BamHI 5388 GGATCC G^GATCC
BbvCI 2485 CCTCAGC CCTCAGC(-5/-2)
BclI 2805 TGATCA T^GATCA
BfrI 5487 CTTAAG C^TTAAG
BsaAI 2207 TACGTG [CT]AC^GT[GA]
BsaBI 2800 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 438 GAGACG CGTCTC(1/5)
BsrBI 2672 GAGCGG CCGCTC(-3/-3)
BssHII 2058 GCGCGC G^CGCGC
EagI 31 CGGCCG C^GGCCG
EclXI 31 CGGCCG C^GGCCG
Eco52I 31 CGGCCG C^GGCCG
EcoNI 1634 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4976 GAATTC G^AATTC
EcoT22I 2379 ATGCAT ATGCA^T
FbaI 2805 TGATCA T^GATCA
MamI 2800 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2626 ACGCGT A^CGCGT
NdeI 3053 CATATG CA^TATG
NsiI 2379 ATGCAT ATGCA^T
PvuI 83 CGATCG CGAT^CG
ScaI 1324 AGTACT AGT^ACT
SexAI 2784 ACCTGGT A^CC[AT]GGT
SphI 2002 GCATGC GCATG^C
SspI 2741 AATATT AAT^ATT
XbaI 3702 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 4948 GACGTC GACGT^C
5413 GACGTC
Aor51HI 5678 AGCGCT AGC^GCT
5945 AGCGCT
AspI 854 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3090 GACAGTGTC
AvrII 2927 CCTAGG C^CTAGG
6308 CCTAGG
BlnI 2927 CCTAGG C^CTAGG
6308 CCTAGG
BlpI 1011 GCTCAGC GC^T[ACGT]AGC
5136 GCTCAGC
Bpu1102I 1011 GCTCAGC GC^T[ACGT]AGC
5136 GCTCAGC
BsgI 1122 GTGCAG GTGCAG(16/14)
2903 GTGCAG
BsmI 2951 GAATGC GAATGC(1/-1)
3220 GCATTC
BspLU11I 3457 ACATGT A^CATGT
3775 ACATGT
BsrDI 5301 CATTGC GCAATG(2/0)
2118 GCAATG
BstXI 1444 CCAACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4676 CCAAAACTCTGG
CelII 1011 GCTCAGC GC^T[ACGT]AGC
5136 GCTCAGC
DraIII 2426 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5164 CACTTTGTG
DrdI 105 GACCCCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1926 GACTGGATGGTC
Eco47III 5678 AGCGCT AGC^GCT
5945 AGCGCT
EcoRV 242 GATATC GAT^ATC
2354 GATATC
FauI 133 CCCGC CCCGC(4/6)
2361 CCCGC
HaeII 5678 AGCGCT [GA]GCGC^[CT]
5945 AGCGCT
NaeI 5021 GCCGGC GCC^GGC
5330 GCCGGC
NgoMIV 5021 GCCGGC G^CCGGC
5330 GCCGGC
NheI 4141 GCTAGC G^CTAGC
6116 GCTAGC
PflFI 854 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3090 GACAGTGTC
SalI 1780 GTCGAC G^TCGAC
5717 GTCGAC
SbfI 1130 CCTGCAGG CCTGCA^GG
2473 CCTGCAGG
SmaI 2188 CCCGGG CCC^GGG
4835 CCCGGG
Sse8387I 1130 CCTGCAGG CCTGCA^GG
2473 CCTGCAGG
StuI 805 AGGCCT AGG^CCT
2657 AGGCCT
Tth111I 854 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3090 GACAGTGTC
XmaCI 2188 CCCGGG C^CCGGG
4835 CCCGGG
XmaI 2188 CCCGGG C^CCGGG
4835 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmnI